Imaging of blood flow, temperature and skin color on patients with urticarias

荨麻疹患者的血流、温度和肤色成像

• 批准号: 8556172
• 负责人: Alexander Gorbach
• 金额: $ 7.99万
• 依托单位: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8556172
• 关键词: Allergic; Allergic Disease; Allergic inflammation; Area; Attenuated; Biopsy; Blood Vessels; Blood flow; Blood specimen; Cell Degranulation; Collateral Circulation; Colorimetry; Data; Dermal; Diagnosis; Documentation; Dose; Evolution; Functional Imaging; Goals; Hand; Histamine; Image; Imaging technology; Immersion Investigative Technique; Inflammatory Response; Interleukin-6; Intervention; Lasers; Measurement; Mediator of activation protein; Monitor; Optics; Patients; Pattern; Pharmaceutical Preparations; Physiologic Thermoregulation; Serum; Skin; Skin Manifestations; Temperature; Testing; Therapeutic Agents; Time; Tissues; Treatment Effectiveness; Tryptase; Urticaria; Vasodilation; cohort; hemodynamics; insight; mast cell; polarized light; response; skin color; tool

Perturbations in dermal blood flow, temperature, and skin color were analyzed using laser-speckle contrast imaging, infrared and polarized-light colorimetry following cold-hand immersion (CHI) challenges in patients with cold-induced urticaria compared to the response in healthy controls. Evidence for mast cell degranulation was established by documentation of serum histamine levels and the localized release of tryptase in post-challenge urticarial biopsies. Laser-speckle contrast imaging quantified the attenuated response to cold challenge in patients on cetirizine. We found that the vascular response provoked by mast cell degranulation is rapid and extensive. At the tissue level, it is characterized by a uniform pattern of increased blood flow, thermal warming, vasodilation, and recruitment of collateral circulation. These vascular responses are modified by the administration of an anti-histamine. Monitoring the hemodynamic responses within tissues that are a consequence of mast cell degranulation provides additional insight into the evolution of the allergic inflammatory response and offers a unique approach to assess the effectiveness of treatment intervention.

与健康对照组的反应相比，使用激光螺旋对比度成像，红外浸入（CHI）挑战后，使用激光螺旋对比度，红外和极光色的比色法分析了皮肤血流，温度和肤色的扰动。通过记录血清组胺水平和挑战后荨麻疹活检中胰蛋白酶的局部释放来确定肥大细胞脱粒的证据。 激光螺旋对比成像量化了cetirizine患者对冷挑战的减毒反应。我们发现，肥大细胞脱粒引起的血管反应是快速而广泛的。在组织水平上，它的特征是血液流动增加，热变暖，血管舒张和累积循环的募集。这些血管反应通过抗抗药性的给药来改变。 监测组织内的血液动力学反应，这是肥大细胞脱粒的结果，可以进一步了解过敏性炎症反应的演变，并提供了一种独特的方法来评估治疗干预的有效性。