Imaging Module

成像模块

• 批准号: 10197135
• 负责人: STEPHEN C MASSEY
• 金额: $ 20.25万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197135
• 关键词: Animals; Antibodies; Calendar; Charge; Collaborations; Computer software; Confocal Microscopy; Contract Services; Core Facility; Core Grant; Development; Faculty; Funding; Generations; Goals; Grant; Image; Image Analysis; Instruction; Label; Learning; Letters; Libraries; Licensing; Mao-to; Microscope; Ophthalmology; Pilot Projects; Policies; Postdoctoral Fellow; Productivity; Research; Research Project Grants; Research Support; Resolution; Retinal Ganglion Cells; Running; Specialist; Students; System; Technology; Time; Training; United States National Institutes of Health; Vision; Vision research; Work; confocal imaging; cost; imager; innovation; instrument; instrumentation; member; microscopic imaging; next generation; programs; quantitative imaging; recruit; retinal imaging; transcription factor; two-photon; vision science; web page

IMAGING MODULE ABSTRACT The purpose of the Imaging Module is to provide instrumentation and training for high-resolution, multi-channel imaging and quantitative image analysis to the members of the Vision Core Group. We strive to obtain the latest technology for our confocal facility, which can support up to 5-channel labeling in a centrally located core facility accessible to all Core Grant members. In the confocal facility, we will have three Zeiss confocal microscopes, (Zeiss LSM 510 META, Zeiss LSM 780 and Zeiss LSM 800 with Airyscan (on order)) plus a Zeiss Apotome 2 mounted on an Axio Imager. After training, authorized users may sign up using the on-line calendar on the Vision Core Grant web page, which also contains instructions and a primer on multi-channel confocal microscopy. A Phoenix Research OCT system is available for small animal work. An initiative to provide 2-photon imaging has been started with a commitment of support and a goal to make this available during the first year of the program. Technical assistance and training for confocal microscopy, OCT and image analysis are available from a microscopist and software specialist supported by the Module. Programs for off-line image analysis (ZenLite, and ImageJ) are widely distributed throughout the user group and the Module holds licenses for several additional programs (ZenBlue, Imaris and Image Pro). The Module will also provide access to a comprehensive library of fluorescent secondary antibodies for multi-channel imaging. Priority use is assigned to NEI supported research and pilot projects in vision research. Because the Imaging Module will support the service contracts on the microscopes, confocal microscopy will be freely available to all members of the Vision Core Grant at no charge. We believe this is a key factor in the careful analysis of confocal material. The no-charge policy actively encourages new users and junior faculty because it allows the time to learn about confocal imaging.

成像模块 抽象的 成像模块的目的是为高分辨率、多通道提供仪器和培训 为 Vision Core Group 的成员提供成像和定量图像分析。我们努力获得 我们的共焦设施采用最新技术，可在位于中心的核心中支持多达 5 通道的标记 所有核心补助金成员均可使用该设施。在共焦设施中，我们将拥有三个蔡司共焦 显微镜（Zeiss LSM 510 META、Zeiss LSM 780 和 Zeiss LSM 800，带 Airyscan（订购））以及 安装在 Axio 成像仪上的 Zeiss Apotome 2。培训结束后，授权用户可以使用在线注册 Vision Core Grant 网页上的日历，其中还包含有关多渠道的说明和入门知识 共焦显微镜。 Phoenix Research OCT 系统可用于小动物工作。一项倡议 提供 2 光子成像已经开始，并承诺提供支持，目标是使其可用 在该计划的第一年。共聚焦显微镜、OCT 和 模块支持的显微镜专家和软件专家可以进行图像分析。节目 用于离线图像分析（ZenLite 和 ImageJ）的用户群体广泛分布，并且 模块拥有多个附加程序（ZenBlue、Imaris 和 Image Pro）的许可证。该模块还将 提供对用于多通道成像的综合荧光二抗库的访问。 优先使用 NEI 支持的视觉研究研究和试点项目。因为影像学 模块将支持显微镜的服务合同，共焦显微镜将免费提供给所有人 愿景核心补助金会员免费。我们认为这是仔细分析的一个关键因素 共焦材料。免费政策积极鼓励新用户和初级教师，因为它允许 是时候了解共焦成像了。