Rapid Automated Fabrication of Tissues (RAFT): Corneal Stem Cells

组织快速自动化制造 (RAFT)：角膜干细胞

• 批准号: TS/G000611/1
• 负责人: Robert Brown
• 金额: $ 95.96万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=TS%2FG000611%2F1
• 关键词: Rapid; Automated; Fabrication; Tissues; RAFT

The goals are to develop an automated system for rapid, simple production of 3D tissue constructs and to demonstrate pre-clinical proof of concept using human corneal limbal stem cells to make corneal constructs. UCL's patented process is based on plastic compression of cast collagen gels. Tissue equivalents can be made in minutes with embossed 3D spatial niches suitable for controlling stem cell growth. The process addresses issues of high cost, low yield and long cycle times seen with standard culture approaches to tissue engineering. Test equipment will be built and used to investigate the impact of process parameters on structure and function. Methods for making multi-layer constructs with micro-nano surface embossed patterns will be developed. We aim to show that the cell-seeded construct is biomimetic (mimics real tissue), controls cell differentiation rates and supports corneal regeneration. The approach will be applicable to a range of therapeutic applications as well as drug and toxicity testing.

目标是开发一种自动化系统，用于快速、简单地生产 3D 组织结构，并展示使用人角膜缘干细胞制造角膜结构的临床前概念证明。伦敦大学学院的专利工艺基于铸造胶原蛋白凝胶的塑性压缩。通过适合控制干细胞生长的浮雕 3D 空间壁龛，可以在几分钟内制作出组织等效物。该工艺解决了组织工程标准培养方法中存在的高成本、低产量和长循环时间的问题。将建造并使用测试设备来研究工艺参数对结构和功能的影响。将开发制造具有微纳米表面压花图案的多层结构的方法。我们的目的是证明细胞接种结构是仿生的（模仿真实组织），控制细胞分化率并支持角膜再生。该方法将适用于一系列治疗应用以及药物和毒性测试。