Determination of biomarkers of iron status and inflammation in saliva.

唾液中铁状态和炎症生物标志物的测定。

• 批准号: 10303822
• 负责人: Saurabh Mehta
• 金额: $ 23.55万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-09 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10303822
• 关键词: Accounting; Acids; Active Biological Transport; Address; Affect; Age; Anemia; Archives; Back; Base Ratios; Biological; Biological Assay; Biological Markers; Blood; Blood specimen; Body measure procedure; Breast Feeding; Bypass; C-reactive protein; Centers for Disease Control and Prevention (U.S.); Child; Collection; Communities; Development; Diagnostic; Diffusion; Elderly; Equation; Equipment; Ferritin; Food; Future; Goals; HSV glycoprotein C; Health; Heterogeneity; Human Resources; India; Individual; Inflammation; Inflammatory; Infrastructure; Intake; International; Intervention; Iron; Lactation; Link; Malnutrition; Measures; Methods; Morbidity - disease rate; Mothers; National Health and Nutrition Examination Survey; Nutrient; Nutrition Assessment; Nutritional; Nutritional status; Orosomucoid; Outcome; Oxygen; Pennisetum; Performance; Physiological; Population; Production; Program Evaluation; Proteins; Public Health; Randomized; Research; Resistance; Resources; Risk; Saliva; Salivary; Sampling; Serum; Site; Surveillance Program; Surveys; TFRC gene; Testing; Training; Ultrafiltration; Wheat; Woman; Work; base; biomarker selection; blood-based biomarker; cohort; community setting; cooking; cost efficient; diagnostic accuracy; feeding; hepcidin; high risk; immune function; improved; indexing; innovation; iron deficiency; mortality; nutrition; point-of-care diagnostics; population survey; pregnant; reproductive; research and development; response; saliva sample; salivary assay; sample collection

PROJECT SUMMARY Biomarker determination for both nutritional status and inflammation has classically relied on blood-based biomarkers. Blood sampling has associated challenges including being partially invasive, requiring specially trained personnel and equipment for collection and processing, and having resistance to blood collection in some communities. Our long-term goal is to evaluate salivary biomarkers as an option to address issues common with blood sampling. Our overall objectives in this application are to determine salivary and serum biomarkers of iron and inflammation status in a population with expected burden of malnutrition and inflammation. We hypothesize that salivary biomarkers will demonstrate sufficient diagnostic performance for determining the degree of inflammation and iron status relative to serum biomarkers. We will test our hypothesis with the following specific aims: 1) In a resource-limited setting with heterogeneity in iron status, compare salivary versus serum biomarkers accounting for inflammation, 2) Evaluate the utility of saliva for determination of the ratio-based body iron index, and 3) Measure changes in salivary biomarkers and the body iron index in response to an iron intervention. Our approach includes domestic pilot work and leveraging a cohort of mothers and their young children in India participating in a feeding trial including biofortified high iron pearl millet. We also have paired serum-saliva samples from a survey of women of reproductive age participating in a CDC-supported periconceptional surveillance program at the same site in India as a potential back up. In our opinion, this research is innovative because the development of salivary biomarkers of inflammatory and nutritional status, including the ratio-based body iron index, would facilitate easier, cheaper, and more acceptable sampling and assessment of an individual’s or population’s status. These contributions are significant because they could dramatically improve biological sampling and assessment for nutritional and inflammatory assessment, which remain significant public health burdens globally and have challenges with assessment of high-risk individuals. Such developments would open up future avenues of research and development in salivary biomarkers and diagnostics.

项目概要 营养状况和炎症的生物标志物测定传统上依赖于血液 生物标记物采样具有相关的挑战，包括部分侵入性、需要特殊的要求。 经过培训的人员和设备进行采集和处理，并且在某些情况下对血液采集有抵抗力 我们的长期目标是评估唾液生物标志物作为解决常见问题的一种选择。 我们在此应用中的总体目标是测定唾液和血清中铁的生物标志物。 以及预期有营养不良和炎症负担的人群的炎症状况。 唾液生物标志物将表现出足够的诊断性能来确定唾液的程度 与血清生物标志物相关的炎症和铁状态我们将用以下具体方法来检验我们的假设。 目标：1) 在铁状态存在异质性的资源有限环境中，比较唾液与血清生物标志物 考虑炎症，2) 评估唾液对于确定基于比率的身体铁指数的效用， 3) 测量唾液生物标志物和身体铁指数对铁干预的反应。 我们的方法包括国内试点工作以及利用印度的一群母亲及其年幼的孩子 参加一项包含生物强化高铁珍珠小米的喂养试验，我们还有配对的血清-唾液。 来自参与 CDC 支持的围孕期育龄妇女调查的样本 我们认为，这项研究是创新的，在印度同一地点进行的监视计划作为潜在的备份。 因为炎症和营养状况的唾液生物标志物的发展，包括基于比率的 身体铁指数，将有助于更容易、更便宜和更容易接受的采样和评估 这些贡献非常重要，因为它们可以显着改善个人或群体的状况。 用于营养和炎症评估的生物采样和评估，这仍然是重要的公众 此类发展将给全球带来健康负担，并对高风险个人的评估带来挑战。 开辟唾液生物标志物和诊断学的未来研究和开发途径。