GENOMICS SHARED RESOURCE

基因组学共享资源

• 批准号: 8255520
• 负责人: CRAIG R TOMLINSON
• 金额: $ 10.88万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8255520
• 关键词: Bioinformatics; Biological Process; Biometry; Cancer Center Support Grant; Communities; Consultations; Core Facility; CpG Islands; Data; Experimental Designs; Freezing; Gene Expression Profiling; Gene Expression Regulation; Genetic Transcription; Genome; Genomics; Genomics Shared Resource; Goals; Heterogeneous Nuclear RNA; Knowledge; Laboratories; MicroRNAs; Nuclear; Polyribosomes; Process; RNA; RNA analysis; Research; Research Personnel; Resources; Sampling; Services; Structure; anticancer research; cost; high throughput technology; meetings; member; new technology; web site

The Genomics Shared Resource has operated since 2002 as a core facility. The GSR was developed within the NCCC as a resource to meet the research needs of the NCCC but has expanded its reach to provide services to the entire Dartmouth research community. The major objectives of the GSR are to: (1) implement state-of-the-art high-throughput technologies for the global analysis of gene regulation and expression; (2) develop a competitive low-cost structure for microarray services that would promote use by NCCC investigators; and (3) develop new technologies to exploit additional avenues in genomics. Services currently offered by the GSR include (1) analysis of RNA samples to determine quality, amount, and purity; (2) experimental design consultations in conjunction with the Biostatistics and Bioinformatics Snared Resources; (3) Affymetrix gene expression profiling; (4) Agilent gene expression profiling; (5) Agilent miRNA arrays; (6) Agilent CGH arrays from frozen and FFPE samples; and (7) Agilent CpG island arrays. Services that are to be offered in the next year include: ChlP-on-chip arrays, Agilent genome tiling arrays, and SNP analysis. Information and ordering by the investigator is available electronically via the interactive website <http://dms.dartmouth.edu/dgml/>, which was established in 2006. Some of the new technologies being developed at the GSR include the use of microarrays to determine global transcription rates, hnRNA processing, cytoplasmic turnover rates, polysome entry rates, and differential nuclear vs. cytoplasmic steady-state RNA levels. During the fiscal year ending in 2007,14 NCCC members and their laboratories (of a total of 24 laboratories at Dartmouth) used the GSR with services valued at $238,000 (-65% of $366,410 total revenues). The overall goal of the GSR is to provide NCCC researchers with state-of-the-art technical support and services for their cancer research at as low a cost as possible so that they can successfully achieve their research objectives.

基因组学共享资源自 2002 年起作为核心设施运营。 GSR 是在 NCCC 作为满足 NCCC 研究需求的资源，但已扩大其范围以提供 为整个达特茅斯研究界提供服务。 GSR的主要目标是：（1）实施 用于基因调控和表达全球分析的最先进的高通量技术； (2) 为微阵列服务开发一个有竞争力的低成本结构，以促进 NCCC 的使用 调查员； (3) 开发新技术以探索基因组学的其他途径。目前服务 GSR 提供​​的服务包括 (1) 分析 RNA 样品以确定质量、数量和纯度； (2) 与生物统计学和生物信息学 Snered 资源一起进行实验设计咨询； (3) Affymetrix基因表达谱； (4) 安捷伦基因表达谱分析； (5) 安捷伦 miRNA 芯片； (6) 来自冷冻和 FFPE 样品的安捷伦 CGH 阵列； (7) 安捷伦 CpG 岛阵列。服务是为了 明年将提供的产品包括：ChlP 芯片阵列、安捷伦基因组平铺阵列和 SNP 分析。 研究者可通过交互式网站以电子方式获取信息和订购 <http://dms.dartmouth.edu/dgml/>，成立于 2006 年。一些新技术正在 GSR 开发的技术包括使用微阵列来确定整体转录率、hnRNA 加工、细胞质周转率、多核糖体进入率以及细胞核与细胞质的差异 稳态 RNA 水平。在截至 2007 年的财政年度中，14 个 NCCC 成员及其实验室（ 达特茅斯共有 24 个实验室）使用 GSR 提供​​的服务价值为 238,000 美元（366,410 美元的-65%） 总收入）。 GSR 的总体目标是为 NCCC 研究人员提供最先进的技术 以尽可能低的成本为他们的癌症研究提供支持和服务，以便他们能够成功 实现他们的研究目标。