STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSIS

拟南芥应激激素信号转导

• 批准号: 8171389
• 负责人: JULIAN I SCHROEDER
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171389
• 关键词: Abscisic Acid; Arabidopsis; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Droughts; Funding; Gene Expression; Genomics; Grant; Hormones; Institution; Lead; Mass Spectrum Analysis; Proteins; Research; Research Personnel; Resources; Signal Transduction; Source; Stress; United States National Institutes of Health; knockout gene; protein function; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The stress hormone abscisic acid is crucial to survival in Arabidopsis upon drought stress. Key abscisic acid signal transduction proteins have YFP fusions and have had complexes purified for analyzing signal transduction partners using mass spectrometry. This research will lead to identification of new candidate proteins that function in early abscisic acid signal transduction. The identified proteins will further be compared to guard cell specific genomic scale analyses of gene expression that have been completed using Affymetrix Genechips. Gene knockout experiments will verify the functions of the mass spectrometrically identified proteins.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 应激激素脱落酸对于拟南芥在干旱胁迫下的生存至关重要。关键的脱落酸信号转导蛋白具有 YFP 融合体，并具有纯化的复合物，用于使用质谱分析信号转导伙伴。这项研究将导致鉴定在早期脱落酸信号转导中发挥作用的新候选蛋白。鉴定出的蛋白质将进一步与使用 Affymetrix Genechips 完成的保卫细胞特异性基因组规模的基因表达分析进行比较。基因敲除实验将验证质谱鉴定的蛋白质的功能。