MoFlo XDP Cell Sorter

MoFlo XDP 细胞分选仪

• 批准号: 8052296
• 负责人: Marsha Wills-Karp
• 金额: $ 46.24万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-15 至 2012-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052296
• 关键词: Aging; Air; Asthma; Biliary Atresia; Cell Maintenance; Cell Separation; Cell surface; Cells; Chronic Myeloid Leukemia; Commit; Data; Dendritic Cells; Development; Disease; Doctor of Philosophy; Ensure; Flow Cytometry; Fostering; Funding; Future; Guanosine Triphosphate Phosphohydrolases; HIV; Hematopoietic; Hematopoietic stem cells; Hemophagocytic Lymphohistiocytoses; Housing; Immunology; Insulin-Dependent Diabetes Mellitus; Lasers; Lead; Maintenance; Medical center; Pathogenesis; Pediatric Hospitals; Population; Qualifying; Recovery; Regulatory T-Lymphocyte; Request for Proposals; Research; Research Personnel; Research Project Grants; Role; Running; Schedule; Services; Sorting - Cell Movement; T-Lymphocyte; Technology; Tumor Immunity; United States National Institutes of Health; Work; base; cancer stem cell; cost; instrument; interest; leukemia/lymphoma; meetings; member; novel; operation; population based

DESCRIPTION (provided by applicant): This proposal requests support for a MoFlo XDP Cell Sorter equipped with 3 lasers. This new instrument will be used to purify and sort rare cell populations based on their expression of unique cell surface markers using jet-in-air technology. Our request for the MoFlo XDP Cell Sorter is based on the following: (i) Our current Flow Cytometer Core is running above optimal capacity which is negatively impacting the tempo of our research; (ii) Several major users of the flow core require a jet- in-air sorter to make significant progress on their research and (iii) Our preliminary data demonstrate that the jet-in-air MoFlo Cell Sorter will meet both of these research needs. The instrument will support and enhance existing NIH-funded projects and will foster avenues of research that will likely lead to future funded projects. We will outline 8 NIH-funded investigators and their specific research projects that will be enhanced by the acquisition of the MoFlo XDP Cell Sorter. All of these investigators currently use the Cincinnati Children's Hospital Medical Center (CCHMC) Research Flow Cytometry Core (RFCC). Their research interests span a broad spectrum of biologic queries requiring the use of cell sorting, including: (1) mechanisms underlying the pathogenesis of asthma; (2) pathogenesis of biliary atresia; (3) the role of regulatory T cells in HIV and aging; (4) the role of cancer stem cells in chronic myelogenous leukemia; (5) mechanisms by which novel dendritic cell populations promote anti-tumor immunity; (6) mechanisms by which T cell effector function regulate hemophagocytic lymphohistiocytosis (HLH); (7) the role of GTPases in hematopoietic stem cell maintenance and development of hematopoietic diseases (leukemia, lymphoma); (8) dissecting the intercellular interplay that governs development of type I diabetes. Moreover, we list more than 20 additional investigators and describe how their NIH-funded research projects will benefit from this instrument acquisition. The PI, Dr. Wills-Karp, who is a leader in the field of immunology and a user of flow cytometry for over 20 years, will be responsible for the managerial oversight of the MoFlo XDP. She will work with the Director of the Research Flow Cytometry Core, Dr. Sherry Thornton, Ph.D. and the members of the Oversight Committee to establish scheduling priorities and cost- recovery rates. The MoFlo XDP sorter will be housed in the newly renovated CCHMC-RFCC. The Core Director will oversee the daily operation and maintenance of the Cell Sorter with the assistance of a highly qualified Core Manager and 3 technicians. CCHMC is highly committed to supporting its research cores and will support the long-term upkeep and maintenance of this instrument to ensure quality services are provided in a timely manner.

描述（由申请人提供）：该提案请求支持配备3个激光器的MOFLO XDP单元格。该新仪器将根据使用喷射机技术的独特细胞表面标记表达纯化和对稀有细胞群体进行净化和分类。我们对MOFLO XDP细胞分系程序的要求基于以下基础：（i）我们当前的流式细胞仪芯的运行量高于最佳容量，这对我们的研究的节奏产生了负面影响； （ii）流核的几个主要用户需要一个喷气空间分隔器，以在其研究上取得重大进展，并且（iii）我们的初步数据表明，喷射空中MOFLO细胞分系程序将满足这两个研究需求。该工具将支持并增强现有的NIH资助项目，并将促进研究途径，这可能会导致未来的资助项目。我们将概述8名NIH资助的研究人员及其特定的研究项目，这些项目将通过获取MOFLO XDP细胞分系台增强。所有这些研究人员目前都使用辛辛那提儿童医院医疗中心（CCHMC）研究流式细胞仪核心（RFCC）。他们的研究兴趣跨越了需要使用细胞分选的各种生物学查询，包括：（1）哮喘发病机理的基础机制； （2）胆道闭锁的发病机理； （3）调节性T细胞在HIV和衰老中的作用； （4）癌症干细胞在慢性粒细胞性白血病中的作用； （5）新型树突细胞群体促进抗肿瘤免疫的机制； （6）T细胞效应功能调节胞型淋巴虫组织细胞增多症（HLH）的机制； （7）GTPases在造血细胞维持和造血疾病的发育中的作用（白血病，淋巴瘤）； （8）解剖控制I型糖尿病发展的细胞间相互作用。此外，我们列出了另外20多名调查人员，并描述了其NIH资助的研究项目将如何从此工具中受益。 PI，Wills-Karp博士是免疫学领域的领导者，也是流式细胞仪的使用者已有20多年了，他将负责对Moflo XDP的管理监督。她将与研究流式细胞仪核心主任Sherry Thornton博士合作。监督委员会成员建立计划优先级和成本回收率。 MOFLO XDP分类器将安置在新近翻新的CCHMC-RFCC中。核心主管将在一位高素质的核心经理和3位技术人员的协助下监督细胞分隔器的日常操作和维护。 CCHMC高度致力于支持其研究核心，并将支持该工具的长期维护和维护，以确保及时提供优质的服务。