Structure and Function Analyses of DELLA Interacting Proteins in Arabidopsis

拟南芥中 DELLA 相互作用蛋白的结构和功能分析

• 批准号: 8372892
• 负责人: Tai-Ping Sun
• 金额: $ 28.85万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8372892
• 关键词: Abnormal Cell; Abscisic Acid; Acetylglucosamine; Amino Acids; Animals; Arabidopsis; Auxins; Binding; Biochemical; Biological Assay; C2H2 Zinc Finger; Cell Cycle; Cell Differentiation process; Cell Proliferation; Cells; Chromatin; Chromatin Structure; Collaborations; Complex; Cues; Defect; Development; Developmental Process; Disease; Diterpenes; Estradiol; Ethylenes; Eukaryota; Family; Gene Expression; Gene Targeting; Genes; Genetic; Genetic Epistasis; Gibberellins; Goals; Growth; Growth and Development function; Hair Cells; Hair Root; Histone Deacetylase; Hormones; Human; Individual; Institutes; Japan; Knowledge; Life Cycle Stages; Light; Link; Malignant Neoplasms; Maps; Mediating; Metabolic; Molecular; Nuclear; Nuclear Proteins; Nuclear Receptors; O-GlcNAc transferase; Pathway interactions; Pattern; Plant Growth Regulators; Plant Roots; Plants; Play; Polycomb; Proteins; Proteolysis; RNA Interference; Regulation; Regulator Genes; Reporter; Role; Seedling; Signal Pathway; Signal Transduction; Site; Sodium Chloride; Stress; Structure; System; Tertiary Protein Structure; Tobacco; Transcriptional Regulation; Transgenic Organisms; Ubiquitin; Yeasts; cell growth; chromatin modification; genetic regulatory protein; insight; jasmonic acid; multicatalytic endopeptidase complex; mutant; novel; pathogen; plant growth/development; programs; protein complex; protein protein interaction; receptor; response; transcription factor; tumorigenesis; yeast two hybrid system

DESCRIPTION (provided by applicant): The diterpenoid phytohormone gibberellin (GA) controls diverse developmental processes in plants. DELLA proteins are nuclear transcriptional regulators, which function as master growth repressors by inhibiting all aspects of GA responses. Binding of GA to its nuclear receptor GID1 (for GA INSENSITIVE DWARF1) enhances the GID1-DELLA interaction, which in turn leads to the rapid proteolysis of DELLA through the ubiquitin-proteasome pathway, and allows transcriptional reprogramming of GA-responsive genes. This GA- GID1-DELLA signaling module appears to control plant growth and development by integrating internal signals from other hormone pathways [auxin, abscisic acid, jasmonic acid (JA) and ethylene], and external biotic (pathogen) and abiotic cues (light conditions, cold and salt stresses). Recent studies in Arabidopsis show that DELLA directly binds to and regulates key transcription factors/regulators in several signaling pathways (light, JA and developmental pathways). Importantly, these DELLA interactors belong to three different families of transcription factors. However, the current knowledge on how DELLA functions to regulate plant growth is only the tip of the iceberg. The goals of this proposal are to elucidate novel functions of selected newly identified DELLA interactors in GA-modulated plant development by genetic and biochemical approaches, and to determine how DELLA binds to multiple classes of interacting proteins at the molecular level by structure analyses. In addition to transcription factors, some of the new DELLA interactors are chromatin modification proteins, or O-GlcNAc transferase (OGT). O-GlcNAcylation of target proteins by OGT regulates a myriad of cellular pathways in animals, but its function in plants is poorly understood. This study will shed light on how OGT regulates signaling pathways in plants. The proposed study on the DELLA and its interacting regulatory proteins will contribute significantly to the mechanism by which DELLA modulates plant growth. This study will also provide new insights into the mechanisms that modulate transcriptional reprogramming and chromatin structure during growth and development, which are a common theme in controlling cell differentiation and tumorigenesis in humans. PUBLIC HEALTH RELEVANCE: Abnormal cell proliferation of Cancer is often caused by defects in regulating proper gene expression. The proposed study will provide new insights into the regulatory mechanisms that control gene expression to manipulate cell growth and differentiation.

描述（由申请人提供）：二萜类植物激素吉布雷林（GA）控制植物中的多种发育过程。 Della蛋白是核转录调节剂，它通过抑制GA反应的所有方面而充当主增长阻遏物。 GA与其核受体GID1的结合（对于Ga不敏感的矮人）增强了GID1-DELLA相互作用，这又导致通过泛素蛋白蛋白酶体途径对DELLA的快速蛋白解析，并允许对Ga-响应基因的转录重编程。该GA-GID1-DELLA信号传导模块似乎通过整合来自其他激素途径的内部信号（生长素，脱甲酸，茉莉丙酸（JA）和乙烯]，以及外部生物（病原体）和非生物线索（光，冷条件，冷条件，冷和盐应激）来控制植物的生长和发育。拟南芥的最新研究表明，Della直接与多种信号通路（光，JA和发育途径）中的关键转录因子/调节剂结合并调节。重要的是，这些Della相互作用者属于三个不同的转录因子家族。但是，目前关于Della如何调节植物生长的知识仅是冰山一角。该提案的目标是通过遗传和生化方法阐明选定的新鉴定的Della相互作用的新功能，并通过结构分析确定Della如何与分子水平上的多种相互作用蛋白结合。除转录因子外，一些新的DELLA相互作用者是染色质修饰蛋白或O-GLCNAC转移酶（OGT）。 OGT通过OGT对靶蛋白的O-Glcnacylation调节动物中无数的细胞途径，但其在植物中的功能知之甚少。这项研究将阐明OGT如何调节植物中的信号通路。提出的关于DELLA及其相互作用的调节蛋白的研究将对DELLA调节植物生长的机制产生重大贡献。这项研究还将提供有关调节转录重编程和染色质结构在生长和发育过程中的机制的新见解，这些机制是控制人类细胞分化和肿瘤发生的共同主题。 公共卫生相关性：癌症的异常细胞增殖通常是由调节适当基因表达的缺陷引起的。拟议的研究将提供有关控制基因表达以操纵细胞生长和分化的调节机制的新见解。