Mu Center for Botanical Interaction Studies

穆植物相互作用研究中心

基本信息

批准号：
8509482
负责人：
DENNIS B LUBAHN
金额：
$ 11.36万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-01 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8509482
关键词：
AIDS/HIV problem Acids Address Affect American Analytical Chemistry Animal Model Animal Nutrition Animals Antioxidants Arts Astrocytes Bioinformatics Biological Biological Availability Botanicals Brain Cell Culture Techniques Cells Cerebral Ischemia Chemical Structure Clinical Clinical Sciences Communicable Diseases Complex Mixtures Core Facility DNA Diet Research Disease Elderberry Environment Erinaceidae Estrogens Evaluation Event Faculty Fostering Funding Genetic Genomics Goals Grant HIV Health Healthcare Human Immune Individual Infection Inflammatory Informatics Infusion procedures Institutes Interdisciplinary Study Intervention Link Listeriosis Malignant Neoplasms Malignant neoplasm of prostate Measures Messenger RNA Methods Mission Missouri Modeling Molecular Molecular Mechanisms of Action NADPH Oxidase NF-kappa B Nerve Degeneration Neurodegenerative Disorders Neurons Outcome Oxidants Pathway interactions Phagocytes Phytochemical Phytoestrogens Phytotherapy Pilot Projects Plants Post-Translational Protein Processing Principal Investigator Property Protein Analysis Proteomics Quality Control Reactive Oxygen Species Research Research Personnel Research Project Grants Research Training Resources Role Safety Schools Scientist Sepsis Services Shapes Signal Pathway Signal Transduction Stroke Students Technology Testing The Sun Time Tissues Training Training Support Transgenic Mice Translating Translational Research Translations Universities Veterinary Medicine Work antimicrobial base bench to bedside career development clinical practice data integration dietary supplements experience high risk human disease innovation interest metabolomics new technology novel polyphenol prevent programs protective effect public health relevance secondary infection smoothened signaling pathway soy synergism

项目摘要

DESCRIPTION (provided by applicant): Botanicals are capable of interacting with several cell regulating pathways at the same time. Our Center's goal is to explore the crosstalk between antioxidant-related pathways. The 5 key pathways to be examined are the (anti)oxidant (Nrf2/Keap1 and NADPH oxidase) pathways and their interactions with the NF-kappaB-, estrogen-, and hedgehog-signaling pathways. Research Project 1: Botanicals Targeting 5 Signaling Pathways to Prevent Prostate Cancer will test the hypothesis that five individual, widely-used botanical dietary supplements will prevent prostate cancer through the five key signaling pathways. Research Proiect 2: Botanical Phenolics on Oxidative/Nitrosative Signaling Pathways: Implication for Cerebral Ischemia will test the hypothesis that botanicals promote brain health and prevent neurodegeneration through modulating the NADPH oxidase-, Nrf2/Keap1-, and/or NF-kappaB-signaling pathways, and protein modifications by S-nitrosylation. Research Project 3: Antioxidant Botanicals and Antimicrobial Defenses will test the hypothesis that botanical compounds that possess potent antioxidant activity modulate cellular events associated with antimicrobial defense. The Center includes five core facilities. The Administrative Core will oversee the Center's research, training, and pilot programs. Core A: Botanicals/Plant Genomics Core will identify, cultivate, and document raw plant materials including novel cultivars of elderberry and soy. Core B: Nutrition/Animal Core will provide transgenic mice and prepare diets for the research projects. Core C: Analytical Chemistry Core will provide analytical support and quality control for quantifying botanical compounds and their metabolites, and verifying plant material. Core D: Interactions Core: Mega-sequencing/Proteomics/lnformatics/Nitrosylation) will provide novel technology for proteomic and nitrosylation analyses, DNA mega-sequencing for mRNA analysis, and data integration through bioinformatics and statistical support. MU is highly supportive of this Center and is providing funds to train and support four graduate research assistants. Pilot projects will help provide seed money for new botanical grants and allow career development of both new faculty and students. Our Center's approach using bioinformatics to explore 5 signaling pathways concurrently by the latest cutting edge technology in mRNA and protein analysis is highly innovative. This type of synergism, when combined with the botanical and pathway expertise of Center investigators, will generate new information important in human health. PUBLIC HEALTH RELEVANCE: The botanicals we propose to study are already extensively used by the public, and though they have antioxidant properties that may offer cancer, immuno-, and neuroprotective effects, the molecular basis of these effects is largely not understood. Our overall goal is to test and identify botanicals and related molecular mechanisms that can potentially offer beneficial effects to maintain a healthy body and brain by preventing neurodegenerative and infectious diseases, as well as cancer.

描述（由申请人提供）：植物能够同时与几个细胞调节途径相互作用。我们中心的目标是探索与抗氧化剂相关的途径之间的串扰。要检查的5个关键途径是（抗）氧化剂（NRF2/KEAP1和NADPH氧化酶）途径及其与NF-kappab-，雌激素和刺猬信号途径的相互作用。研究项目1：针对5种预防前列腺癌的5个信号传导途径的植物会将检验以下假设：五个单独使用的植物饮食补充剂将通过五个关键的信号通路来防止前列腺癌。研究研究2：对氧化/亚硝化信号通路的植物酚类：对脑缺血的影响将检验以下假说：植物学促进脑健康并通过调节NADPH氧化酶 - 通过NRF2/KEAP1-，以及/或NF-kapbababababababbababsinition和STORTICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICICATICATIC。研究项目3：抗氧化剂植物药和抗菌防御能力将检验以下假设：具有有效抗氧化活性的植物化合物调节与抗菌防御相关的细胞事件。该中心包括五个核心设施。行政核心将监督该中心的研究，培训和试点计划。核心A：植物/植物基因组学核心将识别，培养和记录原始植物材料，包括新颖的接骨木浆果和大豆品种。核心B：营养/动物核心将为研究项目提供转基因小鼠并为饮食准备饮食。核心C：分析化学核心将提供分析支持和质量控制，以量化植物化合物及其代谢产物，并验证植物材料。核心D：相互作用核心：巨型测序/蛋白质组学/lnformatics/硝基化）将为蛋白质组学和硝基化分析，DNA MEGA序列提供新颖的技术，用于mRNA分析以及通过生物信息信息和统计支持的数据整合。 MU非常支持该中心，并提供资金来培训和支持四名研究生研究助理。试点项目将有助于为新的植物学赠款提供种子资金，并允许新教师和学生的职业发展。我们中心使用生物信息学在mRNA和蛋白质分析中同时探索5个信号通路的方法是高度创新的。这种类型的协同作用与中心研究人员的植物学和途径专业知识相结合，将产生对人类健康重要的新信息。公共卫生相关性：我们建议研究的植物药已经被公众广泛使用，尽管它们具有可能提供癌症，免疫和神经保护作用的抗氧化特性，但这些作用的分子基础在很大程度上尚不清楚。我们的总体目标是测试和鉴定植物和相关的分子机制，这些机制可能通过预防神经退行性和传染病以及癌症来潜在地提供有益的身体和大脑。