Acquisition of a Yokogawa CSU-W1 Spinning Disk Confocal Microscopy System

购买横河电机 CSU-W1 转盘共焦显微镜系统

• 批准号: 8635090
• 负责人: Rodney L Parsons
• 金额: $ 51.87万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2015-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8635090
• 关键词: Bladder; Calcium; Cancer Grant Supplements (P30); Cells; Centers of Research Excellence; Confocal Microscopy; Core Facility; Coupled; Event; Funding; Funding Applicant; Housing; Image; Lasers; Life; Light; Measurement; Medicine; Microscope; Microscopy; National Center for Research Resources; National Institute of General Medical Sciences; Neurosciences; Optics; Physiology; Publications; Recording of previous events; Research; Research Personnel; Resolution; Resources; Sampling; Scanning; Signal Transduction; Speed; Support System; System; Technology; Time; Tissues; Training; charge coupled device camera; college; detector; flexibility; improved; instrument; instrumentation; lens; meetings; programs; ranpirnase

DESCRIPTION (provided by applicant): Funds are requested to purchase a Yokogawa CSU-W1 spinning disk confocal system coupled to a Nikon Eclipse NI-E upright microscope with Andor EM CCD detectors. This instrument will replace a Noran/Prairie Technology Scanning confocal microscope, which was purchased in 1995. The new instrument will be housed in the Neuroscience Imaging/Physiology Core Facility supported by the NIGMS/NCRR Center of Biomedical Research Excellence (COBRE) in Neuroscience grant (P30 RR032135/P30 GM103498, 7/1/2011-6/30/2016). The Neuroscience COBRE Imaging/Physiology Core is administered by a full-time, highly trained microscopist. Acquisition of the Yokogawa CSU W-1, a modern, modular, and actively supported system, would greatly enhance the live imaging capabilities of the Core, expanding the high-speed, high-resolution microscopy resources available to investigators within the College of Medicine and in other Colleges on the UVM campus. These investigators routinely record optical measurements of fast, localized intracellular events, such as calcium transients from living cells and tissues. The Yokogawa spinning disk will significantly improve the capabilities of the Core to meet the emerging needs of the present Core investigators, while providing the opportunity for additional funded and new investigators to expand the scope of their research programs. The Yokogawa system will use an upright rather an inverted microscope platform, allowing better access to intact thin tissues like isolated vessels or gut, bladder or urothelial strips by employing high numerical aperture physiology dipping lenses. The Yokogawa system can simultaneously record two low light signals with dual 512x512 EM CCD cameras at 52 fps (frames per second), a rate well above our other instrumentation. Higher resolution (2560X2160) imaging for brighter samples can be achieved with the Zyla sCMOS camera at 100 fps. These cameras allow great flexibility for the multiple sample types encountered in our multi-user facility. In addition, the Yokogawa system will have multiple laser lines, greatly broadening its experimental capacity by allowing simultaneous measurements of multiple fluorescent signals from defined cells. The COBRE Imaging/Physiology Core has a long history of supporting live imaging research and publication, but its instrumentation for high speed acquisition is obsolete, and can no longer meet current research demands.

描述（由申请人提供）：要求资金购买横正在旋转磁盘共聚焦系统与尼康Eclipse Ni-E直立显微镜与Andor EM CCD探测器耦合。该乐器将取代1995年购买的Noran/Prairie技术扫描共聚焦显微镜。新仪器将安置在NIGMS/NCRR生物医学研究卓越中心（COBRE）神经科学中心（COBRE）神经科学成像/生理核心设施中7/1/2011-6/30/2016）。神经科学的毛cor成像/生理核心由全职，训练有素的显微镜医生管理。获得现代化，模块化和积极支持的系统Yokogawa CSU W-1将大大增强核心的现场成像能力，扩大医学院和UVM校园其他学院的高速，高分辨率显微镜资源的扩展。这些研究者通常记录快速局部细胞内事件的光学测量，例如来自活细胞和组织的钙瞬变。横川旋转磁盘将显着提高核心能力满足当前核心调查人员的新兴需求的能力，同时为其他资助和新研究人员提供了扩大其研究计划范围的机会。横川系统将使用直立的显微镜平台，可以通过使用高数值孔径生理学浸入镜头来更好地访问完整的薄组织，例如孤立的容器或肠道，膀胱或尿路上皮条。横川系统可以同时记录两个低光信号，并以512x512 EM CCD摄像机为52 fps（每秒帧），这是我们其他仪器的速度远高于我们的速率。通过100 fps的Zyla SCMOS摄像头可以实现更高的分辨率（2560x2160）成像，以实现更明亮的样品。这些相机为我们的多用户设施中遇到的多种样本类型提供了极大的灵活性。此外，横川系统将具有多个激光线，通过允许从定义的细胞中同时测量多个荧光信号，从而大大扩展了其实验能力。毛线成像/生理学核心具有支持现场成像研究和发表的悠久历史，但是其高速获取工具已经过时，无法再满足当前的研究需求。