REDOX IMAGING OF STEM CELLS

干细胞的氧化还原成像

• 批准号: 8169088
• 负责人: LIN LI
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169088
• 关键词: Apoptosis; Cardiovascular Diseases; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Disease; Flavoproteins; Funding; Grant; Image; Institution; Malignant Neoplasms; Mediator of activation protein; Metabolism; Mitochondria; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Parkinson Disease; Pathology; Reactive Oxygen Species; Regulation; Reporting; Research; Research Personnel; Resources; Scanning; Signal Transduction; Source; Stem Cell Research; Stem cells; United States National Institutes of Health; cold temperature; fluorescence imaging; in vivo; pluripotency

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellular metabolism and redox state have been shown as key mediators in cellular metabolism, apoptosis and signaling transductions. Redox state information is useful for understanding pathology of diseases including cancer, diabetes, cardiovascular disease, and Parkinson's disease etc. In addition, the regulation of reactive oxygen species and redox state has been implicated in stem cell research. However, no direct observation of mitochondrial NAD-coupled redox state in stem cells has been reported. In this project we aim to employ the low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" to image the in vivo mitochondrial redox states of stem cells and to investigate if there is any connection between mitochondrial redox state and pluripotency of stem cells.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 细胞代谢和氧化还原状态已显示为细胞代谢的关键介体， 凋亡和信号传导。氧化还原状态信息对于理解很有用 包括癌症，糖尿病，心血管疾病和帕金森氏病在内的疾病病理 疾病等。此外，活性氧和氧化还原状态的调节已经 与干细胞研究有关。但是，没有直接观察线粒体nad耦合 据报道，干细胞中的氧化还原状态。 在这个项目中，我们旨在使用低 温度NADH/FP（减少烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白） 荧光成像或“氧化还原扫描”以对体内线粒体氧化还原态进行图像 干细胞并研究线粒体氧化还原状态之间是否有任何联系 和干细胞的多能性。