Molecular Regulators of Synaptic Specificity

突触特异性的分子调节剂

基本信息

批准号：
10581824
负责人：
Tyler J. Kennedy
金额：
$ 0.25万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-03-02 至 2023-08-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10581824
关键词：
Adopted Affect Afferent Neurons Animal Model Architecture Axon Behavior Behavioral Behavioral Assay Biological Assay Brain Caenorhabditis elegans Cells Cloning Complex Computer software Databases Defect Dendrites Development Electronic Medical Records and Genomics Network Environment Excision Gene Expression Genes Genetic Genetic Screening Genome Goals Individual Instinct Label Life Cycle Stages Link Locomotion Maintenance Mammals Maps Methods Modeling Molecular Molecular Analysis Molecular Cloning Morphogenesis Movement Mutagenesis Mutation Nervous system structure Neurodevelopmental Disorder Neurons Nociception Nociceptors Oranges Output Pathway interactions Pattern Phenotype Process Proteins RNA Interference Reproducibility Research Role Site Specificity Synapses Testing To specify Video Recording Work autism spectrum disorder behavior test design expectation genetic analysis grasp in vivo live cell imaging meetings mutant mutation screening nervous system disorder new therapeutic target novel optogenetics postsynaptic programs reconstitution response stem synaptogenesis tool transcription factor transcriptome sequencing

项目摘要

Project Summary Specific circuits in the brain determine how we sense and respond to our environment. These highly connected networks emerge during development as neurons extend projections to defined meeting sites, identify partners, and begin synaptogenesis. Although initial connections may be modified later, the overall pattern of connectivity is predictable thus suggesting that partner selection can be encoded by the genome. It follows that genetic analysis can be powerfully employed to find synaptic specificity genes by identifying mutants with altered patterns of connectivity. With the goal of identifying novel, conserved target selection proteins, I will screen for mutations that disrupt distinctive behaviors that depend on neuron-specific synapses in C. elegans. This work focuses on the PVD sensory neuron and its synaptic targets, PVC and AVA. PVD stimulation activates PVC, its dominant partner, and triggers forward movement. If the PVC connection is removed, however, AVA is activated instead, resulting in reverse locomotion. Thus, mutants that selectively disrupt either PVD→PVC or PVD→AVA connections can be identified from readily distinguished behaviors (e.g., forward vs reverse movement). With its short life cycle and powerful genetic tools, C. elegans is especially useful for unbiased genetic screens. In Aim 1 I will use an optogenetic strategy to activate PVD in a forward genetic EMS mutagenesis screen that uses a high-throughput video recording system (WormLab) to identify mutants with selectively altered locomotion. Behavioral mutants with these specific locomotory phenotypes will be screened with GRASP (GFP Reconstitution Across Synaptic Partners) markers to confirm that either PVD→PVC or PVD→AVA synapses are disrupted during synaptogenesis. Molecular cloning methods will be used to identify the affected synaptic specificity genes. Aim 2 adopts an independent approach that stems from the expectation that synaptic specificity genes in PVD should be regulated by cell autonomous transcription factors (TFs). My strategy exploits a list of 35 PVD-enriched TFs previously derived from RNA-Seq profiling. I will use RNAi and available genetic mutants in the GRASP marker assay to test each of these TFs for potential roles in either PVD→PVC or PVD→AVA synaptogenesis. This TF screen has the advantage of dysregulating multiple target genes simultaneously for a robust synaptic specificity phenotype. I will use PVD-specific RNA-Seq to identify the targets of the synapse-specific TFs and then test them individually for roles in PVD synaptic specificity using the behavioral assay and GRASP markers. Together, these approaches in C. elegans are expected to reveal key determinants of synaptic specificity that can be tested for conserved roles in more complex nervous systems and for links to neurological disorders associated with altered synaptogenesis such as Autism Spectrum Disorder (ASD).

项目摘要大脑中的特定电路决定了我们对这些高度联系的感觉随着神经元将预测扩展到定义的会议网站，确定合作伙伴，并开始突触发生。因此可以预测，因此表明伴侣的选择可以由基因组编码。可以通过鉴定具有替代品的突变体来强大地使用分析来找到突触特异性基因连通性。这种依赖于秀丽隐杆线的神经元特异性突触的独特行为。 PVD感觉神经元及其突触靶标PVC和AVA。合作伙伴，并触发前向运动。导致反向运动。可以从准备好的行为（例如，向后运动）中识别连接。秀丽隐杆线虫在短暂的遗传筛查中特别有用 1我将使用光遗传学策略在使用的前向遗传EMS Mutajenesis屏幕中激活PVD 高通量视频记录系统（Wormlab）以识别具有选择运动的突变体。具有特定运动表型的行为突变体将用掌握（GFP）筛选跨突触伙伴的重新建立）标记以确认PVD→PVC或PVD→AVA突触是突触发生过程中ers段。特异性基因2采用了一种独立的方法 PVD中的特异性基因应由细胞自主转录因子（TFS）定制从RNA-Seq分析得出的35个富含PVD的TFS的列表。 GRASP标记测定中的突变体测试每个TF在PVD→PVC或OR中的潜在作用 PVD→AVA突触发生。同时使用鲁棒的突触特异性表型。使用突触特异性TFS和测试它们在PVD突触特异性中的角色单独测试行为分析和抓握标记，预计秀丽隐杆线中的这些方法会揭示关键在更复杂的神经系统中，测试的突触特异性的决定因素以及与随着自闭症谱系障碍等突触发生变化相关的神经系统疾病的联系（ASD）。