IDENTIFYING CONDENSIN INTERACTING PROTEINS

鉴定凝缩蛋白相互作用蛋白

基本信息

批准号：
7420745
负责人：
KIRSTEN A HAGSTROM
金额：
$ 0.29万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-20 至 2007-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7420745
关键词：
cell cycle chromosome movement chromosomes proteins

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。在每个细胞分裂过程中，基因组必须准确地复制并将一组完整的染色体隔离到每个子细胞中。染色体隔离的失败对人类健康产生了严重的后果，因为创建具有不适当染色体数的细胞会导致细胞死亡，出生缺陷或癌症。我们的研究重点是冷凝蛋白，保守的蛋白质，可通过在减数分裂和有丝分裂过程中组织染色体结构来确保准确的染色体分离。冷凝剂结合染色体，被认为使用ATP水解的能量将DNA变成大环。我们表明秀丽隐杆线虫具有两个冷凝蛋白复合物，具有重叠的成分和有丝分裂和基因调节的不同功能。我们以前的工作还表明，冷凝蛋白功能超出简单的压实功能，以促进姐妹染色体分辨率和丝粒组织。要了解有关冷凝蛋白的更多信息，我们现在正在采用一种功能性蛋白质组学方法来识别冷凝蛋白相互作用的蛋白质。初步结果已经确定了新的冷凝蛋白亚基，意外的亚基相互作用以及调节蛋白，这些蛋白指向将冷凝蛋白功能与细胞周期联系起来的机制。