OCT Imaging Technology for Developmental Cardiology Research

用于发育心脏病学研究的 OCT 成像技术

• 批准号: 7472295
• 负责人: ANDREW Martin ROLLINS
• 金额: $ 37.14万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7472295
• 关键词: Algorithms; Animal Model; Arts; Biological; Birefringence; Blood; Cardiac; Cardiology; Cessation of life; Chick Embryo; Chickens; Congenital Disorders; Congenital Heart Defects; Data; Defect; Depth; Development; Diagnostic; Dyes; EFRAC; Echocardiography; Electrocardiogram; Embryo; Embryonic Heart; Emerging Technologies; Event; Fluorescence; Four-dimensional; Frequencies; Goals; Heart; Heart Diseases; Image; Image Analysis; Imagery; Imaging Techniques; Imaging technology; In Situ; Investigation; Knock-out; Life; Maps; Measurement; Measures; Mechanics; Methods; Morbidity - disease rate; Morphogenesis; Morphology; Motion; Mus; Noise; Optical Coherence Tomography; Optics; Pattern; Physiological; Preparation; Property; Purpose; Research; Research Personnel; Resolution; Sampling; Side; Signal Transduction; Speed; Staging; Standards of Weights and Measures; Stroke Volume; Structure; System; Techniques; Technology; Thick; Three-Dimensional Image; Three-Dimensional Imaging; Time; Tissue Sample; Tissues; Tubular formation; United States; Validation; Ventricular; base; congenital heart disorder; fetal; heart imaging; hemodynamics; improved; in utero; in vivo; insight; mortality; mouse model; novel; research study; technology development; three dimensional structure; tomography; tool; voltage

DESCRIPTION (provided by applicant): Heart disease remains the leading cause of morbidity and mortality in the United States, responsible for over 1,400,000 deaths in 2001. Limitations in technology have hampered our ability to ameliorate the impact of an important group of cardiac disorders, congenital heart disease. The multi-disciplinary project described in this five-year proposal will develop and validate new investigative and diagnostic technologies based on the emerging imaging technique, optical coherence tomography (OCT). OCT is capable of simultaneous imaging of structure and function in small biological samples with micrometer-scale resolution in vivo and in real time, and is potentially capable of measuring electromechanical events. A multi-disciplinary approach is necessary to target the development of these new tools for the purpose of improving our understanding of the mechanisms of congenital heart disease. Specific Aim 1: Develop and demonstrate OCT imaging technologies for developmental cardiology research applications and disseminate to collaborators for validation and baseline studies. Specific Aim 2: Develop and demonstrate image analysis and visualization methods and disseminate to collaborators for validation and baseline studies. Specific Aim 3: Validate and apply OCT technologies to investigations of normal and abnormal cardiac morphogenesis in the chick embryo. Longitudinal analysis. Specific Aim 4: Validate and apply OCT technologies to screen and analyze cardiac defects in genetically altered mouse lines. Analysis of conotruncal and ventricular wall abnormalities. The goal of this project is to develop and validate a novel set of tools that will combine structural and physiologic information at heretofore unachievable resolution scales for better insight into the mechanisms of normal and abnormal cardiac morphogenesis. The utility of these tools to enable experiments that were previously not possible will be established by baseline studies of chick and mouse models of normal and abnormal cardiac morphogenesis.

描述（由申请人提供）：心脏病仍然是美国发病率和死亡率的主要原因，2001 年导致超过 1,400,000 人死亡。技术的限制阻碍了我们改善先天性心脏病这一重要心脏病组影响的能力。心脏病。这项为期五年的提案中描述的多学科项目将开发和验证基于新兴成像技术光学相干断层扫描（OCT）的新研究和诊断技术。 OCT 能够以微米级分辨率在体内实时对小型生物样品的结构和功能进行同步成像，并且有可能测量机电事件。有必要采取多学科方法来开发这些新工具，以提高我们对先天性心脏病机制的理解。具体目标 1：开发和演示用于发育心脏病学研究应用的 OCT 成像技术，并向合作者传播以进行验证和基线研究。具体目标 2：开发和演示图像分析和可视化方法，并向合作者传播以进行验证和基线研究。具体目标 3：验证 OCT 技术并将其应用于鸡胚正常和异常心脏形态发生的研究。纵向分析。具体目标 4：验证并应用 OCT 技术来筛查和分析转基因小鼠品系的心脏缺陷。圆锥干和心室壁异常分析。该项目的目标是开发和验证一套新颖的工具，以迄今为止无法实现的分辨率尺度结合结构和生理信息，以便更好地了解正常和异常心脏形态发生的机制。这些工具的实用性将通过对正常和异常心脏形态发生的雏鸡和小鼠模型的基线研究来实现以前不可能进行的实验。