FLOW CYTOMETRY SHARED RESOURCE

流式细胞术共享资源

• 批准号: 8113364
• 负责人: Steven A Porcelli
• 金额: $ 17.67万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8113364
• 关键词: Albert Einstein Cancer Center; Arts; Cancer Center; Cell Separation; Chemicals; Color; Computer Workstations; Computer software; Data; Dyes; Equipment; Faculty; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence; Fluorescence-Activated Cell Sorting; Fluorochrome; Goals; Hour; Label; Laboratories; Lasers; Libraries; Postdoctoral Fellow; Principal Investigator; Process; Proteins; Reagent; Research; Research Personnel; Resource Sharing; Schedule; Services; Site; Speed; Students; Technical Expertise; Techniques; Technology; Training; antibody conjugate; base; fluorescence microscope; instrument; instrumentation; member; new technology; population based; programs; research study

The Flow Cytometry Shared Resource provides AECC investigators with convenient and affordable access to a broad spectrum of important technologies in flow cytometry, and supports numerous research studies that rely on high speed fluorescence activated cell sorting (FACS) and advanced multiparameter fluorescence analysis. In addition to providing advanced flow cytometry equipment, the shared resource provides technical expertise and training to guide AECC investigators and their staff in the application of basic and advanced flow cytometry techniques. The facility also organizes and supports a program of seminars on new technologies that inform AECC investigators about newly emerging applications of FACS that can enhance their research. The major usage of this shared resource is for multiparameter analysis and high speed sorting of cell populations based on labeling with fluorochrome-conjugated antibodies or other fluorescent protein or chemical dye reagents. Training of many investigators in the use of the facility's analytical instruments has led to a very large and well-trained user base. During 2005-2006, the facility provided services to more than 275 registered users, consisting of faculty, postdoctoral fellows, students and technicians from 78 different laboratories. AECC members were principal investigators of 45 of these laboratories, and two thirds of total hours of usage of all facility services in 2005-2006 was attributable to the laboratories of AECC investigators. These investigators have access around the clock and seven days per week to the facility's analyzers, which include Becton-Dickinson FACScan (one laser, 3-color), FACSCalibur (two laser, 4-color), and LSR-II (4 laser, 12 color) instruments. Standard fluorescent microscopes and computer work stations with an extensive software library for processing of FACS data are also routinely available. High speed cell sorting with the facility's 3-laser, 8-color Dako-Cytomation MoFlo sorter is performed exclusively by the trained facility staff during regularly scheduled weekday hours. This shared resource has numerous value-added features, and receives substantial institutional support that has greatly assisted the acquisition of state-of-the-art instrumentation and a superb staff of flow cytometry experts.

流式细胞仪共享资源为AECC调查人员提供了方便且负担得起的访问 在流式细胞仪中广泛的重要技术，并支持众多研究 依靠高速荧光激活的细胞分选（FACS）和高级多参数 荧光分析。除了提供高级流式细胞仪设备外，共享资源 提供技术专业知识和培训，以指导AECC调查人员及其员工应用 基本和先进的流式细胞仪技术。该设施还组织并支持 有关新技术的研讨会，这些研讨会告知AECC调查人员FACS的新兴应用 这可以增强他们的研究。该共享资源的主要用途是用于多参数分析和 基于用荧光聚糖偶联的抗体或其他标记的细胞群体的高速分类 荧光蛋白或化学染料试剂。培训许多调查人员在使用该设施的 分析仪器导致了一个非常庞大且训练有素的用户群。在2005-2006期间，该设施 为275位注册用户提供服务，包括教职员工，博士后研究员，学生和 来自78个不同实验室的技术人员。 AECC成员是其中45个的主要调查员 实验室，以及2005 - 2006年所有设施服务总小时的三分之二。 AECC调查人员的实验室。 These investigators have access around the clock and seven days per 该设施分析仪的一周，其中包括Becton-Dickinson Facscan（一个激光，三色），FACSCALIBUR （两个激光，4色）和LSR-II（4个激光，12颜色）仪器。标准荧光显微镜和 具有用于处理FACS数据的广泛软件库的计算机工作站也常规 可用的。使用该设施的3射线，8色Dako-cytomation Moflo Sorter的高速单元格进行分类 在定期安排的工作日期间，由训练有素的设施人员专门执行。这共享 资源具有许多增值功能，并获得了大量的机构支持 协助收购了最先进的仪器和流式细胞仪专家的出色人员。