Function of Neural Activity in Developing Retina

神经活动在视网膜发育中的作用

基本信息

批准号：
8069152
负责人：
Marla Feller
金额：
$ 35.17万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-04-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8069152
关键词：
Action Potentials Address Adenosine Amacrine Cells Aminophylline Automobile Driving Axon Brain Calcium Cells Communication Congenital Abnormality Connexins Coupled Coupling Cyclic AMP Cyclic AMP-Dependent Protein Kinases Data Development Developmental Process Electrophysiology (science)Exhibits Eye Fire - disasters G-Protein-Coupled Receptors GABA-A Receptor Ganglion Cell Layer Gap Junctions Generations Genes Goals Human Development Image Injury Interneurons Knockout Mice Lateral Geniculate Body Life Mus Natural regeneration Nervous system structure Neurologic Neuromodulator Neurons Neurophysiology - biologic function Neurotransmitters Pattern Play Purinergic P1 Receptors Regulation Reporter Research Personnel Retina Retinal Retinal Ganglion Cells Reverse Transcriptase Polymerase Chain Reaction Role Second Messenger Systems Staging Structure System Thalamic structure Vision Visual Visual system structure Work base cholinergic connexin 36 gamma-Aminobutyric Acid in vivo insight neural circuit optical imaging postnatal programs relating to nervous system research study retinal neuron satisfaction second messenger superior colliculus Corpora quadrigemina

项目摘要

DESCRIPTION (provided by applicant): The mechanisms and function of spontaneous neural activity in the developing mammalian retina will be studied. Immature retinal neurons spontaneously generate correlated activity in the form of waves of action potentials that sweep across the retinal ganglion cell layer. These "retinal waves" occur during the developmental period when retinal ganglion cell axons are segregating into eye-specific layers in the lateral geniculate nucleus. Experiments using a combination optical imaging, single cell electrophysiology, and multielectrode array recordings are proposed to investigate the mechanisms of inter-cellular coupling that underlie the generation of retinal waves. First, using knockout mice, we will identify the role of a particular gap junction coupled network in generating the correlation structure of spontaneous action potentials in retinal ganglion cells. Second, we will study the mechanisms underlying the ability of the neuromodulators adenosine and GABA to powerfully modulate network activity. One result of this work will be to determine general organizations principles responsible for generating the activity patterns required for driving activity-dependent developmental processes. This work should further our understanding of the organizing principles that govern the normal development of the human nervous system, making it possible to understand the origin of neurological birth defects and to devise strategies that allow the nervous system to regenerate functioning neural circuits after injury.

描述（由申请人提供）：将研究发育中的哺乳动物视网膜自发神经活动的机制和功能。未成熟的视网膜神经元自发产生相关活性，以横穿视网膜神经节细胞层的作用电位的形式。这些“视网膜波”发生在视网膜神经节细胞轴突在横向遗传核中分离为眼睛特异性层时发生的。提出了使用组合光学成像，单细胞电生理学和多电极阵列记录的实验，以研究视网膜波产生的基础细胞间耦合的机理。首先，使用基因敲除小鼠，我们将确定特定间隙连接网络在产生视网膜神经节细胞中自发作用电位的相关结构中的作用。其次，我们将研究神经调节剂腺苷和GABA有效调节网络活动能力的机制。这项工作的结果将是确定负责产生驱动活动依赖活动发展过程所需的活动模式的普通组织原则。这项工作应进一步了解管理人类神经系统正常发展的组织原则，使您可以理解神经出生缺陷的起源，并制定策略，使神经系统受伤后能够再生功能的神经回路。