IDENTIFYING CONDENSIN INTERACTING PROTEINS

鉴定凝缩蛋白相互作用蛋白

• 批准号: 8365868
• 负责人: KIRSTEN A HAGSTROM
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365868
• 关键词: ATP Hydrolysis; Binding; Biology; Caenorhabditis elegans; Cell Cycle; Cell Death; Cell division; Cells; Centromere; Chromosome Segregation; Chromosome Structures; Chromosomes; Complex; Congenital Abnormality; DNA; Ensure; Environmental Wind; Failure; Funding; Fungal Genome; Gene Expression Regulation; Genome; Grant; Health; Human; Lead; Learning; Malignant Neoplasms; Meiosis; Mitosis; National Center for Research Resources; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Resolution; Resources; Sister Chromatid; Source; United States National Institutes of Health; Work; condensin; cost; daughter cell; genetic regulatory protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 在每次细胞分裂期间，基因组必须准确复制并将一组完整的染色体分离到每个子细胞中。染色体分离失败会对人类健康产生严重后果，因为产生染色体数目不合适的细胞可能导致细胞死亡、出生缺陷或癌症。我们的研究重点是凝缩蛋白，即保守蛋白，它们通过在减数分裂和有丝分裂过程中组织染色体结构来确保准确的染色体分离。凝缩蛋白与染色体结合，被认为利用 ATP 水解的能量将 DNA 缠绕成大环。我们发现，秀丽隐杆线虫具有两个具有重叠成分和在有丝分裂和基因调控中不同功能的凝缩蛋白复合物。我们之前的工作还表明，凝缩蛋白的功能不仅仅是简单的压缩，还可以促进姐妹染色单体分辨率和着丝粒组织。为了了解更多关于凝缩蛋白的信息，我们现在正在采用功能蛋白质组学方法来识别凝缩蛋白相互作用蛋白。初步结果已经确定了新的凝缩蛋白亚基、意想不到的亚基相互作用以及调节蛋白，这些蛋白指出了将凝缩蛋白功能与细胞周期联系起来的机制。