Equipping a newly renovated ABSL-3 suite with a "smart" biocontainment caging system

为新装修的 ABSL-3 套件配备“智能”生物防护笼系统

• 批准号: 10533643
• 负责人: JAMES D. MACY
• 金额: $ 40万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533643
• 关键词: 2019-nCoV; Accreditation; Animal Welfare; Animals; Censuses; Cessation of life; Clinical; Communicable Diseases; Consumption; Data; Decontamination; Disease; Emergency Situation; Epidemiology; Equipment and Supplies; Evaluation; Exposure to; Floods; Floor; Food; Frequencies; Funding; Future; Home; Housing; Human Resources; Individual; Laboratories; Locomotion; Medical Waste; Medical Waste Disposal; Monitor; Mus; Needles; Occupational Safety; Pathogenicity; Persons; Process; Progressive Disease; Public Health; Reducing Agents; Refuse Disposal; Research; Research Personnel; Risk; Rodent; Rotation; Safety; Soil; Source; Standardization; System; Time; Universities; Water; Work; animal data; animal facility; animal resource; biosafety level 3 facility; cost; density; flexibility; high efficiency particulate air filter; improved; pandemic disease; personal protective equipment; pressure; programs; seal; square foot; ventilation; wasting

ABSTRACT Animal Biosafety Level 3 (ABSL-3) studies have increase nearly 50% from pre-pandemic, in large part, by SARS- CoV-2 research and is now nearing ABSL-3 housing capacity limit in the current Yale Animal Resources Center (YARC) ABSL-3 facility. To remedy the ABSL-3 space shortfall, a $4.25M university-funded renovation of the Laboratory of Epidemiology and Public Health (LEPH) 8th floor animal facility includes restoring two previously decommissioned ABSL-3 animal suit to its original ABSL-3 use. Renovating LEPH 8 ABSL-3 suite to meet current ABSL-3 standards will provide basic criteria required for conducting ABSL-3 work but the renovation does not “move the needle” towards more efficient and safer rodent ABSL-3 research. YARC proposes to equip two LEPH 8 ABSL-3 animal rooms with a total of five Tecniplast DVC® IsoCage N biocontainment cage system (and one rotator) with a newly available home-cage monitoring system to fully leverage use of the new ABSL-3 suite. These “smart” biocontainment cages will transform use of the new ABSL-3 suite by 1) adding an additional level of biocontainment for maximum personnel protection through sealed, negative pressure caging system with cage level HEPA filtration. This additional cage level safeguard will lessen concerns over the use of multiple BSL-3 agents within one room, thereby maximizing the flexibility of the two animal rooms; 2) automated evaluation of cage bedding conditions (wetness) will result in objective data that quantifies when cage changing is required. This system standardizes cage conditions and avoids unnecessary cage changes, given static cage are typically changed on a 7-day cycle, regardless of need. The ventilation combined with automated cage wetness indicator will extend the cage change cycle to 14 days and beyond, especially for cages with lower numbers and/or smaller mice. The “change on demand” approach reduces labor by providing objective bedding condition data, potential exposure to BSL-3 agents during cage changes from reduced cage changes and decreases the amount of waste generated (up to 50% less). The wetness indictor system will also send emergency alerts if cage flooding is detected from the water source (or otherwise), guarding against catastrophic animal loss; and 3) automatic and continuous monitoring and analysis of spontaneous animal locomotion resulting in enhanced animal welfare monitoring. Since most ABSL-3 studies result in clinical disease that correlates with activity levels, the ability to continuously monitor activity remotely will revolutionize animal monitoring. Investigators will be able to target when in-person monitoring is needed, better pinpoint endpoints to subvert unnecessary animal (and data) loss and decrease the amount of unproductive time and personal risk associated with unnecessary/excessive entry into ABSL-3 space. Taken together, occupational safety, housing efficiency and animal monitoring stringency will be increased while labor, PPE and medical waste disposal costs will be decreased.

抽象的 动物生物安全 3 级 (ABSL-3) 研究较大流行前增加了近 50%，这在很大程度上是由于 SARS- 耶鲁大学动物资源中心正在进行 CoV-2 研究，目前已接近 ABSL-3 住房容量限制 (YARC) ABSL-3 设施 为​​了弥补 ABSL-3 空间短缺，大学资助了 425 万美元进行改造。 流行病学和公共卫生实验室 (LEPH) 8 楼动物设施包括修复两个以前的动物设施 退役的 ABSL-3 动物套装恢复原来的 ABSL-3 用途 翻新 LEPH 8 ABSL-3 套件以满足当前需求。 ABSL-3 标准将提供进行 ABSL-3 工作所需的基本标准，但翻修不会 “推动”更多、更安全的啮齿动物 ABSL-3 研究，YARC 提议装备两台 LEPH。 8 个 ABSL-3 动物房，共有 5 个 Tecniplast DVC® IsoCage N 生物防护笼系统（以及 1 个 旋转器）与新推出的家用笼式监控系统，以充分利用新的 ABSL-3 套件。 “智能”生物防护笼将通过以下方式改变新的 ABSL-3 套件的使用： 1) 添加额外的级别 生物防护通过带笼子的密封负压笼系统提供最大程度的人员保护 这种额外的笼级防护措施将减少对使用多个 BSL-3 的担忧。 2）自动化评估； 笼子垫料条件（湿度）将产生客观数据，可量化何时需要更换笼子。 该系统标准化了笼子条件并避免了不必要的笼子更换，因为静态笼子通常是 无论是否需要，通风与自动笼湿度指示器相结合，以 7 天为周期进行更改。 将延长笼子更换周期至 14 天及更长，特别是对于数量较少和/或更小的笼子 “按需改变”方法通过提供客观的垫料条件数据、潜力来减少劳动力。 减少笼子更换期间接触 BSL-3 制剂并减少废物量 如果笼子进水，湿度指示器系统也会发送紧急警报。 从水源（或其他）检测，防止灾难性的动物损失；以及 3) 自动和 持续监测和分析动物自发运动，从而提高动物福利 由于大多数 ABSL-3 研究导致与活动水平相关的临床疾病，因此无法进行监测。 持续远程监控活动将彻底改变动物监控，研究人员将能够确定目标。 需要亲自监测，更好地查明终点，以消除不必要的动物（和数据）损失和 减少与不必要/过度进入相关的非生产时间和个人风险 ABSL-3 空间综合考虑，职业安全、住房效率和动物监测的严格性。 增加，而劳动力、个人防护装备和医疗废物处理成本将下降。