PRIMATE GENETIC ANALYSIS AND PEDIGREE MANAGEMENT

灵长类动物遗传分析和谱系管理

• 批准号: 8172351
• 负责人: JAMES GIBSON ELSE
• 金额: $ 10.97万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172351
• 关键词: Acquired Immunodeficiency Syndrome; Animals; Birth; Breeding; Cercocebus; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Data Set; Databases; Funding; Genetic; Genetic Markers; Genotype; Grant; Institution; Macaca; Macaca mulatta; Microsatellite Repeats; Primates; Records; Research; Research Personnel; Resources; Sampling; Source; System; United States National Institutes of Health; Veterinarians; cohort; genetic analysis; genetic pedigree; meetings; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Samples for DNA extraction to determine parentage have been collected from 1,409 rhesus macaque comprising the Yerkes' breeding colony of 1,691. This includes 614 animals from the SPF colonies dedicated to AIDS research, and 795 animals from NSPF animals. Each of these samples has been genotyped for 19 highly polymorphic microsatellites spread throughout the autosomal chromosomes. In addition 282 samples from the 2008 birth cohort and 462 samples from the 2009 birth cohort have been collected, and genotyping efforts are currently underway for these samples. These data are used to determine parentage, pedigree, and selected genetic markers for all of our macaques and mangabeys maintained at the Field Station. The ability to characterize specific genetic components has enabled us to better meet specific investigator needs, develop more diverse research endeavors, to selectively breed for specific genetic traits, and to undertake specific phenotypic comparisons. To manage this data set, we are establishing a full-scale database system that will be able to assimilate genetic, parentage, pedigree, and demographic variables on all the animals. This database system will be incorporated into our current animal records system, which will enable Yerkes' investigators and veterinarians to track, manage and view an animal's record with a single query.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 从1,409个恒河猕猴中收集了DNA提取的样品，以确定父母的育儿，其中包括Yerkes的繁殖菌落为1,691。 其中包括来自专门用于艾滋病研究的SPF菌落的614只动物，以及来自NSPF动物的795只动物。这些样品中的每一个都针对19种高度多态微卫星的基因分型，分布在整个常染色体染色体中。此外，还收集了来自2008年出生队列的282个样本和2009年出生队列的462个样本，目前正在为这些样品进行基因分型研究。 这些数据用于确定我们在现场站维护的所有猕猴和芒果的亲属，谱系和选定的遗传标记。 表征特定遗传成分的能力使我们能够更好地满足特定的研究者需求，发展更多样化的研究努力，有选择地培养特定的遗传特征，并进行特定的表型比较。 为了管理此数据集，我们正在建立一个全尺度数据库系统，该系统将能够在所有动物上吸收遗传，亲子，血统和人口统计学变量。 该数据库系统将被整合到我们当前的动物记录系统中，这将使Yerkes的调查人员和兽医能够通过单个查询跟踪，管理和查看动物的记录。