RAP PHOSPHATASES

RAP磷酸酶

• 批准号: 8170606
• 负责人: Matthew B Neiditch
• 金额: $ 0.41万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170606
• 关键词: Bacillus subtilis; Bacteria; Binding; Biochemical Genetics; Cell Signaling Process; Cells; Competence; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA; Development; Family; Funding; Gene Expression; Genetic; Grant; Institution; Peptides; Phosphoric Monoester Hydrolases; Proteins; Research; Research Personnel; Resources; Roentgen Rays; Signal Transduction; Signal Transduction Pathway; Source; Structure; Time; United States National Institutes of Health; prevent; promoter; protein function; quorum sensing

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Quorum sensing is a bacterial cell-cell communication process that allows groups of bacteria to act in unison, synchronizing important phenotypic changes. One family of secreted quorum-sensing signals, called Phr pentapeptides, is imported into the cell where they inhibit the activity of proteins called Raps. To determine how Rap proteins function mechanistically, and how Rap functions are in turn regulated by Phr peptides, we are studying two subsets of Bacillus subtilis Rap proteins. One Rap protein subset negatively regulates sporulation in B. subtilis by dephosphorylating a central protein in the sporulation signal transduction pathway, the other subset of Rap proteins downregulates the development of genetic competence by preventing the master transcriptional regulator of early competence gene expression from binding to target DNA promoters. We have crystallized Rap proteins in complex with their target effector proteins, as well as with their regulatory Phr peptides. The X-ray crystal structures of these complexes, combined with biochemical and genetic studies, will reveal for the first time how Rap proteins function mechanistically and how Phr peptides regulate Rap protein activity.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 法群传感是一种细菌细胞 - 细胞通信过程，允许细菌组成一致，同步重要的表型变化。 一个被称为PHR五肽的分泌群体感应信号的家族被进口到细胞中，它们抑制了称为Rap的蛋白质的活性。 为了确定RAP蛋白的机械性能以及RAP功能如何受PHR肽的调节，我们正在研究两种枯草芽孢杆菌RAP蛋白的子集。 一个RAP蛋白子群通过在孢子型信号转导途径中脱磷酸化的中心蛋白来对枯草芽孢杆菌中的孢子产生负面调节，RAP蛋白的另一个子集通过防止与目标DNA启动者结合的早期能力基因表达的主转录基因表达的主转录调节剂来下调遗传能力的发展。 我们具有与其靶效应蛋白及其调节性PHR肽的复合物中结晶的RAP蛋白。 这些复合物的X射线晶体结构，结合生化和遗传研究，将首次揭示RAP蛋白如何机械地发挥作用以及PHR肽如何调节RAP蛋白活性。