Flow Cytometry and Imaging Shared Respource

流式细胞术和成像共享资源

• 批准号: 7698810
• 负责人: WILLIAM M GROGAN
• 金额: $ 2.81万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-03 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7698810
• 关键词: Address; Arts; Biohazardous Substance; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cell Separation; Cells; Charge; Class; Color; Complement; Computers; Contract Services; Core Facility; Cultured Cells; Custom; Data Analyses; Education; Equipment; Experimental Designs; Fingers; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence; Foundations; Fund Raising; Funding; Generations; Grant; Hour; Human Resources; Image; Image Analysis; Immunology; Individual; Institutes; Laboratories; Lasers; Methods; Microbiology; Microscope; Monograph; Numbers; Operative Surgical Procedures; Physiologic pulse; Play; Protocols documentation; Pulse taking; Quality Control; Range; Research; Research Personnel; Resource Sharing; Resources; Role; Safety; Sampling; Scanning; Services; Solid; Sorting - Cell Movement; Speed; System; Technology; Time; Tissues; Training; Training Programs; Trust; Update; Virginia; anticancer research; base; cellular imaging; design; experience; fluorescence imaging; innovation; instrument; instrumentation; novel strategies; pressure; programs; skills; user-friendly

The Flow Cytometry and Imaging Shared Resource of the Massey Cancer Center was founded in 1976. Its instrumentation has been regularly upgraded through seven generations of flow cytometers and two generations of confocal microscopes to the 4 instruments currently in use, a Coulter EPICS Elite-ESP, a Coulter XL-MCL, a Dako MoFlo high-speed analyzer/sorter and a Zeiss LSM 510 Meta imaging system. The Elite is a multi-laser flow cytometer with multiparameter sorting capabilities, operated only by Resource personnel. This instrument can be configured for a wide range of custom applications. The XL is an automated, user-friendly flow system that can be operated by authorized users of the core facility. The recently upgraded MoFlo is enclosed in a negative pressure hood providing for sorting of biohazardous materials (BSL-2+). The Zeiss imaging system is available to authorized users for confocal fluorescence imaging and analysis of cell cultures or solid tissues. The Director, William M. Grogan, has played a key role in the Resource since its founding. The Laboratory Manager, Frances White, is highly trained in flow cytometry and confocal imaging and has been with the Resource for 28 years. The Resource provides a wide range of services, from routine fluorescence analysis to interactive custom design of innovative analysis and sorting protocols, addressing the specific needs of individual investigators. Dr. Grogan closely follows the analyses performed by the core facility, insuring quality control, and advising facility users on alternative, often novel, approaches to experimental design and data analysis. Since the last review, the Resource has upgraded facilities and instrumentation, added a technician, expanded hours of operation, expanded its user training program and added the capabilities for high-speed and biohazardous sorting. This Shared Resource maintains a stable base of instrumentation, expertise and trained personnel and currently provides services and training for multiple users in 59 research groups from 21 departments at VCU. It has had a major impact on cancer research at Massey Cancer Center for 30 year.

Massey癌症中心的流式细胞仪和成像共享资源成立于1976年。 仪器定期通过7代流式细胞仪和两个 当前使用的4种仪器的几代共聚焦显微镜，Coulter Epics Elite-Esp，A Coulter XL-MCL，Dako Moflo高速分析仪/分类器和Zeiss LSM 510 Meta成像系统。这 Elite是一种具有多组分分选功能的多激光流动细胞仪，仅由资源运行 人员。可以为广泛的自定义应用程序配置该仪器。 XL是 自动化，用户友好的流系统，可以由核心设施的授权用户操作。这 最近升级的Moflo封闭在负压引擎盖中，可为生化排序 材料（BSL-2+）。 Zeiss成像系统可用于授权用户的共聚焦荧光 成像和分析细胞培养物或固体组织。导演威廉·格罗根（William M. Grogan）发挥了关键作用 自成立以来的资源中。实验室经理弗朗西斯·怀特（Frances White）接受了高度训练 细胞仪和共聚焦成像已有28年了。 该资源提供了广泛的服务，从常规荧光分析到交互式习惯 创新分析和分类协议的设计，以满足个人研究人员的特定需求。 格罗根（Grogan）博士紧密遵循核心设施进行的分析，确保质量控制并为 设施使用者采用实验设计和数据分析的替代方法（通常是新颖的方法）。自从 上次审查，该资源升级了设施和仪器，补充了一名技术人员，扩大了时间 操作，扩展其用户培训计划，并增加了高速和生物危害的功能 排序。这种共享资源维持稳定的仪器，专业知识和训练有素的人员 目前为来自21个部门的59个研究小组的多个用户提供服务和培训 VCU。它对梅西癌症中心的癌症研究产生了重大影响，已有30年了。