DEVELOPING NANOPORES AS NANOSENSORS

开发纳米孔作为纳米传感器

• 批准号: 8172031
• 负责人: Aleksei Aksimentiev
• 金额: $ 5.02万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172031
• 关键词: Charge; Collaborations; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Sequence; Devices; Funding; Grant; Hemolysin; Institution; Ions; Membrane; Modeling; Nature; Nucleic Acids; Physics; Polymers; Radial; Research; Research Personnel; Resources; Silicon; Solutions; Source; Stretching; Techniques; United States National Institutes of Health; Work; aqueous; base; improved; nanofluidic; nanopore; nanoscale; nanosensors; protein complex; sensor; usability; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nanopores are small pores with nanometer-scale radii. They are found in nature, such as the proteinaceous alpha-hemolysin pore [1], but can also be artificially manufactured in silicon-based membranes [2] and polymer wafers [3]. When immersed in aqueous solution and with an applied voltage, nanopores can be used to study the translocation of charged species, such as ions or nucleic acids. There are many promising applications for nanopores; for instance, developing a DNA sequencing technique or building nanofluidic devices. The Resource is working in close collaboration with experimentalists (Gregory Timp UIUC, Zuzanna Siwy UCI) and theoreticians (Jean-Pierre Leburton UIUC, Thorsten Ritz UCI) to understand the physics of synthetic nanopores and improve their usability as sensors. Atomic-scale modeling was carried out in the following directions: (i) translocating DNA-protein complexes through nanopores; (ii) stretching/unzipping DNA hairpins with a nanopore; (iii) sensing DNA sequence with a nanopore capacitor and (iv) modeling of ion interactions inside nanopores.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 纳米孔是带有纳米尺度半径的小孔。它们在大自然中发现 例如蛋白质α-丝素蛋白孔[1]，但也可以人为地是 在基于硅的膜[2]和聚合物晶片[3]中生产。浸入时 在水溶液和施加的电压中，可以使用纳米孔 研究带电物种的易位，例如离子或核酸。那里 是纳米孔的许多有前途的应用；例如，开发DNA 测序技术或构建纳米流体设备。资源正在工作 与实验者密切合作（Gregory Timp uiuc，Zuzanna Siwy UCI）和理论家（Jean-Pierre Leburton UIUC，Thorsten Ritz UCI）了解 合成纳米孔的物理学并提高其作为传感器的可用性。 原子级建模是在以下方向上进行的：（i）易位 DNA蛋白质复合物通过纳米孔； （ii）拉伸/解压缩DNA发夹 用纳米孔； （iii）用纳米孔电容器传感DNA序列和 （iv）纳米孔内部离子相互作用的建模。