PHOSPHODIESTERASE 3 INHIBITORS: BLOCKERS OF OOCYTE MATURATION AND FERTILIZATION

磷酸二酯酶 3 抑制剂：卵母细胞成熟和受精的阻断剂

基本信息

批准号：
8357748
负责人：
JEFFREY T. JENSEN
金额：
$ 3.63万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-05-01 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8357748
关键词：
Adverse effects Affect Attention Biology Chronic Contraceptive Agents Contraceptive methods Cyclic GMP Fertilization Funding Gonadotropins Grant Health Benefit Macaca mulatta Morals National Center for Research Resources Ovarian Stimulations Ovary Ovulation Partner in relationship Periodicity Pregnancy Primates Principal Investigator Protein Isoforms Protocols documentation Regulation Research Research Infrastructure Research Methodology Resources Role Source United States National Institutes of Health Unwanted Birth abortion corpus luteum cost in vivo inhibitor/antagonist novel oocyte maturation phosphoric diester hydrolase prevent selective expression unintended pregnancy

Adverse effects Affect Attention Biology Chronic Contraceptive Agents Contraceptive methods Cyclic GMP Fertilization Funding Gonadotropins Grant Health Benefit Macaca mulatta Morals National Center for Research Resources Ovarian Stimulations Ovary Ovulation Partner in relationship Periodicity Pregnancy Primates Principal Investigator Protein Isoforms Protocols documentation Regulation Research Research Infrastructure Research Methodology Resources Role Source United States National Institutes of Health Unwanted Birth abortion corpus luteum cost in vivo inhibitor/antagonist novel oocyte maturation phosphoric diester hydrolase prevent selective expression unintended pregnancy

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Contraception provides significant preventative health benefits by reducing unintended pregnancy, abortions, and unwanted births. As side effects, access, and cultural or moral objections limit the universal acceptance of current methods, research into novel contraceptives is warranted. The purpose of this project is to explore the hypotheses that selective expression of phosphodiesterase (PDE) isoforms exists in the primate ovary, that selective blockade of the PDE3 isoform can be exploited to prevent spontaneous and gonadotropin-induced oocyte maturation, and that chronic treatment with a PDE3 inhibitor can prevent pregnancy in primates without affecting menstrual cyclicity or luteal function. The Specific Aims are to: (1) describe the basic biology of PDE expression in the primate ovary, (2) determine if PDE3 inhibitors prevent oocyte maturation, but not ovulation and function of the corpus luteum in rhesus monkeys undergoing controlled ovarian stimulation (COS) protocols or during natural cycles in vivo, and 3) determine whether PDE3 inhibitors function as contraceptive agents in regularly cycling rhesus monkeys in paired mating situations. In the final year of the grant, attention was also directed to the role of cGMP-medicated regulation of PDE3.

该副本是利用资源的众多研究子项目之一由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持而且，副投影的主要研究员可能是其他来源提供的包括其他NIH来源。列出的总费用可能代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。避孕方法通过减少意外怀孕，堕胎和不必要的出生来提供重大的预防健康益处。随着副作用，访问以及文化或道德异议限制了当前方法的普遍接受，因此有必要研究对新型避孕方法的研究。该项目的目的是探索在灵长类卵巢中存在选择性表达磷酸二酯酶（PDE）同工型的假设，即可以利用PDE3同工型的选择性阻断，以防止自发性和促性腺激素诱导的卵母细胞对PDE3的影响，并防止gonadotropin诱导的pDE3对pDE3的治疗，对细胞的持续治疗，对细胞的持续治疗，对细胞的持续治疗，对细胞的持续治疗，对pDe3的持续治疗，对细胞的持续治疗，对pDE3的持续治疗，对细胞感染中的primention 3中，对细胞的持续治疗，对细胞的持续治疗，对细胞的持续治疗，对细胞感染中的himention 3循环或黄体功能。具体目的是：（1）描述灵长类动物卵巢中PDE表达的基本生物学，（2）确定PDE3抑制剂是否阻止了卵母细胞的成熟，但不能阻止芦苇在恒河猴中的岩质猴子在受控的卵巢刺激（COS）方案（cos）和3中的作用中的。在配对的情况下定期循环恒河猴。在赠款的最后一年中，人们还关注了CGMP Medicaticed PDE3的作用。