REDOX IMAGING OF STEM CELLS

干细胞的氧化还原成像

• 批准号: 8169088
• 负责人: LIN LI
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169088
• 关键词: Apoptosis; Cardiovascular Diseases; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Disease; Flavoproteins; Funding; Grant; Image; Institution; Malignant Neoplasms; Mediator of activation protein; Metabolism; Mitochondria; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Parkinson Disease; Pathology; Reactive Oxygen Species; Regulation; Reporting; Research; Research Personnel; Resources; Scanning; Signal Transduction; Source; Stem Cell Research; Stem cells; United States National Institutes of Health; cold temperature; fluorescence imaging; in vivo; pluripotency

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellular metabolism and redox state have been shown as key mediators in cellular metabolism, apoptosis and signaling transductions. Redox state information is useful for understanding pathology of diseases including cancer, diabetes, cardiovascular disease, and Parkinson's disease etc. In addition, the regulation of reactive oxygen species and redox state has been implicated in stem cell research. However, no direct observation of mitochondrial NAD-coupled redox state in stem cells has been reported. In this project we aim to employ the low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" to image the in vivo mitochondrial redox states of stem cells and to investigate if there is any connection between mitochondrial redox state and pluripotency of stem cells.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 细胞代谢和氧化还原状态已被证明是细胞代谢的关键介质， 细胞凋亡和信号转导。氧化还原状态信息有助于理解 癌症、糖尿病、心血管疾病和帕金森病等疾病的病理学 此外，活性氧和氧化还原态的调节也已被证实。 参与干细胞研究。然而，没有直接观察到线粒体 NAD 偶联 干细胞中的氧化还原状态已有报道。 在这个项目中，我们的目标是雇用低 温度 NADH/Fp（还原型烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白） 荧光成像或“氧化还原扫描”对体内线粒体氧化还原状态进行成像 干细胞并研究线粒体氧化还原状态之间是否存在任何联系 和干细胞的多能性。