Genomic and Molecular Biology (GMB)

基因组和分子生物学（GMB）

基本信息

批准号：
10554247
负责人：
CRAIG R TOMLINSON
金额：
$ 20.08万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-04 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10554247
关键词：
3-Dimensional ATAC-seq Antigens Bar Codes Bioinformatics Bioinformatics Shared Resource Biological Assay Biology Biomedical Research Biometry Budgets CLIA certified Cancer Center Cancer Center Support Grant Cell Culture Techniques Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Chromium Clinical Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Communication Communities Complement Core Facility DNA DNA Sequence DNA Sequence Alteration DNA sequencing Data Data Analyses Detection Dideoxy Chain Termination DNA Sequencing Ensure Fee-for-Service Plans Funding Future Gene Expression Profiling Genome Genomics Genotype Human Resources Institution Investigation Investments Knock-in Knock-out Laboratories Leadership Marketing Measurement Messenger RNA Methodology MicroRNAs Microarray Analysis Mission Molecular Biology Molecular Biology Shared Resource Monitor Mus Norris Cotton Cancer Center Pathology Patient-Focused Outcomes Performance Pilot Projects Procedures Protein Analysis Proteins Proteomics Protocols documentation Quality Control RNA Radar Reagent Reproducibility Research Research Personnel Resource Sharing Resources Running SNP genotyping Sampling Services Speed System Technology Time Work absorption college congenic cost effective cost effective measures design digital epigenomic profiling exome experience experimental study genome-wide genomic data improved instrument instrumentation laboratory experience member nano-string new technology prevent programs protein profiling recruit research facility service providers single cell sequencing success symposium targeted sequencing technology development tool transcriptome transcriptome sequencing translational impact translational medicine whole genome

项目摘要

Project Summary/Abstract: Genomics and Molecular Biology The Genomics and Molecular Biology (GMB) Shared Resource provides advanced genomics technologies, services, and expertise in support of biomedical research conducted at the Norris Cotton Cancer Center (NCCC) and Dartmouth College. GMB operates as a fee-for-service facility offering sequencing of DNA and RNA, microarray technologies, single-cell DNA/RNA sequencing, NanoString assays, and next-day Sanger sequencing services. GMB also provides expertise for assay design and primary data analysis as complementary services. Improvements to GMB in the past several years include a new and improved sample tracking and billing system (RaDar), as well as up-grades in instrumentation and protocols used. Additional improvements include added expertise of new personnel and services for single-cell sequencing, new Nanostring assays, and mouse SNP genotyping. GMB and its committed staff have a combined 130+ years of clinical- and research-related laboratory experience. This expertise and experience facilitate GMB’s ability to operate state-of-the-art instrumentation and implement cutting-edge workflows, providing NCCC researchers with access to essential genomics technologies in this fast-moving field. It is also essential for GMB to stay competitive with market rates while providing additional value to its users over external service providers. Strong institutional support and GMB leadership ensure its stability. GMB leadership works diligently to recruit new projects to the shared resource through participation in both internal and external seminars and conferences. Personnel within GMB stay abreast of new technology developments through conference attendance as well as membership in the Association of Biomolecular Research Facilities (ABRF). GMB serves Cancer Center members, the broader Dartmouth research community, and users from outside institutions, operating at ~80% capacity. Sixty-one (61) NCCC Members from all 4 NCCC Programs utilized the GMB Shared Resource, 60 of whom are current Members in 4 Programs (CPS [6], CBT [37], ICI [14], and TEC [3]). NCCC Funded Member Use represented 31% of Total Use (4,779 Projects), and we are requesting 19% of the GMB Total Operating Budget from CCSG support. Priority is given to NCCC member samples, followed by Dartmouth samples, and then outside samples. GMB maintains stringent quality control procedures over samples entering and data leaving the facility. Appropriate controls are run regularly on all instrumentation, and run metrics are tracked over time to ensure optimal performance. Future plans involve implementing new single-cell workflows highly sought by NCCC researchers, protein and antigen barcoding, and DNA and ATAC- seq workflows. Talks are underway to acquire spatial genomic technologies, such as the Nanostring Digital Spatial Profiler, as a complement to droplet-based single-cell profiling. GMB is implementing solutions for genome-wide detection of CRISPR/Cas-9 editing, including the GUIDE-seq and CIRCLE-seq methodologies. GMB is committed to providing NCCC researchers with the latest genomics expertise and technologies.

项目摘要/摘要：基因组学和分子生物学基因组学和分子生物学 (GMB) 共享资源提供先进的基因组学技术、支持诺里斯棉花癌症中心进行的生物医学研究的服务和专业知识 (NCCC) 和达特茅斯学院作为收费服务机构运营，提供 DNA 和 DNA 测序。 RNA、微阵列技术、单细胞 DNA/RNA 测序、NanoString 检测和次日 Sanger GMB 还提供测定设计和初级数据分析方面的专业知识。过去几年对 GMB 的补充服务包括新的和改进的样本。跟踪和计费系统（雷达），以及所使用的仪器和协议的升级。改进包括增加新人员的专业知识和单细胞测序服务、新纳米串检测和小鼠 SNP 基因分型 GMB 及其忠诚的员工总共拥有 130 多年的经验。这些专业知识和经验促进了 GMB 的能力：操作最先进的仪器并实施尖端的工作流程，为 NCCC 研究人员提供在这个快速发展的领域获得必要的基因组学技术对于 GMB 的生存也至关重要。具有市场价格竞争力，同时为用户提供比外部服务提供商更多的价值。强大的机构支持和 GMB 领导层确保了 GMB 领导层的稳定性。通过参加内部和外部研讨会来共享资源的新项目 GMB 内部的人员通过会议了解新技术的发展。出席率以及生物分子研究设施协会 (ABRF) 的会员资格。癌症中心成员、更广泛的达特茅斯研究界以及外部机构的用户，来自所有 4 个 NCCC 计划的六十一 (61) 名 NCCC 会员使用了 GMB。共享资源，其中 60 名是 4 个计划的现任成员（CPS [6]、CBT [37]、ICI [14] 和 TEC [3]）。 NCCC 资助的会员使用量占总使用量（4,779 个项目）的 31%，我们要求使用量的 19% CCSG 支持的 GMB 总运营预算优先考虑 NCCC 成员样本，其次是。达特茅斯样品，然后是外部样品，GMB 保持严格的质量控制程序。对所有仪器定期运行适当的控制进入和数据离开设施。随着时间的推移跟踪运行指标，以确保未来的计划涉及实施新的最佳性能。 NCCC 研究人员高度追求的单细胞工作流程、蛋白质和抗原条形码、DNA 和 ATAC- seq 工作流程正在进行中，以获取空间基因组技术，例如 Nanostring Digital。 Spatial Profiler 作为基于液滴的单细胞分析的补充，正在实施以下解决方案。 CRISPR/Cas-9 编辑的全基因组检测，包括 GUIDE-seq 和 CIRCLE-seq 方法。 GMB 致力于为 NCCC 研究人员提供最新的基因组学专业知识和技术。