THERMO COMPARISON BETWEEN LTQ AND VELOS

LTQ 和 VELOS 之间的热比较

• 批准号: 8171367
• 负责人: VLAD ZABROUSKOV
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171367
• 关键词: Adopted; Caenorhabditis elegans; Complex; Computer Retrieval of Information on Scientific Projects Database; Detection; Electrospray Ionization; Funding; Grant; Institution; Ions; Liquid Chromatography; Penetration; Peptides; Proteins; Proteome; Proteomics; Research; Research Personnel; Resolution; Resources; Saccharomyces cerevisiae; Source; Time; United States National Institutes of Health; Width; base; design; improved; instrument; instrumentation; mass spectrometer; pressure; protein aminoacid sequence; research study; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The considerable progress in high-throughput proteomics analysis via liquid chromatography-electrospray ionization-tandem mass spectrometry over the past decade has been fueled to a large degree by continuous improvements in instrumentation. High-throughput identification experiments are based on peptide sequencing and are largely accomplished through the use of tandem mass spectrometry, with ion trap and trap-based instruments having become broadly adopted analytical platforms. To satisfy increasingly demanding requirements for depth of characterization and throughput, we present a newly developed dual-pressure linear ion trap mass spectrometer (LTQ Velos) that features increased sensitivity, afforded by a new source design, and demonstrates practical cycle times 2 times shorter than that of an LTQ XL, while improving or maintaining spectral quality for MS/MS fragmentation spectra. These improvements resulted in a substantial increase in the detection and identification of both proteins and unique peptides from the complex proteome of Caenorhabditis elegans, as compared to existing platforms. The greatly increased ion flux into the mass spectrometer in combination with improved isolation of low-abundance precursor ions resulted in increased detection of low-abundance peptides. These improvements cumulatively resulted in a substantially greater penetration into the baker's yeast (Saccharomyces cerevisiae) proteome compared to LTQ XL. Alternatively, faster cycle times on the new instrument allowed for higher throughput for a given depth of proteome analysis, with more peptides and proteins identified in 60 min using an LTQ Velos than in 180 min using an LTQ XL. When mass analysis was carried out with resolution in excess of 25 000 full width at half-maximum (fwhm), it became possible to isotopically resolve a small intact protein and its fragments, opening possibilities for top down experiments.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在过去十年中，通过液相色谱 - 电喷雾电离串联质谱法在高通量蛋白质组学分析中取得了很大的进步，这在很大程度上通过仪器的持续改进来促进。高通量识别实验基于肽测序，并在很大程度上通过使用串联质谱来完成，而基于离子陷阱和基于陷阱的仪器已广泛采用了分析平台。为了满足对表征和吞吐量深度越来越苛刻的要求，我们提出了新开发的双压线性离子陷阱质谱仪（LTQ VELOS），其具有更高的敏感性，由新的源设计提供，并在实际的周期设计中提供了2倍，比LTQ XL的较短的速度比LTQ XL的较短或维持频谱质量的spectraptra spectra spectra spectra spectra spectra spectra spectra。与现有平台相比，这些改进导致了秀丽隐杆线虫的复杂蛋白质组的检测和鉴定和鉴定蛋白质和独特的肽的大幅增加。从离子通量大大增加到质谱仪，结合改善了低丰度前体离子的分离，导致对低丰度肽的检测增加。与LTQ XL相比，这些改善累计导致了大大渗透到面包师酵母（酿酒酵母）的蛋白质组中。另外，新仪器上的循环时间更快，可以在给定的蛋白质组分析深度进行更高的吞吐量，使用LTQ丝线在60分钟内比使用LTQ XL在60分钟内鉴定出更多的肽和蛋白质。当质量分析以超过25 000的全宽度（FWHM）的分辨率超过25 000（FWHM）时，可以通过同位素解决一个小的完整蛋白质及其片段，从而开放了自上而下实验的可能性。