INBRE: URI: CENTRALIZED RES CORE FACILITY

INBRE：URI：集中资源核心设施

• 批准号: 8167602
• 负责人: AFTAB J AHMED
• 金额: $ 17.31万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167602
• 关键词: Aerosols; Apoptosis; Biological Assay; Biological Markers; Biomedical Research; Cells; Charge; Chemicals; Collaborations; Communities; Computer Retrieval of Information on Scientific Projects Database; Consultations; Core Facility; Educational process of instructing; Educational workshop; Environment; Equipment; Faculty; Fees; Frequencies; Funding; Future; G Protein-Coupled Receptor Genes; Genes; Grant; Hand; Institution; Label; Laboratories; Manufacturer Name; Nucleic Acids; Participant; Pharmacy facility; Postdoctoral Fellow; Printing; Production; Reader; Recommendation; Research; Research Personnel; Research Project Grants; Research Training; Resources; Rhode Island; Safety; Services; Source; Students; System; Techniques; Technology; Time; Toxic effect; Toxicology; Training; Training Support; United States National Institutes of Health; Vision; base; college; cost; design; detector; experience; frontier; graduate student; instrument; instrumentation; migration; novel; posters; programs; small molecule; Aerosols; Apoptosis; Biological Assay; Biological Markers; Biomedical Research; Cells; Charge; Chemicals; Collaborations; Communities; Computer Retrieval of Information on Scientific Projects Database; Consultations; Core Facility; Educational process of instructing; Educational workshop; Environment; Equipment; Faculty; Fees; Frequencies; Funding; Future; G Protein-Coupled Receptor Genes; Genes; Grant; Hand; Institution; Label; Laboratories; Manufacturer Name; Nucleic Acids; Participant; Pharmacy facility; Postdoctoral Fellow; Printing; Production; Reader; Recommendation; Research; Research Personnel; Research Project Grants; Research Training; Resources; Rhode Island; Safety; Services; Source; Students; System; Techniques; Technology; Time; Toxic effect; Toxicology; Training; Training Support; United States National Institutes of Health; Vision; base; college; cost; design; detector; experience; frontier; graduate student; instrument; instrumentation; migration; novel; posters; programs; small molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The RI-INBRE Centralized Research Core Facility is in its seventh year of providing research and training support to the Network participants and the Rhode Island biomedical research community. During the current grant period more than 57 research groups have used the Facility, with an average quarterly cumulative frequency of 1,200 usages. The Facility users consisted of 32 faculty, 31 postdoctoral fellows, 93 graduate students, and 20 undergraduate students. The Facility staff provided training and consultation to all new users and in order to better utilize various technologies in their research projects. In addition, the staff continued to participate in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. These courses are designed to provide both theoretical and hands-on experience to students using the Facility's equipment. Throughout the year, several instructional seminars and training workshops were organized in collaboration with the instrument manufacturers. The topics included: "Discovery and characterization of novel biomarkers using SELDI-TOF technology"; "Xcelligence System a new frontier in label free, real time cell based assays for apoptosis, and GPCR invasion migration"; " QSTAR-MS/MS in a small molecule environment"; " Charged Aerosol Detector (CAD) a novel mass sensitive universal detector for the routine determination of any non-volatile chemical species; and "Profiling chemical toxicity using CDAS safety toxicology platform in the EPA ToxCast program". Based on the recommendations received from the RI-IBRE participants, new equipment acquired this year included the following: Quick Gene 810 nucleic acid isolation system; Polarstar Optima microplate reader' and Cellometer Vision automated cell counter. The SEDI-TOF system has become obsolete because BioRad has discontinued future production and upgrade. The Facility will be looking to replace this system with a new TOF-TOF machine with SELDI capability. A total of 170 posters were printed with non-RI-INBRE researchers paying a modest fee to cover the cost of consumables. The equipment and consultation were continued to be made available to the researchers at no cost. Overall, the usage of the Core Facility instrumentation and its consultative services has continued to increase.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 RI-INBRE集中研究核心设施在为网络参与者和罗德岛生物医学研究社区提供研究和培训支持的第七年。 在当前赠款期间，超过57个研究小组使用了该设施，平均季度累积频率为1200次使用。 设施用户包括32位教职员工，31名博士后研究员，93名研究生和20名本科生。 设施人员为所有新用户提供了培训和咨询，以便更好地利用其研究项目中的各种技术。 此外，工作人员继续参加药学学院提供的本科和研究生实验室课程的各种技术。 这些课程旨在使用设施的设备为学生提供理论和实践经验。 全年，与仪器制造商合作组织了几个教学研讨会和培训研讨会。主题包括：“使用Seldi-Tof Technology对新型生物标志物的发现和表征”； “ Xcelligence系统是无标签，基于实时细胞的细胞凋亡和GPCR入侵迁移的新领域”； “小分子环境中的QSTAR-MS/MS”；带电的气溶胶探测器（CAD）是一种新型的质量敏感通用探测器，用于常规确定任何非挥发性化学物种；以及“使用CDAS安全毒理学平台在EPA Toxcast计划中使用CDAS安全毒理学平台进行化学毒性”。根据从RI-IBRE的建议中获得的建议，今年获得了新的设备。自动化的式系统已经过时了，因为Biorad已停止生产和升级，该设施将用新的TOF-TOF机器替换了塞尔迪（Seldi）的塞尔德（Seldi）。设施工具及其咨询服务继续增加。