Analytical Chemistry Core/George Rottinghaus

分析化学核心/George Rottinghaus

• 批准号: 8007178
• 负责人: DENNIS B LUBAHN
• 金额: $ 10.04万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8007178
• 关键词: Analytical Chemistry; Animal Nutrition; Apocynum; Blood Circulation; Body Fluids; Botanicals; Cysteine; Diet; Elderberry; Ensure; Equipment; Excretory function; Genomics; Human Resources; Individual; Laboratory Personnel; Materials Testing; Measurement; Measures; Metabolism; Methodology; Methods; Phytoestrogens; Picrorhiza; Plants; Plasma; Procedures; Protocols documentation; Quality Control; Research; Research Design; Research Project Grants; Research Subjects; Sampling; Screening procedure; Services; Specific qualifier value; Techniques; Testing; Training; Training Support; Work; acetovanillone; cost; graduate student; potency testing; research study; soy

The Analytical Chemistry Core will provide analytical support for the Research Projects and the Botanical/Plant Genomics and Nutrition/Animal Cores. The facility will minimize equipment and personnel costs, provide expert technical support, training, and quality control. The specific aims ofthe facility are to: (1) develop and/or validate analytical procedures for quantifying active medicinal compounds and their metabolites; (2) provide analytical quantitation for quality control of diets prepared by the Nutrition/Animal Core for the Research Projects and screening of botanical samples for the Botanical/Plant Genomics Core; and (3) measure circulating levels of active medicinal compounds and their metabolites in body fluids of research subjects for the Research Projects. The Analytical Chemistry Core will evaluate and validate analytical methodology for testing phytoestrogens in soy, S-sustituted cysteine derivatives in gariic, sutheriandiosides and sutheriandins in Lessertia frutescens (also known as Sutheriandia frutescens), apocynin in Apocynum and Picrorhiza species, and the phenolics in elderberry. All botanical test materials, commercial compounds, and diets will be routinely examined for purity and/or active or marker compounds. Elderberry, soy and apocynin-producing botanicals will be screened for active constituents to identify high and low-potency materials for research studies. Circulating levels of botanical active compounds or their metabolites will provide valuable information for the three Research Projects.

分析化学核心将为研究项目以及植物/植物基因组学和营养/动物核心提供分析支持。该设施将最大程度地减少设备和人员成本，提供专家技术支持，培训和质量控制。该设施的具体目的是： （1）开发和/或验证分析程序，以量化活性药物化合物及其代谢产物； （2）为营养/动物核心制备的饮食质量控制提供了分析定量，用于研究项目，并筛选植物/植物基因组学核心的植物样品； （3）测量研究项目研究对象的体液中活性药物化合物及其代谢产物的循环水平。分析化学核心将评估和验证分析方法，以在大豆，S源性的半胱氨酸衍生物中的植物雌激素中的GARIIC，Sutheriandiosides和Sutheriandins中的sutheriandia frutescens（也称为Sutheriandia frutescens）中的Sutheriandiosides和Sutheriandins进行测试。所有植物测试材料，商业化合物和饮食都将经常检查纯度和/或活性或标记化合物。 接骨木浆果，大豆和偶氮蛋白产生的植物会将被筛选为活跃成分的成分，以鉴定研究研究材料的高功效材料。植物活性化合物或其代谢产物的循环水平将为三个研究项目提供有价值的信息。