STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSIS

拟南芥应激激素信号转导

• 批准号: 8171389
• 负责人: JULIAN I SCHROEDER
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171389
• 关键词: Abscisic Acid; Arabidopsis; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Droughts; Funding; Gene Expression; Genomics; Grant; Hormones; Institution; Lead; Mass Spectrum Analysis; Proteins; Research; Research Personnel; Resources; Signal Transduction; Source; Stress; United States National Institutes of Health; knockout gene; protein function; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The stress hormone abscisic acid is crucial to survival in Arabidopsis upon drought stress. Key abscisic acid signal transduction proteins have YFP fusions and have had complexes purified for analyzing signal transduction partners using mass spectrometry. This research will lead to identification of new candidate proteins that function in early abscisic acid signal transduction. The identified proteins will further be compared to guard cell specific genomic scale analyses of gene expression that have been completed using Affymetrix Genechips. Gene knockout experiments will verify the functions of the mass spectrometrically identified proteins.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 干旱胁迫下的拟南芥中的应力激素脱甲酸对于在拟南芥中存活至关重要。关键的脱甲酸信号转导蛋白具有YFP融合，并具有纯化的复合物，用于使用质谱分析信号转导伴侣。这项研究将导致鉴定出在早期脱离甲酸信号转导中起作用的新候选蛋白。将进一步比较已鉴定的蛋白质与使用Affymetrix Genechips完成的基因表达的防护细胞特异性基因组尺度分析。基因敲除实验将验证质谱鉴定的蛋白质的功能。