Analytical Cytochemistry

分析细胞化学

• 批准号: 8396534
• 负责人: THEODORE G KRONTIRIS
• 金额: $ 9.19万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2013-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8396534
• 关键词: Archives; Back; Biological Assay; Budgets; Calibration; Cancer Center; Cancer Center Support Grant; Cells; Computer software; Cytometry; Data; Development; Equipment; Fees; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Goals; Image; Label; Laboratories; Laser Scanning Cytometry; Lasers; Libraries; Logistics; Maintenance; Management Information Systems; Measures; Microscopic; Network-based; Peer Review; Population; Preparation; Preventive; Process; Property; Publications; Quality Control; Research Personnel; Resources; Retrieval; Running; Scanning; Screening procedure; Services; Sorting - Cell Movement; Speed; Technology; Time; Tissues; Training; base; computerized data processing; cost; cytochemistry; experience; instrumentation; light scattering; member; operation; programs; research study; tool

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.

分析细胞计数核心 (ACC) 的目标是提供先进的设备和经验丰富的操作员来测量和分离细胞及其成分的特性，并提供数据以供内部分析和外部审查。该核心资源中的流式细胞仪包括 (1) 高速细胞分选仪 (MoFlo) 和 (2) 分析细胞仪（Cyan 和 FacsCalibur）。流式细胞术仪器为研究人员提供了基于多种荧光标记和高产率光散射特性，以高达 35,000 个细胞/秒的速度分析和分离细胞的工具（基于速度高达 90%） 和极高的纯度（99%）。由于能够根据多个参数一次对四个群体进行分类，因此研究人员的后勤和成本大大降低。用于青色分析仪的新型高通量板采样器为拥有大型化合物库、在化合物鉴定中使用流式细胞术分析的研究人员提供了快速筛选能力；添加激光扫描细胞仪 (LSC) 扩展了 ACC 提供的分析能力，包括基于组织的定量分析 荧光显微镜。该仪器收集列表模式荧光数据和显微图像，并为用户提供将定量荧光数据与大组织切片上的定性显微图像相关联的能力。 LSC 由经过专门培训的用户运行并由专门的操作员管理。 ACC 仪器每周接受质量控制评估以及日常预防性维护和校准。核心中生成的数据可通过 BRI-net 服务器进行进一步分析和准备演示或发布。核心提供基于网络的数据处理软件，并且正在开发实验室信息管理系统（LIMS），以帮助跟踪与实验相关的元数据和存档文件检索。 2006 财年期间，来自所有 5 个项目的 44 名癌症中心成员和 3 名不结盟成员使用了 ACC（64% 的同行评审使用率）。 该核心的年度预算为 401,799 美元（43% 为机构费用，40% 为用户费用）；向 CCSG 请求 17%（70,100 美元）。