Flow Cytometry Laboratory (Scientific Core)

流式细胞术实验室（科学核心）

基本信息

批准号：
8336687
负责人：
Robert Wersto
金额：
$ 95.5万
依托单位：
NATIONAL INSTITUTE ON AGING
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8336687
关键词：
AM 251 Accounting Adult Age Aging Agonist Algorithms Animals Antibody Diversity Antigens Apoptosis Apoptotic Ascaridil Authorship Baltimore Bibliography Biological Assay Bone Marrow Bromodeoxyuridine CD28 gene CD8B1 gene Cardiac Myocytes Cardiovascular system Cell Cycle Cell Separation Cell surface Cells Clinical Trials Color Complex DNA Damage DNA Repair DNA biosynthesis Data Data Analyses Deaminase Development Discrimination EXO1 gene Epidermal Growth Factor Receptor Event Experimental Autoimmune Encephalomyelitis Family Flow Cytometry Frequencies Gene Expression Genetic Gerontology Head Housing Human Immune Immunofluorescence Immunologic Immunoglobulin Genes Immunology Immunosuppression Interleukin-15 Interleukin-17 Intramural Research Program Ki-67 Antigen Laboratories Ligands MC 540 Measurement Mediating Mission Mitosis Modeling Modification Molecular Molecular and Cellular Biology Mus Neurosciences Pattern Peripheral Blood Lymphocyte Pharmaceutical Preparations Physiologic pulse Planet Mars Population Population Analysis Printing Propidium Diiodide Proteins Protocols documentation Publications RNA Rattus Reporter Reporter Genes Reporting Research Research Personnel Resources Sampling Science Services Side Solutions Sorting - Cell Movement Spleen Staining method Stains Stem cells Stress Subfamily lentivirinae T cell transcription factor 1 T memory cell Training Uracil Werner Syndrome Yang base biological adaptation to stress brain cell c-myc Genes cannabinoid receptor cytokine daughter cell embryonic stem cell estrogen-related receptor follow-up helicase human embryonic stem cell immunoregulation innovation instrument mouse model nerve stem cell neurogenesis novel research study response skills small molecule thymocyte

项目摘要

While the Flow Cytometry Laboratory functions as a core for the scientific mission of the laboratories located within the NIA Intramural Research Program in Baltimore, several investigators, laboratory groups, or projects accounts for a significant portion of FY2009 resources. Usage by the Laboratory of Immunology (LI; Taub, Weng, Sen) varies between 60-70% of sorting and 50-70% of non-sorting (analytical) capacities, focusing on human and animal (mouse) models of immune status, immunoregulation, immunosuppression, and their relation to development and aging. Other supported projects involve those from the Laboratory of Cardiovascular Sciences (LCS: Boheler, ES cells and cardiomyocte differentiation; Xiao, reporter gene expression), Laboratory of Genetics (LG: Ko, patterns of ES development), Laboratory of Neuroscience (LNS: Mattson, reporter gene expression in neural stem cells and neurogenesis), Laboratory of Clinical Investigation (Bernier), Laboratory of Molecular Gerontology (LMG: Gearhart, antibody diversity and somatic hypermuations), and Laboratory of Cellular and Molecular Biology (LCMB; multiparameter cell cycle analysis). While the lab does not require co authorship on all publications for which we generate data, the following list, in addition to the bibliography below, documents publications from the IRP that include contributions from our core for this reporting period. Nguyen H, Weng NP. (2010) IL-21 preferentially enhances IL-15-mediated homeostatic proliferation of human CD28+ CD8 memory T cells throughout the adult age span. J Leukoc Biol 87:43-9. Aggarwal M, Sommers JA, Shoemaker RH, Brosh RM Jr. (2011) Inhibition of helicase activity by a small molecule impairs Werner syndrome helicase (WRN) function in the cellular response to DNA damage or replication stress.Proc Natl Acad Sci U S A. 1081525-30. Aggarwal M, Sommers JA, Morris C, Brosh RM Jr. (2010) Delineation of WRN helicase function with EXO1 in the replicational stress response. DNA Repair 9:765-76. Yu Q, Sharma A, Ghosh A, Sen JM. (2011) T cell factor-1 negatively regulates expression of IL-17 family of cytokines and protects mice from experimental autoimmune encephalomyelitis. J Immunol. 186:3946-52. Fiori J, Sanghvi M, O'Connell M, Krzysik-Walker S, Moaddel R, Bernier M. (2011). The cannabinoid receptor inverse agonist AM251 regulates expression of EGF receptor and its ligands via destabilization of estrogen-related receptor (ERR) α protein. Br J Parmacol. Mar 30. doi: 10.1111/j.1476-5381.2011.01384.x. Epub ahead of print Maul RW, Saribasak H, Martomo SA, McClure RL, Yang W, Vaisman A, Gramlich HS, Schatz DG, Woodgate R, Wilson DM 3rd, Gearhart PJ. (2011) Uracil residues dependent on the deaminase AID in immunoglobulin gene variable and switch regions.Nat Immunol. 12(1):70-6.

虽然流式细胞术实验室是位于巴尔的摩 NIA 校内研究计划内的实验室科学任务的核心，但一些研究人员、实验室小组或项目占据了 2009 财年资源的很大一部分。免疫学实验室（LI；Taub、Weng、Sen）的使用范围为 60-70% 的分选和 50-70% 的非分选（分析）能力，重点关注人类和动物（小鼠）的免疫状态模型，免疫调节、免疫抑制及其与发育和衰老的关系。其他支持的项目包括心血管科学实验室（LCS：Boheler，ES细胞和心肌细胞分化；Xiao，报告基因表达）、遗传学实验室（LG：Ko，ES发育模式）、神经科学实验室（LNS：Mattson），神经干细胞中的报告基因表达和神经发生），临床研究实验室（Bernier），分子老年学实验室（LMG：Gearhart，抗体多样性和体细胞超突变）和实验室细胞和分子生物学（LCMB；多参数细胞周期分析）。虽然实验室不要求我们为其生成数据的所有出版物都有共同作者，但除了下面的参考书目之外，以下列表还记录了 IRP 的出版物，其中包括我们核心在本报告期内的贡献。阮 H，翁 NP。 (2010) IL-21 在整个成年年龄范围内优先增强 IL-15 介导的人类 CD28+ CD8 记忆 T 细胞的稳态增殖。白细胞生物学杂志 87:43-9。 Aggarwal M, Sommers JA, Shoemaker RH, Brosh RM Jr. (2011) 小分子抑制解旋酶活性会损害细胞对 DNA 损伤或复制应激的反应中的维尔纳综合征解旋酶 (WRN) 功能。Proc Natl Acad Sci U S A. 1081525-30。 Aggarwal M、Sommers JA、Morris C、Brosh RM Jr. (2010) 在复制应激反应中描述 WRN 解旋酶与 EXO1 的功能。 DNA 修复 9：765-76。于 Q，夏尔马 A，戈什 A，森 JM。 (2011) T 细胞因子 1 负向调节细胞因子 IL-17 家族的表达，并保护小鼠免受实验性自身免疫性脑脊髓炎的侵害。 J免疫学杂志。 186：3946-52。 Fiori J、Sanghvi M、O'Connell M、Krzysik-Walker S、Moaddel R、Bernier M.（2011）。大麻素受体反向激动剂 AM251 通过破坏雌激素相关受体 (ERR) α 蛋白的稳定性来调节 EGF 受体及其配体的表达。 Br J Parmacol。 3 月 30 日。doi：10.1111/j.1476-5381.2011.01384.x。电子版先于印刷版 Maul RW、Saribasak H、Martomo SA、McClure RL、Yang W、Vaisman A、Gramlich HS、Schatz DG、Woodgate R、Wilson DM 3rd、Gearhart PJ。 (2011) 尿嘧啶残基依赖于免疫球蛋白基因可变区和开关区中的脱氨酶 AID。 12（1）：70-6。