STRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSIS

拟南芥应激激素信号转导

• 批准号: 8365799
• 负责人: JULIAN I SCHROEDER
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365799
• 关键词: Abscisic Acid; Arabidopsis; Biology; Cells; Droughts; Funding; Fungal Genome; Gene Expression; Genomics; Grant; Hormones; Lead; Mass Spectrum Analysis; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Signal Transduction; Source; Stress; United States National Institutes of Health; cost; knockout gene; protein complex; protein function; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The stress hormone abscisic acid is crucial to survival of Arabidopsis upon drought stress. Key abscisic acid signal transduction proteins have YFP fusions and will be used to purify protein complexes for analyzing signal transduction partners using mass spectrometry. This research will lead to identification of new further candidate proteins that function in early abscisic acid signal transduction. The identified proteins will further be compared to guard cell specific genomic scale analyses of gene expression that have been completed using Affymetrix Genechips. Gene knockout experiments will verify the functions of the mass spectrometrically identified proteins.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 应激激素脱甲酸对于干旱应激的拟南芥生存至关重要。关键的脱甲酸信号转导蛋白具有YFP融合，并将用于纯化蛋白质复合物，用于使用质谱法分析信号转导伴侣。这项研究将导致鉴定出在早期脱离酸性信号转导中起作用的新的进一步候选蛋白。将进一步比较已鉴定的蛋白质与使用Affymetrix Genechips完成的基因表达的防护细胞特异性基因组尺度分析。基因敲除实验将验证质谱鉴定的蛋白质的功能。