Upgrade to Confocal Facility

升级至共焦设施

• 批准号: 8247570
• 负责人: STEPHEN C MASSEY
• 金额: $ 54.34万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247570
• 关键词: Cancer Center Support Grant; Charge; Contract Services; Core Facility; Development; Diabetic Retinopathy; Family suidae; Generations; Glaucoma; Housing; Image; Journals; Knowledge; Label; Lasers; Lead; Microscope; Modeling; Noise; Ophthalmology; Peer Review; Research; Research Personnel; Retina; Retinal; Structure; Tail; United States National Institutes of Health; Vision; Vision Disorders; Visual system structure; detector; instrument; photoreceptor degeneration; programs; research study

DESCRIPTION (provided by applicant): This is an application by a group of 10 investigators to install a Zeiss LSM 780, a new generation confocal microscope suitable for multi-channel analysis of retinal circuitry and development. This model has "pig-tail" laser inputs to accommodate a variety of laser lines, high efficiency/low noise detectors and spectral analysis. These developments will make it possible to conduct 4 or 5 channel labeling experiments. The LSM 780 is self contained and menu-driven making it suitable for a multi-user facility. The instrument will be installed in the Confocal Module of the Vision Core Grant housed in the Department of Ophthalmology. This Vision Core facility is heavily used by 10 investigators conducting research on the visual system, including 8 supported by NEI, NIH programs. The service contract will be supported by the Ophthalmology department and there will be no usage charges for this group of investigators. The group is very well known for imaging studies of the retina with 6 recent cover pictures in peer-reviewed journals. In the broadest terms, we seek to understand the structure, function and development of the mammalian retina; such knowledge may lead to new strategies to alleviate visual disorders resulting from photoreceptor degeneration, glaucoma or diabetic retinopathy. PUBLIC HEALTH RELEVANCE: The mammalian retina is our window to the visual world. It is a complex self-optimizing network built from approximately 60 types of neurons. We will use multi-channel imaging techniques to discover new cell types, to relate structure to function, to decode neural circuits and to follow the steps of retinal development. A sound base of such knowledge is essential to develop new strategies to alleviate retinal conditions leading to blindness.

描述（由申请人提供）：这是由 10 名研究人员组成的小组申请安装蔡司 LSM 780，这是一种新一代共焦显微镜，适用于视网膜电路的多通道分析和开发。该型号具有“尾纤”激光输入，可容纳各种激光线、高效/低噪声探测器和光谱分析。这些进展将使进行 4 或 5 通道标记实验成为可能。 LSM 780 是独立的、菜单驱动的，使其适合多用户设施。该仪器将安装在眼科 Vision Core Grant 的共焦模块中。该 Vision Core 设施被 10 名进行视觉系统研究的研究人员大量使用，其中 8 名研究人员得到了 NEI、NIH 项目的支持。服务合同将由眼科部门支持，并且该组调查人员不会收取使用费。该小组因视网膜成像研究而闻名，最近在同行评审期刊上发表了 6 张封面图片。从最广泛的角度来说，我们试图了解哺乳动物视网膜的结构、功能和发育；这些知识可能会带来新的策略来减轻由光感受器变性、青光眼或糖尿病性视网膜病变引起的视觉障碍。 公共卫生相关性：哺乳动物视网膜是我们通向视觉世界的窗口。它是一个复杂的自优化网络，由大约 60 种神经元构建而成。我们将使用多通道成像技术来发现新的细胞类型，将结构与功能联系起来，解码神经回路并跟踪视网膜发育的步骤。良好的此类知识基础对于制定新策略来缓解导致失明的视网膜疾病至关重要。