Acquisition of a Zeiss LSM 7 MP multiphoton microscope

购买 Zeiss LSM 7 MP 多光子显微镜

• 批准号: 8245457
• 负责人: Rodney L Parsons
• 金额: $ 59.85万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8245457
• 关键词: Blood Vessels; Brain; Calcium; Cells; Centers of Research Excellence; Cerebrum; Communication; Computer software; Core Facility; Coupling; Endothelium; Extracellular Space; Funding; Funding Applicant; Glutamates; Grant; Housing; Image; Lasers; Life; Location; Microscope; Modeling; Monitor; National Center for Research Resources; Neurons; Neurosciences; Noise; Penetration; Phase; Photons; Physiologic pulse; Physiology; Research; Research Personnel; Research Project Grants; Sapphire; Smooth Muscle; Speed; Structure; Support System; System; Time; Tissues; Training; arteriole; cell type; innovation; instrument; interest; meetings; photolysis; programs; tool; two-photon

DESCRIPTION (provided by applicant): Funds are requested to purchase a Zeiss LSM MP multiphoton microscope with a Coherent Chameleon Ultra II Ti:Sapphire pulsed laser. This instrument will replace our current BioRad Radiance Multiphoton Microscope, which no longer meets the needs of our users. In addition, Carl Zeiss is phasing out their legacy BioRad systems and no longer stocks replacement hardware or provides software upgrades for the Radiance 2300 system. The dedicated multiphoton microscope will be housed in the Imaging/Physiology Core Facility supported by the NCRR Center of Biomedical Research Excellence (COBRE) in Neuroscience grant (5P20 RR16435, 07/01/06 - 06/30/11). The competitive renewal of the COBRE grant is expected to start 7/1/01/2011 and provide 5 additional years of Core support. The Neuroscience COBRE Imaging/Physiology Core is administered by a full-time, highly trained microscopist. Acquisition of the Zeiss LSM 7 MP dedicated multiphoton microscope, a modern, modular, and actively supported system, would greatly enhance the capabilities of the Core and would meet the emerging needs of the present Core investigators and provide the opportunity for additional funded investigators to significantly expand the scope of their research programs. The new Zeiss multiphoton microscope, model LSM 7MP, offers impressively rapid acquisition speed, about 4 times higher than the speed of our BioRad Radiance 2100 MPD two-photon microscope. The high acquisition speed combined with the superior sensitivity and lower noise levels makes LSM 7 MP perfect for studying the activity of living neurons and other cell types in the brain. In recent years, multiphoton photolysis of caged compounds (uncaging) is increasingly used as a tool to quickly deliver calcium and other substances intracellularly or in the extracellular space with great precision in time, location and volume. Furthermore, multiphoton uncaging allows deeper penetration into live tissues, with smaller volumes compared to single photon photolysis. Our highly innovative research projects that investigate the neuro-vascular coupling in the brain and cerebral arteriole smooth muscle and endothelium function absolutely require the use of photolysis of caged calcium, IP3, glutamate and ATP. The Zeiss LSM 7 MP is capable of two photon photolysis localized within multiple arbitrarily defined regions of interest without slowing down the acquisition speed. This will allow us to simultaneously uncage and image living cells or tissue with a speed that is adequate for monitoring and recording the changes in cellular activity that occur as a result of uncaging. Moreover, this will enable us to simultaneously or consecutively uncage compounds in more than one cell or location thus providing us with the unique opportunity to study the interactions and communications between different cells or structures in the brain. PUBLIC HEALTH RELEVANCE: This instrument would support research designed to understand the development and normal functioning of the brain and autonomic organs and their alteration in response to disease. Imaging of cellular responses within brain slices and organs will provide key insight into the regulation of neuronal, vascular, and connective tissue function and their interdependence. This insight will inform development of therapeutic approaches for diseases that strike these tissues.

描述（由申请人提供）：要求资金购买Zeiss LSM MP Multiphoton显微镜，并具有连贯的变色龙Ultra II TI：蓝宝石脉冲激光。该仪器将取代我们当前的Biorad辐射多光子显微镜，该显微镜不再满足用户的需求。此外，卡尔·蔡司（Carl Zeiss）正在逐步淘汰其传统的biorad系统，并且不再库存更换硬件或为Radiance 2300系统提供软件升级。专用的多光子显微镜将安置在NEUROSCIENCE GRANT的NCRR生物医学研究卓越中心（COBRE）（5P20 RR16435，07/01/06-06-06-06-06/30/11）中的NCRR生物医学研究中心（COBRE）支持的成像/生理核心设施中。竞争性续签的竞争性续签预计将从2011年7月1日开始，并提供5年的核心支持。神经科学的毛cor成像/生理核心由全职，训练有素的显微镜医生管理。获取Zeiss LSM 7 MP专用的多局部显微镜（现代，模块化和积极支持的系统）将大大增强核心的能力，并将满足当前核心调查人员的新兴需求，并为其他资金的研究人员提供大量资金的调查人员的机会，从而大大扩大其研究范围 程序。新型的Zeiss多光子显微镜LSM 7MP具有令人印象深刻的快速采集速度，比我们的Biorad Radiance 2100 MPD两光子显微镜的速度高4倍。高采集速度与较高的灵敏度和较低的噪声水平相结合，使LSM 7 MP非常适合研究生物神经元和大脑中其他细胞类型的活性。近年来，笼子化合物的多光子光解（未衰老）越来越多地用作快速输送钙和其他物质细胞内或在细胞外空间中以极高的时间，位置和体积的精度传递的工具。此外，与单个光子光解相比，多光子的渗透可以使更深的渗透到活组织中，其体积较小。我们高度创新的研究项目，研究了大脑中神经血管耦合，而脑动物平滑肌和内皮功能绝对需要使用笼钙，IP3，谷氨酸和ATP的光解。 Zeiss LSM 7 MP能够在多个任意定义的利益区域内的两个光子光解，而无需降低采集速度。这将使我们能够以足够的速度同时启动和图像活细胞或组织，以监测和记录由于衰老而发生的细胞活性变化。此外，这将使我们能够在一个以上的单元或位置同时或连续地分离化合物化合物，从而为我们提供了研究不同细胞或大脑中不同细胞或结构之间的相互作用和通信的独特机会。 公共卫生相关性：该工具将支持旨在了解大脑和自主器官的发展和正常功能的研究及其对疾病的改变。大脑切片和器官中细胞反应的成像将为神经元，血管和结缔组织功能及其相互依存的调节提供关键的见解。这种见解将为造成这些组织的疾病的治疗方法开发。