Resource Center for Stable Isotope-Resolved Metabolomics

稳定同位素解析代谢组学资源中心

基本信息

批准号：
8732640
负责人：
Teresa Whei-Mei Fan
金额：
$ 200.13万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-11 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8732640
关键词：
Address Adoption Algorithms Animal Model Animals Arts Award Biochemical Pathway Biomedical Research Cardiovascular Diagnostic Techniques Cell Culture Techniques Cells Client Clinical Research Communities Consultations Data Data Analyses Detection Development Devices Diabetes Mellitus Disease Distance Learning Education and Outreach Educational workshop Ensure Experimental Designs Fees Foundations Fractionation Freezing Fresh Tissue Funding Goals Grant Homeostasis Human Human Genome Informatics Infusion procedures Instruction International Internet Joints Laboratories Laboratory Research Lead Leadership Learning Malignant Neoplasms Mass Spectrum Analysis Metabolism Methodology Microfluidics Modeling Neurodegenerative Disorders Outcome Paper Pathway interactions Performance Preparation Procedures Process Proteome Publications Publishing Qualifying Regulation Research Research Personnel Resected Resource Informatics Resources Sampling Services Slice Solid Structure System Techniques Technology Therapeutic Tissues Tracer Training Training and Education Transplantation United States National Institutes of Health Universities Work Xenograft procedure base bench to bedside computerized data processing cost data acquisition design disease diagnosis experience handbook human subject instrumentation meetings metabolomics network models new therapeutic target operation outreach prevent programs software development stable isotope symposium tandem mass spectrometry therapeutic development ultra high resolution

项目摘要

DESCRIPTION: The human metabolome represents the functional display of the human genome and proteome. The regulation of its homeostasis is highly sensitive to both intrinsic and external factors, the perturbation of which can lead to disease development. Advances in mass spectrometry (MS), NMR, informatics, and sample processing have made possible large-scale interrogation of the human metabolome ("metabolomics") and is being increasingly applied to a variety of diseases, such as cancer, diabetes, neurodegenerative and cardiovascular disorders, for diagnosis and therapeutic purposes. The existing Center for Regulatory and Environmental Analytical Metabolomics (CREAM) conducts training and provides advice and full analytical resources, from sample processing, MS and NMR instrumentation, to informatics, which match with the major goals of the Common Fund. Moreover, CREAM is now in its 6th year of self- sufficiency through client fees and conducts translational clinical research, both of which are critical fifth-year exit goals of the Common Fund. Building on this solid foundation with added resources, the proposed Common Fund Regional Comprehensive Metabolomics Resource Core (RCMRC) at the University of Louisville will greatly expand CREAM's capabilities in sample processing, analytical/informatics developments and services, and outreach efforts under the leadership of the PD and Pis, who collectively have more than 95 years of experience in metabolomics. The Overall Goals of the RCMRC-CREAM are to facilitate and promote metabolomics research with an emphasis on stable isotope tracing for pathway elucidation in studies ranging from laboratory bench cell cultures, model animals, to human subjects, and to provide the state-of-art analytical and informatics resources, education, and training for the biomedical research community. These goals will be achieved via the following specific aims: SA1: To administer comprehensive Metabolomics resources for bench to bedside research; SA2: To develop and implement integrated Metabolomics platforms to enhance performance, sample & information throughput, and scientific relevance; SA3: To provide outreach and education of the wider community in Metabolomics approaches.

描述：人类代谢组代表人类基因组和蛋白质组的功能展示。其体内平衡的调节对内在和外在因素高度敏感，这些因素的扰动可能导致疾病的发展。质谱 (MS)、核磁共振、信息学和样品处理的进步使得对人类代谢组（“代谢组学”）的大规模研究成为可能，并且越来越多地应用于多种疾病，如癌症、糖尿病、神经退行性疾病和心血管疾病，用于诊断和治疗目的。现有的监管和环境分析代谢组学中心 (CREAM) 开展培训并提供建议和完整的分析资源，从样品处理、MS 和 NMR 仪器到信息学，这与共同基金的主要目标相匹配。此外，CREAM 目前已进入通过客户费用实现自给自足的第六个年头，并开展转化性临床研究，这两者都是共同基金第五年退出的关键目标。在此坚实的基础上，增加资源，拟议的路易斯维尔大学共同基金区域综合代谢组学资源核心 (RCMRC) 将极大扩展 CREAM 在样品处理、分析/信息学开发和服务以及在委员会领导下的外展工作的能力。 PD 和 Pis 在代谢组学领域总共拥有超过 95 年的经验。 RCMRC-CREAM 的总体目标是促进和促进代谢组学研究，重点是稳定同位素追踪，以阐明实验室台式细胞培养、模型动物和人类受试者等研究中的途径，并提供最先进的技术为生物医学研究界提供分析和信息学资源、教育和培训。这些目标将通过以下具体目标来实现： SA1：管理用于实验室到临床研究的全面代谢组学资源； SA2：开发和实施集成代谢组学平台，以提高性能、样本和信息吞吐量以及科学相关性； SA3：为更广泛的社区提供代谢组学方法的推广和教育。