STRUCTURAL BIOLOGY SHARED RESOURCE

结构生物学共享资源

• 批准号: 7506865
• 负责人: STEVEN C. ALMO
• 金额: $ 10.04万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-25 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7506865
• 关键词: Albert Einstein Cancer Center; Anodes; Arts; Biochemical; Biological; Biology; Cancer Center; Cell-Free System; Cells; Charge; Complement; Computational Biology; Cryoelectron Microscopy; Crystallization; Custom; Data; Data Collection; Decompression Sickness; Development; Devices; Electron Spin Resonance Spectroscopy; Ensure; Environment; Escherichia coli; Funding; Generations; Genetic; Goals; Homology Modeling; Housing; Human; Image; Individual; Insecta; Laboratories; Light; Mammalian Cell; Membrane Proteins; Nature; New York; Numbers; Participant; Pathway interactions; Pharmacologic Substance; Photons; Production; Protein Structure Initiative; Protein phosphatase; Protozoan Proteins; Range; Rate; Reagent; Relative (related person); Research; Research Infrastructure; Research Personnel; Resolution; Resource Sharing; Resources; Robot; Roentgen Rays; Route; Sampling; Services; Solutions; Source; Spectrum Analysis; Structural Models; Structure; Synchrotrons; System; Technology; Tertiary Protein Structure; Therapeutic; Universities; X ray diffraction analysis; X-Ray Diffraction; base; beamline; college; day; design; expression cloning; globular protein; instrument; member; new technology; novel; programs; protein expression; protein structure; research study; solid state; structural biology; structural genomics

The Structural Biology Shared Resource provides the expertise needed to obtain high resolution structures by NMR and X-ray diffraction. These structural approaches are complemented by significant strengths in computational biology, cryo-electron microscopy, electron paramagnetic resonance, a wide range of vibrational spectroscopies and the development of novel macromolecular footprinting approaches at the College and AECC. The X-ray component has access to ~50 days/year on the X9A bending magnet beamline at the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory. An additional one day/month is currently available on the X29 insertion device at NSLS through the New York Structural Genomics Research Consortium (NYSGRC), along with access to the X4 bending magnet beamlines through Einstein's participation in the New York Structural Biology Center (NYSBC). The NYSGRC also provides access to the SGX-CAT beamline at the Advanced Photon Source, which offers FEDEX crystallographic service. These arrangements ensure that access to data collection resources is never rate limiting. There is also in-house Rigaku rotating anode/image plate data collection capabilities essential for characterization and pre-screening of samples prior to synchrotron analysis. The NMR component at AECC consists of 300 MHz and 600 MHz NMR spectrometers, one with a cryoprobe. In addition, AECC investigators have full access to the NYSBC which houses two new 900 MHz and three 800 MHz instruments both with cryoprobes, and one 750 MHz solid state NMR spectrometer. The Facility is now providing dedicated protein expression and purification expertise initially on E. coli expression systems due to the relative efficiency and throughput. It is anticipated that these efforts will be expanded to include higher order expression systems (i.e., insect and mammalian cell systems) and cell free expression systems. The enhancement of this service, as proposed in this application, is considered to be the single most direct route to increasing the utilization of the Structural Biology Shared Resource by AECC members. The capability of the Facility has been strengthened by the acquisition of a custom designed TECAN crystallization robot that can support the rapid (minutes) generation of a 96-well crystallization trial.

结构生物学共享资源提供了获得高分辨率结构所需的专业知识 由NMR和X射线衍射。这些结构方法得到了重大优势的补充 计算生物学，冷冻电子显微镜，电子顺磁共振，广泛的 振动光谱和新型大分子足迹的发展 大学和AECC。 X射线组件可以在X9A弯曲磁铁上访问〜50天/年 布鲁克黑文国家实验室的国家同步器光源（NSLS）的梁线。另一个 目前在NSLS的X29插入设备上通过纽约结构上可以找到一天/月 基因组学研究联盟（NYSGRC），以及访问X4弯曲磁铁梁 通过爱因斯坦参与纽约结构生物学中心（NYSBC）。 NYSGRC也是如此 提供对高级光子源的SGX-CAT光束线的访问，该光子源提供联邦快递 晶体学服务。这些安排确保访问数据收集资源永远不会率 限制。内部还有内部Rigaku旋转阳极/图像板数据收集功能对于 在进行同步加速器分析之前的样品表征和预筛查。 AECC的NMR组件 由300 MHz和600 MHz NMR光谱仪组成，一个带有冷冻探针。此外，AECC 调查人员可以完全访问NYSBC，该NYSBC包含两个新的900 MHz和三个800 MHz 具有冷冻探针的仪器和一个750 MHz固态NMR光谱仪。现在的设施 最初在大肠杆菌表达系统上提供专用的蛋白质表达和纯化专业知识 相对效率和吞吐量。预计这些努力将扩大到包括更高的 订单表达系统（即昆虫和哺乳动物细胞系统）和无细胞表达系统。这 在本应用程序中提出的那样，这项服务的增强被认为是最直接的路线 为了增加AECC成员对结构生物学共享资源的利用。功能 通过获取定制设计的Tecan结晶机器人，该设施得到了加强 可以支持96孔结晶试验的快速（分钟）生成。