Exploring plasticity of the adult visual system using viral gene delivery

利用病毒基因传递探索成人视觉系统的可塑性

基本信息

批准号：
8035323
负责人：
MAUREEN E NEITZ
金额：
$ 37.07万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2012-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8035323
关键词：
Adult Age-Months Animals Appearance Applications Grants Area Behavior Behavior Control Biological Models Birth Blindness Canis familiaris Color Color Visions Data Development Discrimination Electroretinography Eye Felis catus Functional disorder Gene Delivery Genes Gerbils Goals Inherited Lateral Geniculate Body Leber&apos s amaurosis Light Literature Mediating Monitor Mutation Neuronal Plasticity Performance Photic Stimulation Photons Photoreceptors Phototransduction Pigments Plastics RPE65 protein Research Research Personnel Retina Retinal Retinal Cone Retinal Degeneration Saimiri Sensory Signal Transduction Sorting - Cell Movement Testing Therapeutic Intervention Viral Vision Visual Cortex Visual system structure Work behavior test blind critical period design functional loss gain of function gene therapy neglect object perception programs relating to nervous system research study retinal prosthesis viral gene delivery visual deprivation

项目摘要

DESCRIPTION (provided by applicant): The long-term goals of the work proposed in this grant application are to study neural plasticity of the adult mammalian visual system by adding new sensory input. Experiments that enhance rather than ablate sensory input offer a new avenue of research for answering fundamental questions about the plasticity of the adult visual system and have important implications for the rational design of gene therapy and retinal prostheses. We have chosen red-green color vision as a model system in which gain of function can be monitored quantitatively at the level of the retina using the electroretinogram and at the level of the visual cortex with behavioral tests of color vision. As a first step toward achieving our long term goals, we propose two specific aims. Specific Aim 1. To determine whether adding a third cone type via viral mediated delivery of a photopigment gene to the retina in a dichromatic adult squirrel monkey can expand his sensory capacity and change color vision behavior from dichromatic to trichromatic. Specific Aim 2. To determine whether expressing a long-wavelength sensitive pigment gene in a subset of UV cones via viral mediated gene delivery to the gerbil retina can expand the gerbil's color vision capacity to include making color discriminations in the red-green region of the spectrum.

描述（由申请人提供）：本赠款申请中提出的工作的长期目标是通过添加新的感官输入来研究成人哺乳动物视觉系统的神经可塑性。增强而不是消融感官输入的实验为回答有关成人视觉系统可塑性的基本问题提供了新的研究途径，并且对基因治疗和视网膜假体的合理设计具有重要意义。我们选择了红绿色的色觉作为模型系统，在该系统中，可以使用电视图和视觉效果测试在视网膜和视觉皮层的水平上定量监测功能的增益。作为实现长期目标的第一步，我们提出了两个具体目标。具体目标1。确定在二分法成年松鼠猴子中通过病毒介导的照相基因递送到视网膜的第三个锥类型是否可以扩大其感觉能力，并将色觉行为从二分法转变为三级体现。具体目的2。为了确定通过病毒介导的基因传递到沙鼠视网膜的紫外线中表达长波长敏感的色素基因，是否可以扩大沙鼠的色觉能力，包括在光谱的红绿色区域进行色彩区分。