Analytical Cytochemistry

分析细胞化学

• 批准号: 8518642
• 负责人: THEODORE G KRONTIRIS
• 金额: $ 14万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2013-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8518642
• 关键词: Archives; Back; Biological Assay; Budgets; Calibration; Cancer Center; Cancer Center Support Grant; Cells; Computer software; Cytometry; Data; Development; Equipment; Fees; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Goals; Image; Label; Laboratories; Laser Scanning Cytometry; Lasers; Libraries; Logistics; Maintenance; Management Information Systems; Measures; Microscopic; Network-based; Peer Review; Population; Preparation; Preventive; Process; Property; Publications; Quality Control; Research Personnel; Resources; Retrieval; Running; Scanning; Screening procedure; Services; Sorting - Cell Movement; Speed; Technology; Time; Tissues; Training; base; computerized data processing; cost; cytochemistry; experience; instrumentation; light scattering; member; operation; programs; research study; tool

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.

分析细胞仪核心（ACC）的目的是提供领先的设备和经验丰富的操作员来测量和分离物，细胞及其组件的性质，并提供数据以进行内部分析和外部审查。该核心资源中的流式细胞仪仪器包括（1）高速细胞分散器（MOFLO）和（2）分析细胞仪（CYAN和FACSCALIBUR）。流式细胞仪仪器根据多个荧光标签和高产量的光散射特性（根据速度，最高90％），以高达35,000个电池/秒的速度为研究人员提供了分析和分离细胞的工具，以高达35,000个电池/秒的速度。 和极端纯度（99％）。由于可以在多个参数上对四个人群进行分类的能力，因此研究人员的物流和成本大大降低。 CYAN分析仪的新的高吞吐量板采样器为具有大型化合物库的研究者提供了快速筛选能力，这些库在化合物鉴定中使用流式细胞仪分析；激光扫描细胞仪（LSC）的添加已扩大了ACC提供的分析能力，包括基于定量组织 荧光显微镜。该仪器同时收集了ListMode荧光数据和微观图像，并为用户提供了将定量荧光数据与大型组织部分上的定性显微镜图像相关联的能力。 LSC由经过特殊培训的用户运行，并由专门的操作员管理。 ACC仪器将受到每周质量控制评估以及常规的预防性维护和校准的约束。核心中生成的数据可通过BRI-NET服务器获得，以进一步分析和准备演示或出版物。基于网络的数据处理软件由核心提供，并且正在开发实验室信息管理系统（LIMS），以帮助跟踪与实验相关的元数据和存档文件检索。在2006财年期间，来自所有5个计划和3个不结盟成员的44名癌症中心成员使用了ACC（64％的同行评审使用）。 该核心的年度预算为$ 401,799（机构43％，用户费用40％）； CCSG要求17％（$ 70,100）。