Animal Care Core

动物护理核心

基本信息

批准号：
8204227
负责人：
JAMES D. MACY
金额：
$ 15.26万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-07-01 至
项目状态：
未结题

项目摘要

Precise metabolic phenotyping depends significantly on the health status of animal subjects. Stable housing and husbandry and microbiological testing minimize the influence of exogenous factors that could impact phenotyping results. The rigors inherent to quarantine and testing mice to ensure acceptable health status must be balanced with the need to expedite phenotyping of newly arrived mice. The Animal Core will provide state-of-the-art husbandry, health care, quarantine, testing, and procedure space for assessing mice submitted to the Mouse Metabolic Phenotyping Center (MMPC). The Animal Care Core has implemented an expedited quarantine program that has been effect for the past 9 years. This has successfully decreased the quarantine time and allowed phenotyping to begin while animals are within quarantine, allowing animals to be studied at younger ages and minimizing costs associated with the phenotyping process. The Core will: 1) import mice for metabolic phenotyping, 2) determine the microbiological profile of each imported cohort and provide the Center Director with interpretation and advice about the results, 3) provide stable, biocontainment housing, husbandry and health care for mice submitted to the Center, 4) provide a dedicated, biosecure suite of room for quarantine, housing and on-site phenotyping procedures, 5) perform morphological phenotyping as requested, 6) provide to Center clients results and advice on the health status of submitted mice. The facilities include 600 ft^ of dedicated biocontainment housing and procedure space equipped with 160 cage ventilated rack, an NMR analyzer, and 8 metabolic (calorimetry) cages. The core is expected to import 802 +/- 215 mice per year, based on core volume during the previous 5 years.

精确的代谢表型很大程度上取决于动物受试者的健康状况。稳定的饲养和饲养以及微生物检测可以最大限度地减少可能影响表型结果的外源因素的影响。为了确保可接受的健康状况而对小鼠进行检疫和测试所固有的严格性必须与加快新来小鼠表型分析的需要相平衡。动物核心将提供最先进的饲养、医疗保健、检疫、测试和程序空间，用于评估提交给小鼠代谢表型中心 (MMPC) 的小鼠。动物护理中心实施了一项快速检疫计划，该计划已生效 9 年。这成功地减少了检疫时间，并允许在动物检疫期间开始表型分析，从而使动物能够在更年轻的年龄进行研究，并最大限度地减少与表型分析过程相关的成本。核心将：1）进口小鼠进行代谢表型分析，2）确定每个进口群体的微生物特征，并向中心主任提供有关结果的解释和建议，3）为提交的小鼠提供稳定的生物防护住房、饲养和医疗保健向中心提供信息，4) 提供一套专用的生物安全房间用于检疫、居住和现场表型分析程序，5) 根据要求进行形态学表型分析，6) 向中心客户提供结果和对提交的小鼠的健康状况提出建议。这些设施包括 600 平方英尺的专用生物防护罩和操作空间，配备 160 个笼式通风架、一台 NMR 分析仪和 8 个代谢（量热）笼。根据过去 5 年的核心数量，该核心预计每年进口 802 +/- 215 只小鼠。