IMPAIRED ADIPOGENESIS IN INSULIN RESISTANCE: PILOT CLINICAL AMP IN VITRO STUDIES

胰岛素抵抗中脂肪生成受损：体外试验临床 AMP 研究

• 批准号: 8166981
• 负责人: RICHARD E PRATLEY
• 金额: $ 16.35万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166981
• 关键词: Address; Adipocytes; Adult; Age; Biopsy; Body fat; Buttocks; Clinical; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Diagnosis; Dual-Energy X-Ray Absorptiometry; Fatty acid glycerol esters; Funding; Gender; Grant; Growth; Hormones; In Vitro; Individual; Infusion procedures; Institution; Insulin; Insulin Resistance; Label; Laboratories; Lead; Measurement; Measures; Metabolism; Needles; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Patients; Radioactive; Recruitment Activity; Research; Research Personnel; Resources; Risk; Risk Factors; Sampling; Scanning; Series; Skin; Source; Testing; United States National Institutes of Health; Water; drinking; insulin sensitivity; lipid biosynthesis; new growth; oral glucose tolerance; prevent; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Obesity is a strong risk factor for developing type 2 diabetes (T2DM), but the reasons for this are not fully understood. In particular, it is not known why some obese people develop T2DM while other obese individuals do not. This study tests whether differences in fat cells (adipocytes) are to blame. Even in adults, fat cells are constantly being formed to replace old fat cells and to respond to the body's need to store excess energy. The ability to form new fat cells may be diminished in some individuals, leading to larger fat cells. These large fat cells secrete hormones that may increase risk for T2DM. This study tests whether fat cells from obese insulin resistant subjects (who are at risk for developing T2DM) form at a slower rate than those from insulin sensitive subjects (who are at lower risk for developing T2DM). To address this question we will recruit and study two groups of obese subjects, selected to be similar in age, gender and degree of obesity. One group of subjects will be obese and insulin resistant (the OIR group), while the other will be comparably obese, but insulin sensitive (OIS). Subjects will undergo a series of studies to characterize their metabolism including measurement of body fat by DXA scanning, oral glucose tolerance (a test used to diagnose diabetes) and measurement of insulin sensitivity in response to an infusion of insulin (a research study used to classify patients into the OIR and OIS groups). Small samples of fat (from just under the skin of the belly and the buttocks) will obtained using a needle on two occasions over 12 weeks. During these 12 weeks, subjects will drink a small amount of water that contains a non-radioactive label. This labeled water will allow us to measure the rate of growth of new fat cells in the body. We will also look at the rate of growth of fat cells obtained from these biopsies in the laboratory. The results of this study may tell us more about why certain obese people develop diabetes and why others do not. This might lead to new ways to prevent or treat T2DM.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 肥胖是患有2型糖尿病（T2DM）的强大危险因素，但是尚未完全了解其原因。特别是，尚不知道为什么有些肥胖者会发展T2DM而其他肥胖者则没有。这项研究测试了脂肪细胞（脂肪细胞）的差异是否应归咎于。即使在成年人中，也不断形成脂肪细胞来替代旧脂肪细胞并应对人体存储过多能量的需求。在某些个体中可能会降低形成新脂肪细胞的能力，从而导致较大的脂肪细胞。这些大脂肪细胞分泌激素，可能会增加T2DM的风险。这项研究测试了来自肥胖胰岛素耐药受试者（患有T2DM的风险）的脂肪细胞是否比胰岛素敏感受试者（患有T2DM的风险较低）的速度较慢。 为了解决这个问题，我们将招募和研究两组肥胖受试者，被选为年龄，性别和肥胖程度相似。一组受试者将是肥胖和胰岛素耐药性（OIR组），而另一组将是肥胖的，但胰岛素敏感（OIS）。受试者将进行一系列研究，以表征其代谢，包括通过DXA扫描测量体内脂肪，口服葡萄糖耐受性（用于诊断糖尿病的测试）以及响应胰岛素的胰岛素（一项用于将患者分类为OIR和OIS组）的胰岛素敏感性的测量）。较小的脂肪样本（从腹部的皮肤和臀部的皮肤下方）将在12周内两次获得针头。在这12周内，受试者将喝少量的水，其中含有非放射性标签。这种标记的水将使我们能够测量体内新脂肪细胞的生长速率。我们还将研究从实验室中这些活检中获得的脂肪细胞的生长速率。 这项研究的结果可能会告诉我们更多关于某些肥胖者为什么患糖尿病的原因以及其他人为什么不糖尿病的原因。 这可能会导致预防或治疗T2DM的新方法。