Animal Model and Sample Analysis Core

动物模型和样品分析核心

基本信息

批准号：
8403442
负责人：
JULIA K L WALKER
金额：
$ 36.8万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2013-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8403442
关键词：
Air Allergens Animal Experimentation Animal Model Animals Asthma Biological Biological Assay Bleomycin Bronchoalveolar Lavage Bronchoalveolar Lavage Fluid Cell Culture Techniques Cells Cellular biology Chronic Collection Data Quality Databases Development Doctor of Medicine Doctor of Philosophy Epithelial Cells Equipment Evaluation FDA approved Fibroblasts Fibrosis Goals Harvest Human Resources Hyaluronan Inflammatory Instruction Integration Host Factors Lead Liquid substance Lung Lung Inflammation Lung diseases Measurement Mechanics Medical Messenger RNA Methods Modeling Molecular Biology Mus Outcome Performance Phenotype Play Preparation Procedures Program Research Project Grants Proteins Pulmonary Fibrosis Regulation Research Personnel Resources Respiratory physiology Role Sampling Specimen Structure of parenchyma of lung Time Tissue Procurements Tissues Walkers Work airway remodeling asthmatic airway cost experience improved in vivo interstitial macrophage matrigel mouse model novel therapeutics programs repository

项目摘要

The purpose of Core B - Mouse Models of Fibroproliferative Lung Disease - is to provide a resource for investigators to conduct studies in mice. The Director of Core B, Julia K. L. Walker, Ph.D., is a physiologist who has extensive experience with mouse models of lung inflammation. The Co-director, Dianhua Jiang, M.D., Ph.D., is an expert in lung cell and molecular biology who has extensively studied mechanisms that control lung flbrosis. A major strength of this program project grant is that each project is centered around the common theme that endogenous matrix components drive a pro-flbrofic fibroblast phenotype and that this underiies fibroproliferative lung diseases. By conducting standardized mouse models of pulmonary fibroproliferation, including a bleomycin model of pulmonary fibrosis and a chronic allergen-induced model of asthmatic airway remodeling, the Animal Core will facilitate interactions among the three projects and enhance their synergy. Additionally, the Core will coordinate histological preparation and analysis of fixed lung so that this evaluation will also be standardized. The Animal Core will work with investigators to provide lung fissue from which primary fibroblast and other cell cultures can be made. In summary, the Animal Core will provide the investigators with a facility where mice, experimental equipment and methods of analysis are consolidated and that is supported by skilled staff with expertise in all aspects of lung disease development and assessment. Centralizing the animal work in the Animal Core will reduce the cost of producing high-quality data and will facilitate interactions among the different projects. Therefore, the Animal Core will play an important role in the successful completion of work proposed in this Program Project.

Core B（纤维增生性肺病小鼠模型）的目的是为研究人员在小鼠中进行研究提供资源。 Core B 的主任 Julia K. L. Walker 博士是一位生理学家，在小鼠肺部炎症模型方面拥有丰富的经验。联合主任蒋殿华医学博士、哲学博士是肺细胞和分子生物学专家，对肺纤维化的控制机制进行了广泛的研究。该计划项目资助的一个主要优势是每个项目都围绕共同的主题是内源性基质成分驱动促纤维化成纤维细胞表型，并且这是纤维增殖性肺疾病的基础。通过建立肺纤维增殖的标准化小鼠模型，包括博莱霉素肺纤维化模型和慢性过敏原诱导的哮喘气道重塑模型，动物核心将促进三个项目之间的相互作用并增强它们的协同作用。此外，核心将协调固定的组织学准备和分析肺，使这一评估也将标准化。动物核心将与研究人员合作提供肺组织，从中可以制备原代成纤维细胞和其他细胞培养物。总之，动物核心将为研究人员提供一个整合小鼠、实验设备和分析方法的设施，并得到在肺部疾病发展的各个方面具有专业知识的熟练工作人员的支持和评估。将动物工作集中在动物核心中将降低生成高质量数据的成本，并将促进不同项目之间的互动。因此，动物核心将在本计划项目的顺利完成中发挥重要作用。