IDENTIFYING CONDENSIN INTERACTING PROTEINS

鉴定凝缩蛋白相互作用蛋白

• 批准号: 8365868
• 负责人: KIRSTEN A HAGSTROM
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365868
• 关键词: ATP Hydrolysis; Binding; Biology; Caenorhabditis elegans; Cell Cycle; Cell Death; Cell division; Cells; Centromere; Chromosome Segregation; Chromosome Structures; Chromosomes; Complex; Congenital Abnormality; DNA; Ensure; Environmental Wind; Failure; Funding; Fungal Genome; Gene Expression Regulation; Genome; Grant; Health; Human; Lead; Learning; Malignant Neoplasms; Meiosis; Mitosis; National Center for Research Resources; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Resolution; Resources; Sister Chromatid; Source; United States National Institutes of Health; Work; condensin; cost; daughter cell; genetic regulatory protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在每个细胞分裂过程中，基因组必须准确地复制并将一组完整的染色体隔离到每个子细胞中。染色体隔离的失败对人类健康产生了严重的后果，因为创建具有不适当染色体数的细胞会导致细胞死亡，出生缺陷或癌症。我们的研究重点是冷凝蛋白，保守的蛋白质，可通过在减数分裂和有丝分裂过程中组织染色体结构来确保准确的染色体分离。冷凝剂结合染色体，被认为使用ATP水解的能量将DNA变成大环。我们表明秀丽隐杆线虫具有两个冷凝蛋白复合物，具有重叠的成分和有丝分裂和基因调节的不同功能。我们以前的工作还表明，冷凝蛋白功能超出简单的压实功能，以促进姐妹染色体分辨率和丝粒组织。要了解有关冷凝蛋白的更多信息，我们现在正在采用一种功能性蛋白质组学方法来识别冷凝蛋白相互作用的蛋白质。初步结果已经确定了新的冷凝蛋白亚基，意外的亚基相互作用以及调节蛋白，这些蛋白指向将冷凝蛋白功能与细胞周期联系起来的机制。