Plasmonic nanopores for trapping, controlled motion and sequencing of DNA

用于 DNA 捕获、控制运动和测序的等离激元纳米孔

基本信息

批准号：
9128456
负责人：
Aleksei Aksimentiev
金额：
$ 52.98万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2018-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9128456
关键词：
Base Sequence Biomedical Research Biosensing Techniques DNA DNA Damage DNA Sequence Detection Development Devices Diagnostic Dimensions Disease Experimental Models Genetic Goals Health Hot Spot Individual Label Light Lipid Bilayers Medical Methods Modeling Molecular Molecular Conformation Molecular Structure Motion Nanostructures Nucleotides Optics Procedures Proteins Radiation Scattering Raman Spectrum Analysis Reading Reagent Reporting Research Research Project Grants Research Proposals Science Screening for cancer Single-Stranded DNA Spottings Stretching Structure Surface System cost design drug development epigenetic variation genetic makeup human DNA sequencing innovation molecular dynamics multiplex detection nanofabrication nanometer nanoplasmonic nanopore novel novel strategies plasmonics predictive modeling programs research study single molecule solid state

项目摘要

DESCRIPTION (provided by applicant): This research project aims to combine the unique and powerful capabilities of two exciting, rapidly evolving fields, plasmonics and nanopores, for the analysis of single DNA molecules. More specifically, recent advances in nanoplasmonics will be utilized to enable label-free, single-molecule trapping and sequencing of DNA using nanopores. A novel type of synthetic nanostructure will be developed to strongly focus light to very high intensity in a nanometer-dimension spot where a solid-state nanopore is created. Through that spot, a DNA molecule will be translocated in a controlled way, allowing the detection of the sequence of the DNA fragments that are sequentially exposed to the intense optical fields of the plasmonic hot spot. The unique aspect of the program is the use of plasmonic tweezers to control DNA in solid-state nanopores. This novel approach to advancing DNA through the nanopore simultaneously enables DNA sequence detection through surface-enhanced Raman spectroscopy. Because locally confined plasmonic fields enhance Raman scattering many orders of magnitude and because of the direct relationship of Raman spectra to the underlying molecular structure, sequence detection will be possible directly, without any labeling. The project's team is a synergetic combination of experts in biomolecular modeling (UIUC), nanopore experiments (TU Delft) and plasmonic sensing (TU Delft). The specific aims of the projects are to (i) use a plasmonic field to trap DNA in solid-state nanopores, (ii) develop a method to transport DNA through plasmonic nanopores in discrete, ultimately single-nucleotide steps, and (iii) detect the nucleotide sequence of trapped and moving DNA molecules by means of Raman spectroscopy.

描述（由申请人提供）：该研究项目旨在结合两个令人兴奋的，快速发展的领域，血浆和纳米孔的独特功能，以分析单个DNA分子。更具体地说，使用纳米孔对DNA的最新进展将用于启用无标签，单分子捕获和测序。将开发一种新型的合成纳米结构，以在创建固态纳米孔的纳米尺寸斑点中将光线度强烈聚焦到很高的强度。通过该位置，将以控制的方式易位DNA分子，从而检测出依次暴露于等离子热点的强烈光场的DNA片段的序列。该程序的独特方面是使用等离子镊子控制固态纳米孔中的DNA。这种通过纳米孔推进DNA的新方法可以通过表面增强的拉曼光谱法实现DNA序列检测。由于局部限制的等离子场增强了拉曼散射的许多数量级，并且由于拉曼光谱与基础分子结构的直接关系，因此将直接进行序列检测，而无需任何标记。该项目的团队是生物分子建模专家（UIUC），纳米孔实验（TU DELFT）和等离子传感（TU DELFT）的协同组合。项目的具体目的是（i）使用等离子体场在固态纳米孔中捕获DNA，（ii）开发一种通过离散的，最终是单核苷酸步骤通过等离激氧纳米孔传输DNA的方法，并且（iii）通过RAMAN Spectrrsproscops的依赖性和移动DNA分子的核苷酸序列。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Modulation of Molecular Flux Using a Graphene Nanopore Capacitor.

DOI：
10.1021/acs.jpcb.6b10574
发表时间：
2017-04-20
期刊：
The journal of physical chemistry. B
影响因子：
0
作者：
Shankla M;Aksimentiev A
通讯作者：
Aksimentiev A

Self-Aligned Plasmonic Nanopores by Optically Controlled Dielectric Breakdown.

DOI：
10.1021/acs.nanolett.5b03239
发表时间：
2015-10-14
期刊：
Nano letters
影响因子：
10.8
作者：
Pud S;Verschueren D;Vukovic N;Plesa C;Jonsson MP;Dekker C
通讯作者：
Dekker C

Mechanical Trapping of DNA in a Double-Nanopore System.

DOI：
10.1021/acs.nanolett.6b04642
发表时间：
2016-12-14
期刊：
Nano letters
影响因子：
10.8
作者：
Pud S;Chao SH;Belkin M;Verschueren D;Huijben T;van Engelenburg C;Dekker C;Aksimentiev A
通讯作者：
Aksimentiev A

Temperature dependence of DNA translocations through solid-state nanopores.