The Interactions Core/Mark Mcintosh

交互核心/马克·麦金托什

• 批准号: 8007179
• 负责人: DENNIS B LUBAHN
• 金额: $ 22.51万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007179
• 关键词: Affect; Anti-Inflammatory Agents; Anti-inflammatory; Antioxidants; Biochemical; Bioinformatics; Biological; Biometry; Botanicals; Complement; Cysteine; DNA; Data; Data Set; Disease; Disease model; Erinaceidae; Estrogens; Funding; Gel; Gene Expression Regulation; Gene Proteins; Genome; Genomics; Goals; Human Resources; Informatics; Label; Libraries; Maps; Mass Spectrum Analysis; Measures; Messenger RNA; Methods; Modeling; Modification; Molecular; NADPH Oxidase; Output; Oxidation-Reduction; Pathway interactions; Plasma; Post-Translational Modification Site; Post-Translational Protein Processing; Process; Production; Proteins; Proteomics; Protocols documentation; Regulation; Relative (related person); Research Personnel; Research Project Grants; Route; Sampling; Scientist; Screening procedure; Signal Pathway; Signal Transduction; Site; Stress-Induced Protein; Sulfhydryl Compounds; Systems Biology; Technology; Transduction Gene; Validation; Work; base; instrument; network models; nitrosative stress; protein expression; response

The Interactions Core: Mega-sequencing, Proteomics, Informatics and Nitrosylation will provide proteomic analysis, mRNA mega-sequencing, bioinformatic analyses, and biostatistics to support the research projects. For proteomic analysis of antioxidant, anti-inflammatory, NADPH oxidase, hedgehog, and estrogen signaling pathway targets, affecting protein expression levels and redox-based protein post-translational modifications (PTM), particularly S-nitrosylation on specific protein cysteine thiols, we will collaborate with MU's Charles W. Gehrke Proteomics Center to develop an integrative proteomics strategy including both gel- and LC/MSbased quantitative proteomics. We will develop protein cysteine post-translational modification PTM site mapping using CID/ETD technology and provide our expertise to identify potential redox targets and aid in mechanistic studies. High-throughput mRNA mega-sequencing will also be completed by personnel in this core. We will work with the MU DNA Facility and its ultra-high throughput sequencing platform. Sequence data output will be routed directly to the bioinformatician for initial processing, library construction, and screening. In later years of the funding period, we will develop protocols to look at protein-level changes in plasma. Both genomic and proteomic data will be integrated into a pathway interaction model that will provide hypotheses for experimental validation. Overall the Core will provide support to the research projects and work to develop a deeper understanding of how botanicals can influence signaling pathways.

互动核心：大型测序，蛋白质组学，信息学和亚硝基化将提供蛋白质组学 分析，mRNA大型测序，生物信息学分析和生物统计学以支持研究项目。 用于抗氧化剂，抗炎，NADPH氧化酶，刺猬和雌激素信号的蛋白质组学分析 途径靶标，影响蛋白质表达水平和基于氧化还原的蛋白质后翻译后修饰 （PTM），特别是对特定蛋白质半胱氨酸硫醇的S-亚硝基化，我们将与MU的Charles合作 W. gehrke蛋白质组学中心开发了一个综合蛋白质组学策略，包括凝胶和LC/MSBSB 定量蛋白质组学。我们将开发蛋白质半胱氨酸后翻译后PTM位点 使用CID/ETD技术进行映射，并提供我们的专业知识，以确定潜在的氧化还原目标并帮助 机械研究。高通量mRNA mega-sequing也将由人员完成 核。我们将使用MU DNA设施及其超高吞吐量测序平台。顺序 数据输出将直接路由到生物信息学家进行初始处理，库构建和 筛选。在资金期的后期，我们将制定协议以查看蛋白质级别的变化 等离子体。基因组和蛋白质组学数据都将集成到途径相互作用模型中，该模型将 提供实验验证的假设。总体而言，核心将为研究项目提供支持 并努力对植物会如何影响信号通路有更深入的了解。