INBRE: URI: CENTRALIZED RES CORE FACILITY

INBRE：URI：集中资源核心设施

• 批准号: 8360066
• 负责人: AFTAB J AHMED
• 金额: $ 43.52万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360066
• 关键词: Behavioral Research; Biomedical Research; Biotechnology; Cell Count; Collaborations; Communities; Computer software; Consultations; Core Facility; Data; Detection; Educational process of instructing; Educational workshop; Equipment; Faculty; Fees; Frequencies; Funding; Grant; Graph; Hand; High Pressure Liquid Chromatography; Imagery; Individual; Laboratories; Manufacturer Name; National Center for Research Resources; Ointments; Participant; Performance; Pharmacy facility; Postdoctoral Fellow; Principal Investigator; Printing; Reader; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resources; Rhode Island; Schedule; Science; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Students; Techniques; Technology; Training; Training Support; Transfection; United States National Institutes of Health; Universities; Vision; Visit; college; cost; design; experience; fast protein liquid chromatography; graduate student; instructor; instrument; lectures; mass spectrometer; outreach; posters; protein purification

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The RI-INBRE Centralized Research Core Facility is in its eight year of providing research and training support to the Network participants and the Rhode Island biomedical research community. During the current grant period more than 58 research groups have used the Facility, with an average quarterly cumulative frequency of 1,320 usages. The Facility users consisted of 32 faculty, 38 postdoctoral fellows, 90 graduate students, and 25 undergraduate students. Facility staff provided individual training and consultation to all users in order to better utilize the available technologies in various research projects. The staff continued to participate in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. These courses are designed to provide both theoretical and hands-on experience to students using the Core Facility. In addition, hand-on instrument training workshops were organized in collaboration with the manufacturer's representative on automatic cell counting and transfection efficiency of GFP using Cellometer Vision and FLUOstar Optima multi-detection plate reader. The staff also conducted a two-day separation science workshop on the use of HPLC and FPLC for protein purification. Lectures, discussions and hands-on experience were provided to workshop participants. As part of the outreach initiative, the Facility staff visited RI-INBRE supported faculty and students at Salve Regina University and presented a seminar entitled "Technologies Available for Biomedical Research in the State of Rhode Island through RI-INBRE Centralized Research Core Facility". Also, students in a biotechnology course at Roger Williams University and the faculty instructor visited the Core Facility. The use of some of the key instruments in the field of biotechnology was demonstrated. New equipment acquired this year included the following: Shimadzu AXIMA Performance MALDI-TOF-MS/MS mass spectrometer, SpectraMax-M2 microplate reader, NanoDrop ND2000 UV/VIS spectrometer, and Thermo Multifuge centrifuge. Installation and training of MALDI-TOF-MS/MS is scheduled for April 2011. The software additions included: GraphPad Prism Scientific Graphing, and Canvas 12 Technical Illustration and Data Visualization. A total of 193 posters were printed, with only the non-RI-INBRE researchers paying a modest fee to cover the cost of consumables. The equipment and consultations were continued to be made available to the researchers at no cost and the usage has continued to increase.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 RI-INBRE 集中研究核心设施为网络参与者和罗德岛生物医学研究界提供研究和培训支持已有八年了。在当前资助期内，超过 58 个研究小组使用了该设施，平均每季度累计使用次数为 1,320 次。该设施的用户包括 32 名教职人员、38 名博士后研究员、90 名研究生和 25 名本科生。设施工作人员为所有用户提供单独的培训和咨询，以便更好地在各种研究项目中利用现有技术。工作人员继续参与药学院本科生和研究生实验室课程的各种技术教学。这些课程旨在为使用核心设施的学生提供理论和实践经验。此外，还与制造商代表合作组织了关于使用 Cellometer Vision 和 FLUOstar Optima 多重检测读板机进行自动细胞计数和 GFP 转染效率的仪器操作培训研讨会。工作人员还举办了为期两天的分离科学研讨会，介绍如何使用 HPLC 和 FPLC 进行蛋白质纯化。为研讨会参与者提供了讲座、讨论和实践经验。 作为外展活动的一部分，设施工作人员访问了 RI-INBRE，为萨尔维里贾纳大学的教职员工和学生提供支持，并举办了题为“通过 RI-INBRE 集中研究核心设施在罗德岛州进行生物医学研究的技术”的研讨会。此外，罗杰威廉姆斯大学生物技术课程的学生和教师还参观了核心设施。 展示了生物技术领域一些关键仪器的使用。今年采购的新设备包括：Shimadzu AXIMA Performance MALDI-TOF-MS/MS 质谱仪、SpectraMax-M2 酶标仪、NanoDrop ND2000 UV/VIS 光谱仪和 Thermo Multifuge 离心机。 MALDI-TOF-MS/MS 的安装和培训计划于 2011 年 4 月进行。新增的软件包括：GraphPad Prism 科学绘图、Canvas 12 技术插图和数据可视化。总共印刷了 193 张海报，只有非 RI-INBRE 研究人员支付了少量费用来支付消耗品的费用。设备和咨询继续免费向研究人员提供，并且使用量持续增加。