ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS AND PIGTAIL MACAQUE COLONIES

无特定病原体的恒河猴和尾猴群体的建立

• 批准号: 7958424
• 负责人: Michael K Axthelm
• 金额: $ 20.67万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-04 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958424
• 关键词: AIDS Vaccines; AIDS-Related Opportunistic Infections; Acquired Immunodeficiency Syndrome; Adolescent; Adult; Alleles; Animal Experimentation; Animal Model; Animals; Area; Breeding; Cercopithecine Herpesvirus 1; Chinese People; Computer Retrieval of Information on Scientific Projects Database; Cytomegalovirus; Disease; Female; Funding; Future; Genetic; Genetic Variation; Grant; Housing; Human Virus; Immunocompromised Host; Infection; Institution; Lentivirus Infections; Lymphocryptovirus; Macaca; Macaca mulatta; Macaca nemestrina; Maintenance; Major Histocompatibility Complex; Pathogenesis; Population; Primates; Recruitment Activity; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Rhadinovirus; SIV; Shelter facility; Simian Retroviruses; Simian T-lymphotropic virus 1; Simian virus 40; Source; Specific Pathogen Frees; Target Populations; United States National Institutes of Health; germ free condition; meetings; nonhuman primate; offspring; programs; square foot; supported housing

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Opportunistic human viruses and their simian counterparts are similar in their genetic makeup and induce a similar spectrum of diseases in their immunosuppressed hosts. Experimental lentivirus infection in rhesus macaques is widely recognized as the most important animal model for AIDS-related research and there is an urgent need to expand breeding programs to meet future AIDS vaccine and pathogenesis research program demands. The objective of this project is to enhance infrastructure for AIDS-related research in nonhuman primates by developing an expanded definition SPF Indian rhesus macaque breeding colony to support research focused on AIDS-related opportunistic infections. The Center's current population of expanded SPF rhesus macaques will be increased to a target population of 100 adult breeder females by annually recruiting 10 extensively screened juvenile females from the Center's SPF Indian rhesus macaque resource and retaining female offspring. Specific aims include: 1) establishing and managing an expanded SPF Indian-origin rhesus macaque breeding colony free of cytomegalovirus, rhadinovirus, lymphocryptovirus, Cercopithecine herpesvirus 1, simian virus 40, simian T lymphotropic virus 1, D type simian retroviruses, simian immunodeficiency virus and spumaretrovirus (foamy) infections; 2) maintenance of colony genetic diversity and major histocompatibility complex (MHC) typing for selected class I and II alleles; 3) purchase of 100 Chinese rhesus macaques to develop a Chinese rhesus macaque breeding colony that will supply research animals to Center research programs that do not require animals with an Indian genetic background; 4) renovation of 4,030 sq. ft. of shell space to provide indoor group housing and support areas separate from other macaques to protect the expanded SPF colony; and 5) purchase and installation of additional outdoor Shelter Group Housing modules to provide adequate housing for rhesus macaques produced in the U42-supported SPF AIDS Research Breeding Colony.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 机会性人类病毒和它们的猿猴病毒在基因组成上相似，并在免疫抑制的宿主中诱发相似的疾病谱。恒河猴实验性慢病毒感染被广泛认为是艾滋病相关研究最重要的动物模型，迫切需要扩大育种计划以满足未来艾滋病疫苗和发病机制研究计划的需求。该项目的目标是通过开发扩展定义的 SPF 印度恒河猴繁殖群来加强非人类灵长类动物艾滋病相关研究的基础设施，以支持重点关注艾滋病相关机会性感染的研究。通过每年从该中心的 SPF 印度恒河猴资源中招募 10 只经过广泛筛选的幼年雌性猕猴并保留雌性后代，该中心目前的扩大 SPF 恒河猴种群数量将增加到 100 只成年雌性饲养猕猴的目标种群。具体目标包括：1) 建立和管理一个扩大的 SPF 印度原产恒河猴繁殖群，不含巨细胞病毒、鼻病毒、淋巴隐病毒、猴疱疹病毒 1、猿猴病毒 40、猿猴 T 淋巴细胞病毒 1、D 型猿猴逆转录病毒、猿猴免疫缺陷病毒和泡沫逆转录病毒（泡沫状）感染； 2) 维持选定的 I 类和 II 类等位基因的集落遗传多样性和主要组织相容性复合体 (MHC) 分型； 3) 购买100只中华恒河猴，建立一个中华恒河猴繁殖群，为中心研究项目提供研究动物，这些项目不需要具有印度遗传背景的动物； 4) 翻新 4,030 平方英尺的外壳空间，提供与其他猕猴分开的室内群体住房和支持区域，以保护扩大的 SPF 群体； 5) 购买和安装额外的户外庇护所组舍模块，为 U42 支持的 SPF 艾滋病研究繁殖群中生产的恒河猴提供足够的住房。