Structure and Function Analyses of DELLA Interacting Proteins in Arabidopsis

拟南芥中 DELLA 相互作用蛋白的结构和功能分析

• 批准号: 8372892
• 负责人: Tai-Ping Sun
• 金额: $ 28.85万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8372892
• 关键词: Abnormal Cell; Abscisic Acid; Acetylglucosamine; Amino Acids; Animals; Arabidopsis; Auxins; Binding; Biochemical; Biological Assay; C2H2 Zinc Finger; Cell Cycle; Cell Differentiation process; Cell Proliferation; Cells; Chromatin; Chromatin Structure; Collaborations; Complex; Cues; Defect; Development; Developmental Process; Disease; Diterpenes; Estradiol; Ethylenes; Eukaryota; Family; Gene Expression; Gene Targeting; Genes; Genetic; Genetic Epistasis; Gibberellins; Goals; Growth; Growth and Development function; Hair Cells; Hair Root; Histone Deacetylase; Hormones; Human; Individual; Institutes; Japan; Knowledge; Life Cycle Stages; Light; Link; Malignant Neoplasms; Maps; Mediating; Metabolic; Molecular; Nuclear; Nuclear Proteins; Nuclear Receptors; O-GlcNAc transferase; Pathway interactions; Pattern; Plant Growth Regulators; Plant Roots; Plants; Play; Polycomb; Proteins; Proteolysis; RNA Interference; Regulation; Regulator Genes; Reporter; Role; Seedling; Signal Pathway; Signal Transduction; Site; Sodium Chloride; Stress; Structure; System; Tertiary Protein Structure; Tobacco; Transcriptional Regulation; Transgenic Organisms; Ubiquitin; Yeasts; cell growth; chromatin modification; genetic regulatory protein; insight; jasmonic acid; multicatalytic endopeptidase complex; mutant; novel; pathogen; plant growth/development; programs; protein complex; protein protein interaction; receptor; response; transcription factor; tumorigenesis; yeast two hybrid system

DESCRIPTION (provided by applicant): The diterpenoid phytohormone gibberellin (GA) controls diverse developmental processes in plants. DELLA proteins are nuclear transcriptional regulators, which function as master growth repressors by inhibiting all aspects of GA responses. Binding of GA to its nuclear receptor GID1 (for GA INSENSITIVE DWARF1) enhances the GID1-DELLA interaction, which in turn leads to the rapid proteolysis of DELLA through the ubiquitin-proteasome pathway, and allows transcriptional reprogramming of GA-responsive genes. This GA- GID1-DELLA signaling module appears to control plant growth and development by integrating internal signals from other hormone pathways [auxin, abscisic acid, jasmonic acid (JA) and ethylene], and external biotic (pathogen) and abiotic cues (light conditions, cold and salt stresses). Recent studies in Arabidopsis show that DELLA directly binds to and regulates key transcription factors/regulators in several signaling pathways (light, JA and developmental pathways). Importantly, these DELLA interactors belong to three different families of transcription factors. However, the current knowledge on how DELLA functions to regulate plant growth is only the tip of the iceberg. The goals of this proposal are to elucidate novel functions of selected newly identified DELLA interactors in GA-modulated plant development by genetic and biochemical approaches, and to determine how DELLA binds to multiple classes of interacting proteins at the molecular level by structure analyses. In addition to transcription factors, some of the new DELLA interactors are chromatin modification proteins, or O-GlcNAc transferase (OGT). O-GlcNAcylation of target proteins by OGT regulates a myriad of cellular pathways in animals, but its function in plants is poorly understood. This study will shed light on how OGT regulates signaling pathways in plants. The proposed study on the DELLA and its interacting regulatory proteins will contribute significantly to the mechanism by which DELLA modulates plant growth. This study will also provide new insights into the mechanisms that modulate transcriptional reprogramming and chromatin structure during growth and development, which are a common theme in controlling cell differentiation and tumorigenesis in humans. PUBLIC HEALTH RELEVANCE: Abnormal cell proliferation of Cancer is often caused by defects in regulating proper gene expression. The proposed study will provide new insights into the regulatory mechanisms that control gene expression to manipulate cell growth and differentiation.

描述（由申请人提供）：二萜类植物激素赤霉素（GA）控制植物的多种发育过程。 DELLA 蛋白是核转录调节因子，通过抑制 GA 反应的各个方面发挥主要生长抑制因子的作用。 GA 与其核受体 GID1（GA 不敏感 DWARF1）的结合增强了 GID1-DELLA 相互作用，进而通过泛素-蛋白酶体途径导致 DELLA 快速蛋白水解，并允许 GA 响应基因的转录重编程。这种 GA-GID1-DELLA 信号模块似乎通过整合来自其他激素途径 [生长素、脱落酸、茉莉酸 (JA) 和乙烯] 的内部信号以及外部生物（病原体）和非生物信号（光照条件）来控制植物生长和发育。 、寒冷和盐胁迫）。最近对拟南芥的研究表明，DELLA 直接结合并调节多个信号通路（光、JA 和发育通路）中的关键转录因子/调节因子。重要的是，这些 DELLA 相互作用子属于三个不同的转录因子家族。然而，目前关于 DELLA 如何调节植物生长的知识只是冰山一角。该提案的目标是通过遗传和生化方法阐明新发现的 DELLA 相互作用蛋白在 GA 调节植物发育中的新功能，并通过结构分析确定 DELLA 如何在分子水平上与多种相互作用蛋白结合。除了转录因子之外，一些新的 DELLA 相互作用因子是染色质修饰蛋白，或 O-GlcNAc 转移酶 (OGT)。 OGT 对靶蛋白的 O-GlcNA 酰化可调节动物体内的多种细胞通路，但其在植物中的功能却知之甚少。这项研究将揭示 OGT 如何调节植物中的信号通路。拟议的关于 DELLA 及其相互作用的调节蛋白的研究将对 DELLA 调节植物生长的机制做出重大贡献。这项研究还将为生长和发育过程中调节转录重编程和染色质结构的机制提供新的见解，这是控制人类细胞分化和肿瘤发生的常见主题。 公共健康相关性：癌症细胞异常增殖通常是由调节适当基因表达的缺陷引起的。拟议的研究将为控制基因表达以操纵细胞生长和分化的调节机制提供新的见解。