CAN GENE THERAPY EXPAND SENSORY CAPACITY IN THE ADULT?

基因疗法可以扩展成人的感觉能力吗？

• 批准号: 8172785
• 负责人: MAUREEN E NEITZ
• 金额: $ 15.51万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172785
• 关键词: Adult; Animals; Behavior; Biological Models; Color Visions; Computer Retrieval of Information on Scientific Projects Database; Control Animal; Electroretinography; Funding; Genes; Goals; Grant; Institution; Mediating; Monitor; Neuronal Plasticity; Research; Research Personnel; Resources; Retina; Retinal Cone; Saimiri; Sensory; Source; Testing; United States National Institutes of Health; Viral; Visual Cortex; Visual system structure; Washington; Wisconsin; behavior test; design; gain of function; gene therapy; medical schools; research study; retinal prosthesis; subretinal injection

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long term goals of this project are to study neural plasticity of the adult mammalian visual system by adding new sensory input. Experiments that enhance rather than ablate sensory input offer a new avenue of research for answering fundamental questions about the plasticity of the adult visual system and have important implications for the rational design of gene therapy retinal prostheses. We have chosen red-green color vision as a model system in which gain of function can be monitored quantitatively at the level of the retina using the electroretinogram and at the level of the visual cortex with behavioral tests of color vision. Experiments for this project are designed to determine whether adding a third cone type via viral mediated delivery of a photopigment gene to the retina in a dichromatic adult squirrel monkey can expand his/her sensory capacity and change color vision behavior from dichromatic to trichromatic. Currently, animals that received subretinal injections and were tested using the electroretinogram at the Medical College of Wisconsin before moving to Washington in April of 2009 are undergoing daily behavioral tests of color vision. In addition, untreated control animals are also undergoing daily behavioral tests of color vision.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的长期目标是通过添加新的感觉输入来研究成人哺乳动物视觉系统的神经可塑性。 增强而不是消融感官输入的实验为回答有关成人视觉系统可塑性的基本问题提供了新的研究途径，并对基因治疗视网膜假体的合理设计具有重要意义。 我们选择了红绿色的色觉作为模型系统，在该系统中，可以使用电视图和视觉效果测试在视网膜和视觉皮层的水平上定量监测功能的增益。 该项目的实验旨在确定在二分型成年松鼠猴中通过病毒介导的光谱基因递送到视网膜的添加第三个锥类型是否可以扩大其感觉能力，并将彩色视觉行为从二分法转变为三级体系。 目前，接受视网膜下注射并在2009年4月移居华盛顿之前使用电子图的动物进行了测试。 此外，未经处理的对照动物也正在接受色觉的日常行为测试。