Super-Resolution Structured Illumination Microscope (SIM)

超分辨率结构照明显微镜 (SIM)

• 批准号: 7793345
• 负责人: Derek K. Toomre
• 金额: $ 49.68万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-10 至 2011-06-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793345
• 关键词: Area; Arts; Biological; Biomedical Research; Cell physiology; Cells; Cellular biology; Communities; Custom; Cytoskeleton; Data; Development; Diabetes Mellitus; Electron Microscope; Endocrinology; Funding; Genetic; Growth Cones; Image; Lead; Life; Light; Lighting; Link; Malignant Neoplasms; Membrane; Membrane Protein Traffic; Microscope; Microscopy; Neurobiology; Neurons; Organelles; Postdoctoral Fellow; Research; Resolution; Structure; Synapses; System; Training; Vesicle; cellular imaging; insight; instrument; light microscopy; medical schools; neuropathology; novel; single molecule

DESCRIPTION (provided by applicant): Cell Biology is intimately linked with the ability to see organelles, vesicles, cytoskeleton and even single molecules inside cells, both in normal and diseased states. Instruments that allow biologists to see better, such as the confocal and electron microscope have lead to major paradigm shifts of how cells function. Recently a new revolution in imaging has emerged, called 'super- resolution microscopy' which has the power to break the Abbe's 'light barrier' of what can be resolved by far field light microscopy. The potential here is enormous (akin to confocal microscopes two decades ago) and has the power to resolve objects in living cells (or fixed) with all the power of conventional fluorescent tags and the rainbow of GFP probes. Our consortium of users from cell biology, endocrinology, neurobiology and genetics, whom are experts in their respective fields, request funds to purchase a state-of-the-art super-resolution structured illumination microscope (SIM) that was very recently commercialized by Zeiss. Preliminary data indicate that the SIM system will double the resolution in X, Y, and Z axis and give almost 8-fold better volumetric resolution than conventional confocal microscopes. Our applications are well-matched to capitalize on this enhanced resolution so as to gain new insight into how cells function in both their native state and in diabetes, cancer and neuropathologies. Our consortium's projects focus on three research areas: 1) imaging of neurons, synapses and growth cones; 2) imaging of membranes and membrane traffic; 3) imaging of the cell cytoskeleton. Our aim is to make SIM available to a large group of users at Yale School of Medicine and the larger scientific community. The system will be set up in high-end custom space the 'CINEMA' center (Cellular Imaging using New Microscopy Approaches) under the guidance of Dr. Toomre, an expert in the cell- biological application of novel microscopes. It will be properly maintained by a well-trained Research Associate in the lab (50% effort) with additional backup provided by Dr. Bewersdorf's lab which specializes on super-resolution microscopy development and application. The requested instrument is expected to be the first of its kind in the USA. We believe this instrument will open up new avenues in cell biology and stimulate the establishment of super- resolution microscopy as a new standard in biomedical research.

描述（由申请人提供）：细胞生物学与在正常和患病状态下看到细胞器，囊泡，细胞骨架，甚至单分子的能力密切相关。允许生物学家看到更好的仪器，例如共聚焦和电子显微镜，导致细胞功能的主要范式转移。最近，出现了一场新的成像革命，称为“超级分辨率显微镜”，它具有打破Abbe的“轻屏障”的力量，可以通过远处的田间光学显微镜解决。这里的潜力是巨大的（类似于二十年前的共聚焦显微镜），并且具有用常规荧光标签的所有功能和GFP探针的彩虹解决活细胞（或固定）中的物体的能力。 我们来自细胞生物学，内分泌学，神经生物学和遗传学的用户联盟，他们是各自领域的专家，要求资金购买最先进的超分辨率的结构化照明显微镜（SIM），该显微镜（SIM）最近由Zeiss商业化。 。初步数据表明，SIM系统将在X，Y和Z轴中的分辨率增加一倍，并且比常规共聚焦显微镜获得近8倍更好的容量分辨率。我们的应用非常匹配，可以利用这种增强的分辨率，以便对细胞在其天然状态和糖尿病，癌症和神经病理学的作用方面进行新的见解。我们的财团项目集中在三个研究领域：1）神经元，突触和生长锥的成像； 2）膜和膜交通的成像； 3）细胞细胞骨架的成像。我们的目的是使耶鲁大学医学院和更大科学界的大批用户可以使用SIM卡。 该系统将在高端自定义空间中建立在“电影”中心（使用新显微镜方法的细胞成像）的指导下，Toomre博士是新型显微镜的细胞生物应用专家。 Bewersdorf博士实验室提供的其他备份将由实验室中训练有素的研究助理（50％的努力）妥善维护，该实验室专门从事超分辨率显微镜开发和应用。 所请求的仪器预计将是美国第一个同类仪器。我们认为，该仪器将开辟细胞生物学的新途径，并刺激超级分辨率显微镜作为生物医学研究的新标准。