DEVELOPMENT OF MONKEYS PRODUCED BY EMBRYO SPLITTING & SPERM INJECTION

胚胎分裂产生的猴子的发育

• 批准号: 7349356
• 负责人: GENE P. SACKETT
• 金额: $ 13.2万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349356
• 关键词: DEVELOPMENT; MONKEYS; PRODUCED; EMBRYO; SPLITTING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Progress in experimentally studying the functions of genes discovered now or in the future depends primarily on having appropriate animal models. Similarly, learning how to manipulate genes to alter anatomy, physiology, or behavior, and determining the safety of these manipulations in primates, including humans, depends on appropriate animal models. An important approach to these issues concerns controlling and manipulating the genes of identical twins, who at conception have an identical genome. Recently, a procedure has been developed in which the 4-8 cell embryo of primates (morula stage) is split into individual cells. These cells are implanted into surrogate mothers, who carry a single fetus to term. The surrogate mother may or may not be the actual biological mother. Given appropriate experimental techniques, these identical twin fetuses can serve as control and experimental subjects in studies manipulating almost any aspect of the genome, the mother, the fetal environment, the subject's anatomy and physiology, and/or the postnatal environment. The utility of these potential models using embryo split twins depends largely on exactly how similar the twins are in their anatomy, growth, physiology, and behavior. The two major purposes of this research are (1) to determine the degree of twin similarity in macaque monkey subjects across a wide range of growth, physiological, and behavioral characteristics, and (2) determine whether the embryo splitting and surrogate mothering procedure produces any abnormalities in these characteristics.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。现在或将来发现的基因功能的实验研究进展主要取决于合适的动物模型。同样，学习如何操纵基因来改变解剖学、生理学或行为，并确定这些操纵在灵长类动物（包括人类）中的安全性，取决于适当的动物模型。解决这些问题的一个重要方法涉及控制和操纵同卵双胞胎的基因，这些双胞胎在受孕时具有相同的基因组。最近，开发了一种程序，将灵长类动物的 4-8 细胞胚胎（桑葚胚阶段）分裂成单个细胞。这些细胞被植入代孕母亲体内，代孕母亲怀上一个胎儿直至足月。代孕母亲可能是也可能不是实际的亲生母亲。如果采用适当的实验技术，这些同卵双胞胎胎儿可以作为研究中的对照和实验对象，操纵基因组、母亲、胎儿环境、受试者的解剖学和生理学和/或产后环境的几乎任何方面。这些使用胚胎分裂双胞胎的潜在模型的实用性很大程度上取决于双胞胎在解剖学、生长、生理学和行为方面的相似程度。这项研究的两个主要目的是（1）确定猕猴受试者在广泛的生长、生理和行为特征方面的双胞胎相似程度，以及（2）确定胚胎分裂和代孕程序是否会产生任何影响。这些特征的异常。