MOLECULAR CORE

分子核心

• 批准号: 8168495
• 负责人: Bharat Ramratnam
• 金额: $ 32.63万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168495
• 关键词: Arts; Basic Science; Biological Assay; Cell Line; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Detection; Equipment; Fee Schedules; Fee-for-Service Plans; Funding; Grant; Human; Immunoprecipitation; Individual; Institution; Instruction; Investigation; Laboratories; Mentors; Minority; Molecular; Molecular Biology; Mus; Northern Blotting; Nucleic Acids; Proteins; Publishing; Research; Research Personnel; Resources; Ribonucleases; Services; Source; Time; United States National Institutes of Health; Western Blotting; Work; base; cost; design; design and construction; research study; stem cell biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aims of the Molecular Core are to (1) To provide basic laboratory services to junior COBRE investigators in support of their stem cell biology centered basic science investigations, (2) To provide instruction, oversight and trouble shooting to junior investigators and their staff in the conduct of specific laboratory assays, (3) To assist in mentoring candidates identified by the Minority Core. The Core will maintain new laboratory space that has been created for the sole use of the Molecular Core. The laboratories are equipped with state-of-the art equipment sufficient for all experiments in molecular biology. The Core will offer support at no-cost to COBRE junior investigators defined as individuals who have not garnered independent R01-level support. The Core will offer assay support to recently funded COBRE investigators on a subsidized fee-for-service basis and non-COBRE investigators at a full-fee schedule. The Core will offer commonly used laboratory assays for the quantification and manipulation of nucleic acids (pcr primer design, Real Time PCR quantification, northern blot detection, ribonuclease protection assay, creation of nonviral/lentiviral, adenoviral expression constructs). The Core will maintain human and murine cell lines and their associated media. The Core will assist investigators in all aspects of protein manipulation including western blot, immunoprecipitation, creation of tagged (e.g His) expression constructs, design of SILAC experiments. The Core is staffed by individuals who have broad expertise in the above assays and who have published their work involving these assays.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 分子核心的具体目的是（1）向初级毛病研究人员提供基本的实验室服务，以支持其干细胞生物学以基础科学为中心的基础科学研究，（2），向初级调查人员及其员工提供指导，监督和麻烦进行特定的实验室分析，（3）协助指导少数核心核心确定的候选人。该核心将维护新的实验室空间，该空间是为分子芯唯一使用的。实验室配备了足以用于所有分子生物学实验的最先进的设备。该核心将在无成本的支持下为Cobre初级调查人员提供支持，该调查人员被定义为尚未获得独立R01级支持的个人。该核心将以补贴的服务基础为最近资助的COBRE调查人员提供测定支持，并按照全费计划提供非近代调查人员。核心将提供常用的实验室测定法，以定量和操纵核酸（PCR引物设计，实时PCR定量，北印迹检测，核糖核酸酶保护测定，创建非病毒/疾病病毒，腺病毒表达构建体）。核心将维护人类和鼠类细胞系及其相关培养基。核心将协助研究人员进行蛋白质操纵的各个方面，包括蛋白质印迹，免疫沉淀，标记（例如他的）表达构建体，SILAC实验的设计。核心是由在上述测定中具有广泛专业知识并发表其作品涉及这些测定法的个人组成的。