IDENTIFYING CONDENSIN INTERACTING PROTEINS

鉴定凝缩蛋白相互作用蛋白

• 批准号: 8171408
• 负责人: KIRSTEN A HAGSTROM
• 金额: $ 0.47万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171408
• 关键词: ATP Hydrolysis; Binding; Caenorhabditis elegans; Cell Cycle; Cell Death; Cell division; Cells; Centromere; Chromosome Segregation; Chromosome Structures; Chromosomes; Complex; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; DNA; Ensure; Environmental Wind; Failure; Funding; Gene Expression Regulation; Genome; Grant; Health; Human; Institution; Lead; Learning; Malignant Neoplasms; Meiosis; Mitosis; Proteins; Proteomics; Research; Research Personnel; Resolution; Resources; Sister Chromatid; Source; United States National Institutes of Health; Work; condensin; daughter cell; genetic regulatory protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 在每个细胞分裂期间，基因组必须准确复制并隔离完整 将染色体套入每个子细胞。染色体隔离的失败有 对人类健康的严重后果，因为创造出不适当的细胞 染色体数可以导致细胞死亡，出生缺陷或癌症。我们的研究重点 冷凝素，保守的蛋白质，可通过组织来确保准确的染色体分离 减数分裂和有丝分裂过程中的染色体结构。冷凝素结合染色体，为 被认为利用ATP水解的能量将DNA变成大环。我们证明了C. 秀丽隐杆线虫具有两个冷凝蛋白配合物，具有重叠的组件和不同的功能 有丝分裂和基因调节。我们以前的工作还提出了冷凝蛋白功能 超越简单的压实，以促进姐妹染色单体分辨率和丝粒 组织。要了解有关冷凝剂的更多信息，我们现在正在采用功能性蛋白质组学 鉴定冷凝蛋白相互作用蛋白的方法。初步结果已经确定了新的 凝蛋白亚基，意外的亚基相互作用和调节蛋白 将冷凝蛋白功能与细胞周期联系起来的机制。