RAP PHOSPHATASES

RAP磷酸酶

• 批准号: 8170606
• 负责人: Matthew B Neiditch
• 金额: $ 0.41万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170606
• 关键词: Bacillus subtilis; Bacteria; Binding; Biochemical Genetics; Cell Signaling Process; Cells; Competence; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA; Development; Family; Funding; Gene Expression; Genetic; Grant; Institution; Peptides; Phosphoric Monoester Hydrolases; Proteins; Research; Research Personnel; Resources; Roentgen Rays; Signal Transduction; Signal Transduction Pathway; Source; Structure; Time; United States National Institutes of Health; prevent; promoter; protein function; quorum sensing

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Quorum sensing is a bacterial cell-cell communication process that allows groups of bacteria to act in unison, synchronizing important phenotypic changes. One family of secreted quorum-sensing signals, called Phr pentapeptides, is imported into the cell where they inhibit the activity of proteins called Raps. To determine how Rap proteins function mechanistically, and how Rap functions are in turn regulated by Phr peptides, we are studying two subsets of Bacillus subtilis Rap proteins. One Rap protein subset negatively regulates sporulation in B. subtilis by dephosphorylating a central protein in the sporulation signal transduction pathway, the other subset of Rap proteins downregulates the development of genetic competence by preventing the master transcriptional regulator of early competence gene expression from binding to target DNA promoters. We have crystallized Rap proteins in complex with their target effector proteins, as well as with their regulatory Phr peptides. The X-ray crystal structures of these complexes, combined with biochemical and genetic studies, will reveal for the first time how Rap proteins function mechanistically and how Phr peptides regulate Rap protein activity.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 群体感应是一种细菌细胞间的通讯过程，允许细菌群一致行动，同步重要的表型变化。 一种被称为 Phr 五肽的分泌群体感应信号家族被导入细胞，抑制 Raps 蛋白质的活性。 为了确定 Rap 蛋白如何发挥作用，以及 Rap 功能又如何受 Phr 肽调节，我们正在研究枯草芽孢杆菌 Rap 蛋白的两个子集。 Rap 蛋白的一个子集通过使孢子形成信号转导途径中的中心蛋白去磷酸化来负向调节枯草芽孢杆菌的孢子形成，而 Rap 蛋白的另一个子集则通过阻止早期能力基因表达的主转录调节因子与靶标结合来下调遗传能力的发展DNA启动子。 我们已经结晶了 Rap 蛋白与其靶效应蛋白以及其调节性 Phr 肽的复合物。 这些复合物的 X 射线晶体结构，结合生化和遗传学研究，将首次揭示 Rap 蛋白如何发挥作用，以及 Phr 肽如何调节 Rap 蛋白活性。