Infrared Imaging to Assess Endothelial Function in Sickle Cell Disease Patients

红外成像评估镰状细胞病患者的内皮功能

• 批准号: 8340635
• 负责人: Alexander Gorbach
• 金额: $ 3.83万
• 依托单位: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8340635
• 关键词: Acetylcholine; Arginine; Biological Availability; Blood Vessels; Blood flow; Cells; Clinical; Computers; Coronary Angiography; Data; Data Analyses; Diagnostic; Disodium Salt Nitroprusside; Dose; Endothelium; Evaluation; Forearm; Frequencies; Functional disorder; Hand; Hemoglobin; Heterogeneity; Hyperemia; Image; Image Analysis; Imaging Techniques; Individual; Infusion procedures; Laboratories; Lighting; Measurement; Measures; Mediating; National Heart, Lung, and Blood Institute; Nitric Oxide; Patients; Pattern; Peripheral; Photography; Plethysmography; Procedures; Protocols documentation; Resistance; Sickle Cell; Sickle Cell Anemia; Skin; Spatial Distribution; Stream; Surface; System; Techniques; Temperature; Testing; Time; Vasodilation; Vasomotor; Venous; clinical application; data acquisition; in vivo; indexing; instrument; population based; preclinical study; response; software development; two-dimensional; vascular bed; vasomotion

A non-invasive, real-time, in vivo imaging procedure has been developed for directly determining the spatial distributions of blood flow, by measuring the infrared (IR) emission from the skin of an individual. The procedure does not require direct contact between the instrument and the patient. The imaging system utilizes an IR imaging camera, and a computer to store the stream of two-dimensional images and analyze data for calculating the distribution of low frequency oscillations of temperature. Patients were adapted to room temperature over 20 min and, after that, remained immobile for one min (to obtain the blood flow distribution image) and/or 5-20 min minutes during data acquisition for assessment of low frequency vasomotion. Data analyses were performed later using computer software developed in the laboratory. Measurements were made from the palmar surface of the hand or forearm. IR demonstrated dose-response patterns of spatial and temporal distribution of blood flow during acetylcholine, sodium nitroprusside and L-arginine infusions in real time. Preliminary data showed that IR- and plethysmography-derived hyperemia indexes were significantly correlated. These noninvasive tests require no special illumination, and can be used to assess microvascular recruitment and vascular tone in the periphery vascular bed.

已经开发了一种无创，实时的体内成像程序，用于直接通过测量个人皮肤的红外（IR）发射来直接确定血流的空间分布。 该过程不需要仪器与患者之间的直接接触。成像系统利用IR成像摄像头和计算机来存储二维图像的流，并分析数据以计算温度低频振荡的分布。 在20分钟内，将患者适应室温，然后在数据采集期间保持不动（以获取血流分布图像）和/或5-20分钟，以评估低频血管舒张症。 稍后使用实验室中开发的计算机软件进行数据分析。 测量由手或前臂的手掌表面进行。 IR在乙酰胆碱，亚硝普钠和L-精氨酸输注过程中表现出血流的空间和时间分布的剂量反应模式。初步数据表明，IR和散布的衍生的充血指数显着相关。这些无创测试不需要特殊的照明，可用于评估周围血管床中的微血管募集和血管张力。