Multi-Photon Confocal Microscope

多光子共焦显微镜

• 批准号: 7839902
• 负责人: YI ZHENG
• 金额: $ 82.66万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-09 至 2012-05-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7839902
• 关键词: Animals; Cells; Cellular Structures; Child; Childhood; Communities; Contract Services; Development; Employment; Faculty; Financial Support; Foundations; Funding; Funding Mechanisms; Generations; Hematologist; Housing; Image; Institutes; Lasers; Life; Malignant Neoplasms; Microscope; Minor; Molecular; Mus; Neurologist; Occupations; Participant; Research; Research Personnel; Scanning; System; United States National Institutes of Health; Work; instrument; multi-photon; operation; programs

DESCRIPTION (provided by applicant): We propose to acquire a multi-photon ZEISS LSM710 laser confocal scanning microscope to image live cellular structures and molecular associations in live mice, for Cincinnati Children's Research Foundation (CCRF). CCRF has a strong community of developmental biologists, hematologists, neurobiologists, biochemists and molecular biologists, as well as pediatric clinicians, whose research programs will benefit enormously by the availability of this multi-photon confocal microscope. The participants included in this application reflect the diversity of the research programs and systems studied at CHRF, including hematologists, developmental biologist, neurologist, and cancer biologist, and encompass both senior and junior investigators. This application also reflects the genuine commitment on the part of the institute administration and the faculty to assist a new generation of biomedical researchers in the development of their research programs. A team of seven accomplished researchers will be the major users of the requested instrument, whose NIH funded studies would otherwise be unable to accomplish without such an instrument. This instrument will be a part of an integrated imaging research center, where instrument will be housed and fully supported by a core manager and technical staff in a small animal bio-bubble. In addition to major users, there will be opportunity for the instrument to be used by minor users/ new Investigators for live cell/live animal imaging studies. Towards this end we have obtained strong institutional commitments, in the form of financial support to cover operation shortfalls, service contract, and consumable supplies. The HEI funding mechanism will create new jobs for the instrument maker, ZEISS, in US, and will certainly retain and create employment of the technical support staff in Cincinnati. The instrument promises to be environmentally-friendly in many features and is best suited for the group of users whose NIH supported work will benefit tremendously by the added values.

描述（由申请人提供）：我们建议为辛辛那提儿童研究基金会（CCRF）购买一台多光子蔡司 LSM710 激光共焦扫描显微镜，用于对活体小鼠的活细胞结构和分子关联进行成像。 CCRF 拥有一个由发育生物学家、血液学家、神经生物学家、生物化学家和分子生物学家以及儿科临床医生组成的强大社区，他们的研究项目将因这种多光子共聚焦显微镜的可用性而受益匪浅。本申请中包含的参与者反映了 CHRF 研究项目和系统的多样性，包括血液学家、发育生物学家、神经学家和癌症生物学家，并且包括高级和初级研究人员。该申请还反映了研究所管理部门和教员对协助新一代生物医学研究人员开发研究项目的真诚承诺。由七名有成就的研究人员组成的团队将成为所需仪器的主要用户，如果没有这样的仪器，他们的 NIH 资助的研究将无法完成。该仪器将成为综合成像研究中心的一部分，该中心将安置在小动物生物泡中，并由核心管理人员和技术人员提供全面支持。除了主要用户外，次要用户/新研究人员也将有机会使用该仪器进行活细胞/活体动物成像研究。为此，我们获得了强有力的制度承诺，以财政支持的形式弥补运营缺口、服务合同和消耗品供应。 HEI资助机制将为美国仪器制造商蔡司创造新的就业机会，也必将保留和创造辛辛那提技术支持人员的就业机会。该仪器承诺在许多功能上都是环保的，最适合那些 NIH 支持的工作将从附加值中受益匪浅的用户群体。

项目成果

ETS transcription factors Etv2 and Fli1b are required for tumor angiogenesis.

• DOI: 10.1007/s10456-017-9539-8
• 发表时间: 2017-08
• 期刊: Angiogenesis
• 影响因子: 9.8
• 作者: Baltrunaite K;Craig MP;Palencia Desai S;Chaturvedi P;Pandey RN;Hegde RS;Sumanas S
• 通讯作者: Sumanas S